Visualization by atomic force microscopy and FISH of the 45S rDNA gaps in mitotic chromosomes of Lolium perenne

Protoplasma

Volumn 236, Issue 1-4, 2009, Pages 59-65

  Huang, Jing ^a   Ma, Lu ^a   Sundararajan, Sriram ^b   Fei, Shui zhang ^c   Li, Lijia ^a  

^a WUHAN UNIVERSITY   (China)

^b Iowa State University of Science and Technology   (United States)

^c IOWA STATE UNIVERSITY   (United States)

Author keywords

45S rDNA; AFM; Chromatin decondensation; Chromosome gap and break; Fluorescence in situ hybridization; Lolium

Indexed keywords

LOLIUM; LOLIUM PERENNE;

RIBOSOME DNA;

ARTICLE; ATOMIC FORCE MICROSCOPY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; LOLIUM; METHODOLOGY; MITOSIS; PLANT CHROMOSOME;

CHROMOSOMES, PLANT; DNA, RIBOSOMAL; IN SITU HYBRIDIZATION, FLUORESCENCE; LOLIUM; MICROSCOPY, ATOMIC FORCE; MITOSIS;

EID: 70349336782     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-009-0051-x     Document Type: Article

References (30)

1. Argueso JL, Westmoreland J, Mieczkowski PA, Gawel M, Petes TD, Resnick MA (2008) Double-strand breaks associated with repetitive DNA can reshape the genome. Proc Natl Acad Sci U S A 105:11845-11850. doi:10.1073/pnas.0804529105.
2. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208-218. doi:10.1007/BF02672069.
3. Caperta AD, Neves N, Viegas W, Pikaard CS, Preuss S (2007) Relationships between transcription, silver staining, and chromatin organization of nucleolar organizers in Secale cereale. Protoplasma 232:55-59. doi:10.1007/s00709-007-0277-4.
4. Casper AM, Nghiem P, Arlt MF, Glover TW (2002) ATR regulates fragile site stability. Cell 111:779-789. doi:10.1016/S0092-8674(02)01113-3.
5. Casper AM, Mieczkowski PA, Gawel M, Petes TD (2008) Low Levels of DNA Polymerase Alpha Induce Mitotic andMeiotic Instability in the Ribosomal DNA Gene Cluster of Saccharomyces cerevisiae. PLoS Genet 4:e1000105. doi:10.1371/journal.pgen.1000105.
6. Gericke GS (1999) Chromosomal fragility may be indicative of altered higher-order DNA organization as the underlying genetic diathesis in complex neurobehavioural disorders. Med Hypotheses 52:201-208. doi:10.1054/mehy.1997.0643.
7. Glover TW (2006) Common fragile sites. Cancer Lett 232:4-12. doi:10.1016/j.canlet.2005.08.032.
8. Harrison CJ, Jack EM, Allen TD, Harris R (1983) The fragile X: A scanning electron microscope study. J Med Genet 20:280-285. doi:10.1136/jmg.20.4.280.
9. Heslop-Harrison JS (2000) Comparative genome organization in plants: From sequence and markers to chromatin and chromosomes. Plant Cell 12:617-635.
10. Huang J, Ma L, Yang F, Fei SZ, Li L (2008) 45S rDNA regions are chromosome fragile sites expressed as gaps in vitro on metaphase chromosomes of root-tip meristematic cells in Lolium spp. PLoS One 3:e2167. doi:10.1371/journal.pone.0002167.
11. Iwabuchi S, Muramatsu H, Chiba N, Kinjo Y, Murakami Y, Sakaguchi T, Yokoyama K, Tamiya E (1997) Simultaneous detection of near-field topographic and fluorescence images of human chromosomes via scanning near-field optical/atomic-force microscopy (SNOAM). Nucleic Acids Res 25:1662-1663. doi:10.1093/nar/25.8.1662.
12. Karuppiah KSK, Sundararajan S, Xu ZH, Li X (2006) The effect of protein adsorption on the friction behavior of ultra-high molecular weight polyethylene. Tribol Lett 22:181-188. doi:10.1007/s11249-006-9078-8.
