Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice

Toxicon

Volumn 54, Issue 6, 2009, Pages 793-801

  Villanova, Fabiola E ^a   Andrade, Enrico ^a   Leal, Élcio ^b   Andrade, Priscila M ^a   Borra, Ricardo C ^c   Troncone, Lanfranco R P ^d   Magalhães, Leandro ^d   Leite, Katia R M ^a   Paranhos, Mario ^a   Claro, Joaquim ^a   Srougi, Miguel ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^c IBIRAPUERA UNIVERSITY   (Brazil)

^d INSTITUTO BUTANTAN   (Brazil)

Author keywords

Gene expression; Nitric oxide; Penile erection; Phoneutria nigriventer; Spider toxin; Tx2 6

Indexed keywords

ABC TRANSPORTER; ABC TRANSPORTER C1; ARGININE; CYCLIC GMP; CYCLIN DEPENDENT KINASE INHIBITOR 1A; ENDOTHELIN B RECEPTOR; FERRITIN; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; OSTEONECTIN; PROTEIN BCL 2; PROTEIN S 100; PROTEIN S 100A10; RANTES; SPIDER VENOM; SUPEROXIDE DISMUTASE; TRANSCRIPTION FACTOR JUNB; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; CORPUS CAVERNOSUM; ENZYME ACTIVATION; GENE EXPRESSION PROFILING; GENE OVEREXPRESSION; INOCULATION; MALE; MICROARRAY ANALYSIS; MOLECULAR MECHANICS; MOUSE; MUSCLE RELAXATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PENIS ERECTION; PRIAPISM; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SPIDER;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; GENE EXPRESSION PROFILING; MALE; MICE; NITRIC OXIDE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PENILE ERECTION; PEPTIDES; POLYMERASE CHAIN REACTION; SPIDER VENOMS;

ARANEAE; MAMMALIA; MUS; PHONEUTRIA NIGRIVENTER;

EID: 68949162054     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2009.06.006     Document Type: Article

References (44)