13. Kim HM, Narayanan V, Mieczkowski PA, Petes TD, Krasilnikova MM, Mirkin SM, Lobachev KS (2008) Chromosome fragility at GAA tracts in yeast depends on repeat orientation and requires mismatch repair. EMBO J 27:2896-2906. doi:10.1038/emboj.2008.205.
14. Li LJ, Arumuganathan K, Rines HW, Phillips RL, Riera-Lizarazu O, Sandhu D, Zhou Y, Gill KS (2001) Flow cytometric sorting of maize chromosome 9 from an oat-maize chromosome addition line. Theor Appl Genet 102:658-663. doi:10.1007/s001220051694.
15. Lodish H, Berk A, Zipursky SL, Matsudaita P, Baltimore D, Darnell J (2000) Molecular cell biology, 4th edn. Freeman, New York, p 326.
16. Moers MH, Kalle WH, Ruiter AG, Wiegant JC, Raap AK, Greve J, de Grooth BG, van Hulst NF (1996) Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy. J Microsc 182:40-45.
17. Naganowska B, Zielinska A (2004) Localisation of rDNA in the Lupinus genome during the cell cycle. J Appl Genet 45:189-193.
18. Nelson DL (1995) The fragile X syndromes. Semin Cell Biol 6:5-11. doi:10.1016/1043-4682(95)90009-8.
19. Popescu NC (2003) Genetic alterations in cancer as a result of breakage at fragile sites. Cancer Lett 192:1-17. doi:10.1016/S0304-3835(02)00596-7.
20. Richards RI (2001) Fragile and unstable chromosomes in cancer: Causes and consequences. Trends Genet 17:339-345. doi:10.1016/S0168-9525(01)02303-4.
21. Schaper A, Rössle M, Formanek H, Jovin TM, Wanner G (2000) Complementary visualization of mitotic barley chromatin by field-emission scanning electron microscopy and scanning force microscopy. J Struct Biol 129:17-29. doi:10.1006/jsbi.1999.4203.
22. Scheid W, Traut H (1971) Visualization by scanning electron microscopy of achromatic lesions (gaps) induced by x-rays in chromosomes of Vicia faba. Mutat Res 11:253-255.
23. Shichiri M, Fukushi D, Sugiyama S, Yoshino T, Ohtani T (2003) Analysis by atomic force microscopy of morphological changes in barley chromosomes during FISH treatment. Chromosome Res 11:65-71. doi:10.1023/A:1022062100358.
24. Song YC, Gustafson JP (1995) The physical location of 14 RFLP markers in rice (Oryza ativa L.). Theor Appl Genet 90:113-119.
25. Tamayo J, Miles M (2000) Human chromosome structure studied by scanning force microscopy after an enzymatic digestion of the covering cell material. Ultramicroscopy 82:245-251. doi:10.1016/S0304-3991(99)00125-4.
26. Thomas HM, Harper JA, Meredith MR, Morgan WG, Thomas ID, Timms E, King IP (1996) Comparison of ribosomal DNA sites in Lolium species by fluorescencein situ hybridization. Chromosome Res 4:486-490. doi:10.1007/BF02261775.
27. Wang YH (2006) Chromatin structure of human chromosomal fragile sites. Cancer Lett 232:70-78. doi:10.1016/j.canlet.2005.07.040.
28. Wen GY, Jenkins EC, Goldberg EM, Genovese M, Brown WT, Wisniewski HM (1999) Ultrastructure of the fragile X chromosome: New observations on the fragile site. Am J Med Genet 83:331-333. doi:10.1002/(SICI)1096-8628(19990402)83:<331::AID-AJMG21>3.0.CO;2-U.
29. Woodcock CL, Horowitz RA (1995) Chromatin organization reviewed. Trends Cell Biol 5:272-277. doi:10.1016/S0962-8924(00)89038-8.
30. Yoshino T, Sugiyama S, Hagiwara S, Fukushi D, Shichiri M, Nakao H, Kim JM, Hirose T, Muramatsu H, Ohtani T (2003) Nano-scale imaging of chromosomes and DNA by scanning near-field optical/atomic force microscopy. Ultramicroscopy 97:81-87. doi:10.1016/S0304-3991(03)00032-9.