1. Agarwal A., Nandipati K.C., Sharma R.K., Zippe C.D., and Raina R. Role of oxidative stress in the pathophysiological mechanism of erectile dysfunction. J. Androl. 27 3 (2006) 335-347
2. Andrade E., Villanova F., Borra P., Leite K., Troncone L., Cortez I., Messina L., Paranhos M., Claro J., and Srougi M. Penile erection induced in vivo by a purified toxin from the Brazilian spider Phoneutria nigriventer. BJU Int. 102 7 (2008) 835-837
3. Araujo D.A., Cordeiro M.N., Diniz C.R., and Beirao P.S. Effects of a toxic fraction, PhTx2, from the spider Phoneutria nigriventer on the sodium current. Naunyn Schmiedebergs Arch. Pharmacol. 347 2 (1993) 205-208
4. Barker T.H., Baneyx G., Cardo-Vila M., Workman G.A., Weaver M., Menon P.M., Dedhar S., Rempel S.A., Arap W., Pasqualini R., Vogel V., and Sage E.H. SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity. J. Biol. Chem. 280 43 (2005) 36483-36493
5. Bradshaw A.D., Francki A., Motamed K., Howe C., and Sage E.H. Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation. Mol. Biol. Cell 10 5 (1999) 1569-1579
6. Brazil V.V.J. Imunização por via intradérmica e por via subcutânea pelo sôro antictenus. Método de dosagem e primeira aplicação no homem. Bras. Med. 60 5 (1925) 555-563
7. Bucaretchi F., Deus Reinaldo C.R., Hyslop S., Madureira P.R., De Capitani E.M., and Vieira R.J. A clinico-epidemiological study of bites by spiders of the genus Phoneutria. Rev. Inst. Med. Trop. Sao Paulo 42 1 (2000) 17-21
8. Bucaretchi F., Mello S.M., Vieira R.J., Mamoni R.L., Blotta M.H., Antunes E., and Hyslop S. Systemic envenomation caused by the wandering spider Phoneutria nigriventer, with quantification of circulating venom. Clin. Toxicol. (Phila.) 46 9 (2008) 885-889
9. Burnett A.L. Nitric oxide in the penis: physiology and pathology. J. Urol. 157 1 (1997) 320-324
10. Cao Z., Wu H.K., Bruce A., Wollenberg K., and Panjwani N. Detection of differentially expressed genes in healing mouse corneas, using cDNA microarrays. Invest. Ophthalmol. Vis. Sci. 43 9 (2002) 2897-2904
11. Christ G.J., Lerner S.E., Kim D.C., and Melman A. Endothelin-1 as a putative modulator of erectile dysfunction: I. Characteristics of contraction of isolated corporal tissue strips. J. Urol. 153 6 (1995) 1998-2003
12. Cordeiro Mdo N., de Figueiredo S.G., Valentim Ado C., Diniz C.R., von Eickstedt V.R., Gilroy J., and Richardson M. Purification and amino acid sequences of six Tx3 type neurotoxins from the venom of the Brazilian armed' spider Phoneutria nigriventer. Toxicon 31 1 (1993) 35-42
13. Cordeiro Mdo N., Diniz C.R., Valentim Ado C., von Eickstedt V.R., Gilroy J., and Richardson M. The purification and amino acid sequences of four Tx2 neurotoxins from the venom of the Brazilian 'armed' spider Phoneutria nigriventer (Keys). FEBS Lett. 310 2 (1992) 153-156
14. Corzo G., and Escoubas P. Pharmacologically active spider peptide toxins. Cell Mol. Life Sci. 60 11 (2003) 2409-2426
15. Corzo G., Escoubas P., Villegas E., Karbat I., Gordon D., Gurevitz M., Nakajima T., and Gilles N. A spider toxin that induces a typical effect of scorpion alpha-toxins but competes with beta-toxins on binding to insect sodium channels. Biochemistry 44 5 (2005) 1542-1549
16. de Paula Le Sueur L., Kalapothakis E., and da Cruz-Hofling M.A. Breakdown of the blood-brain barrier and neuropathological changes induced by Phoneutria nigriventer spider venom. Acta Neuropathol. 105 2 (2003) 125-134
17. Dean R.C., and Lue T.F. Physiology of penile erection and pathophysiology of erectile dysfunction. Urol. Clin. North Am. 32 4 (2005) 379-395
18. Diaz J.H. The global epidemiology, syndromic classification, management, and prevention of spider bites. Am. J. Trop. Med. Hyg. 71 2 (2004) 239-250
19. Dos Santos R.G., Van Renterghem C., Martin-Moutot N., Mansuelle P., Cordeiro M.N., Diniz C.R., Mori Y., De Lima M.E., and Seagar M. Phoneutria nigriventer omega-phonetoxin IIA blocks the Cav2 family of calcium channels and interacts with omega-conotoxin-binding sites. J. Biol. Chem. 277 16 (2002) 13856-13862
20. Edman P. A method for the determination of amino acid sequence in peptides. Arch. Biochem. 22 3 (1949) 475
21. Filippi S., Marini M., Vannelli G.B., Crescioli C., Granchi S., Vignozzi L., Luconi M., Ferruzzi P., Morelli A., Forti G., Ledda F., and Maggi M. Effects of hypoxia on endothelin-1 sensitivity in the corpus cavernosum. Mol. Hum. Reprod. 9 12 (2003) 765-774
22. Granchi S., Vannelli G.B., Vignozzi L., Crescioli C., Ferruzzi P., Mancina R., Vinci M.C., Forti G., Filippi S., Luconi M., Ledda F., and Maggi M. Expression and regulation of endothelin-1 and its receptors in human penile smooth muscle cells. Mol. Hum. Reprod. 8 12 (2002) 1053-1064
23. Jin L., and Burnett A.L. RhoA/Rho-kinase in erectile tissue: mechanisms of disease and therapeutic insights. Clin. Sci. (Lond.) 110 2 (2006) 153-165
24. Kalapothakis E., Penaforte C.L., Leao R.M., Cruz J.S., Prado V.F., Cordeiro M.N., Diniz C.R., Romano-Silva M.A., Prado M.A., Gomez M.V., and Beirao P.S. Cloning, cDNA sequence analysis and patch clamp studies of a toxin from the venom of the armed spider (Phoneutria nigriventer). Toxicon 36 12 (1998) 1971-1980
25. Kessler C.B., and Delany A.M. Increased Notch 1 expression and attenuated stimulatory G protein coupling to adenylyl cyclase in osteonectin-null osteoblasts. Endocrinology 148 4 (2007) 1666-1674
26. Le Sueur L.P., Collares-Buzato C.B., and da Cruz-Hofling M.A. Mechanisms involved in the blood-brain barrier increased permeability induced by Phoneutria nigriventer spider venom in rats. Brain Res. 1027 1-2 (2004) 38-47
27. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25 4 (2001) 402-408
28. Maas R., Schwedhelm E., Albsmeier J., and Boger R.H. The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function. Vasc. Med. 7 3 (2002) 213-225
29. Martin-Moutot N., Mansuelle P., Alcaraz G., Dos Santos R.G., Cordeiro M.N., De Lima M.E., Seagar M., and Van Renterghem C. Phoneutria nigriventer toxin 1: a novel, state-dependent inhibitor of neuronal sodium channels that interacts with {micro} conotoxin binding sites. Mol. Pharmacol. 69 6 (2006) 1931-1937
30. Matavel A., Cruz J.S., Penaforte C.L., Araujo D.A., Kalapothakis E., Prado V.F., Diniz C.R., Cordeiro M.N., and Beirao P.S. Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2-6. FEBS Lett. 523 1-3 (2002) 219-223
31. Matavel A., Fleury C., Oliveira L.C., Molina F., de Lima M.E., Cruz J.S., Cordeiro M.N., Richardson M., Ramos C.H., and Beirao P.S. Structure and activity analysis of two spider toxins that alter sodium channel inactivation kinetics. Biochemistry 48 14 (2009) 3078-3088
32. Musicki B., and Burnett A.L. eNOS function and dysfunction in the penis. Exp. Biol. Med. (Maywood) 231 2 (2006) 154-165
33. Napoli C., and Ignarro L.J. Nitric oxide-releasing drugs. Annu. Rev. Pharmacol. Toxicol. 43 (2003) 97-123
34. Nunes K.P., Costa-Goncalves A., Lanza L.F., Cortes S.F., Cordeiro M.N., Richardson M., Pimenta A.M., Webb R.C., Leite R., and De Lima M.E. Tx2-6 toxin of the Phoneutria nigriventer spider potentiates rat erectile function. Toxicon 51 7 (2008) 1197-1206
35. Parkkisenniemi U.M., and Klinge E. Functional characterization of endothelin receptors in the bovine retractor penis muscle and penile artery. Pharmacol. Toxicol. 79 2 (1996) 73-79
36. Pinheiro A.C., Gomez R.S., Massensini A.R., Cordeiro M.N., Richardson M., Romano-Silva M.A., Prado M.A., De Marco L., and Gomez M.V. Neuroprotective effect on brain injury by neurotoxins from the spider Phoneutria nigriventer. Neurochem. Int. 49 5 (2006) 543-547
37. Rego E., Bento A.C., Lopes-Martins R.A., Antunes E., Novello J.C., Marangoni S., Giglio J.R., Oliveira B., and De Nucci G. Isolation and partial characterization of a polypeptide from Phoneutria nigriventer spider venom that relaxes rabbit corpus cavernosum in vitro. Toxicon 34 10 (1996) 1141-1147
38. Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S., Lima M.E., Figueiredo S.G., Bloch Jr. C., Vasconcelos E.A., Campos F.A., Gomes P.C., and Cordeiro M.N. Comparison of the partial proteomes of the venoms of Brazilian spiders of the genus Phoneutria. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 142 3-4 (2006) 173-187
39. Sasaki Y., Asamizu E., Shibata D., Nakamura Y., Kaneko T., Awai K., Amagai M., Kuwata C., Tsugane T., Masuda T., Shimada H., Takamiya K., Ohta H., and Tabata S. Monitoring of methyl jasmonate-responsive genes in Arabidopsis by cDNA macroarray: self-activation of jasmonic acid biosynthesis and crosstalk with other phytohormone signaling pathways. DNA Res. 8 4 (2001) 153-161
40. Schiemann B.J., Neil J.R., and Schiemann W.P. SPARC inhibits epithelial cell proliferation in part through stimulation of the transforming growth factor-beta-signaling system. Mol. Biol. Cell 14 10 (2003) 3977-3978
41. Shi Q., Bao S., Song L., Wu Q., Bigner D.D., Hjelmeland A.B., and Rich J.N. Targeting SPARC expression decreases glioma cellular survival and invasion associated with reduced activities of FAK and ILK kinases. Oncogene 26 28 (2007) 4084-4094
42. Sullivan C.J., Teal T.H., Luttrell I.P., Tran K.B., Peters M.A., and Wessells H. Microarray analysis reveals novel gene expression changes associated with erectile dysfunction in diabetic rats. Physiol. Genomics 23 2 (2005) 192-205
43. Troncone L.R.P., Georgiou J., Hua S.Y., Elrick D., Lebrun I., Magnoli F., and Charlton M.P. Promiscuous and reversible blocker of presynaptic calcium channels in frog and crayfish neuromuscular junctions from Phoneutria nigriventer spider venom. J. Neurophysiol. 90 5 (2003) 3529-3537
44. Yonamine C.M., Troncone L.R., and Camillo M.A. Blockade of neuronal nitric oxide synthase abolishes the toxic effects of Tx2-5, a lethal Phoneutria nigriventer spider toxin. Toxicon 44 2 (2004) 169-172