Phoneutria nigriventer toxin 1: A novel, state-dependent inhibitor of neuronal sodium channels that interacts with μ conotoxin binding sites

Molecular Pharmacology

Volumn 69, Issue 6, 2006, Pages 1931-1937

  Martin Moutot, Nicole ^a,c   Mansuelle, Pascal ^b,c   Alcaraz, Gisèle ^a,c   Dos Santos, Raquel Gouvêa ^d   Cordeiro, Marta N ^d   De Lima, Maria Elena ^d   Seagar, Michael ^a,c   Van Renterghem, Catherine ^a,c  

^a INSERM   (France)

^b CNRS   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

^d FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CONOTOXIN; GLYCINE; SODIUM CHANNEL; SODIUM CHANNEL BLOCKING AGENT; TETRODOTOXIN; TX 1; UNCLASSIFIED DRUG;

ALPHA CHAIN; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING SITE; BIOPHYSICS; CARBOXY TERMINAL SEQUENCE; CHO CELL; CONTROLLED STUDY; DRUG INHIBITION; DRUG ISOLATION; IMMUNOPRECIPITATION; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SODIUM CURRENT; SPIDER;

EID: 33745167385     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.105.021147     Document Type: Article

References (31)

1. Araùjo DA, Cordeiro MN, Diniz CR, and Beirão PSL (1993) Effect of a toxic fraction, PhTx2, from the spider Phoneutria nigriventer on the sodium current. Naunyn-Schmiedeberg's Arch Pharmacol 347:205-208.
2. Barbier J, Benoit E, and Molgó J (2003) Conotoxins targeting voltage-sensitive Na+ channels, in Bacterial, Plant and Animal Toxins (Ascenti P, Polticelli F, and Visca P eds) pp 127-145, Research Signpost, India.
3. Bulaj G, West PJ, Garrett JE, Marsh M, Zhang MM, Norton RS, Smith BJ, Yoshikami D, and Olivera BM (2005) Novel conotoxins from Conus striatus and Conus kinoshitai selectively block TTX-resistant sodium channels. Biochemistry 44:7259-7265.
4. Cassola AC, Jaffe H, Fales HM, Castro-Afeche S, Magnoli F, and Cipolla-Neto J (1998) omega-Phonetoxin-IIA: a calcium channel blocker from the spider Phoneutria nigriventer. Pflueg Arch Eur J Physiol 436:545-552.
5. Catterall WA, Goldin AL, and Waxman SG (2003) International Union of Pharmacology. XXXIX. Compendium of voltage-gated ion channels: sodium channels. Pharmacol Rev 55:575-578.
6. Cestèle S and Catterall WA (2000) Molecular mechanisms of neurotoxin action on voltage-gated sodium channels. Biochimie (Paris) 82:883-892.
7. Cordeiro MN, de Figueiredo SG, Valentim AC, Diniz CR, von Eickstedt VRD, Gilroy J, and Richardson M (1993) Purification and amino acid sequences of six Tx3 type neurotoxins from the venom of the Brazilian 'armed' spider Phoneutria nigriventer (Keys). Toxicon 31:35-42.
8. Cruz LJ, Gray WR, Olivera BM, Zeikus RD, Kerr L, Yoshikami D, and Moczydlowski E (1985) Conus geographus toxins that discriminate between neuronal and muscle sodium channels. J Biol Chem 260:9280-9288.
9. + current inactivation in insect CNS via binding to receptor site 3. J Insect Physiol 48:53-61.
10. Diniz CR, Cordeiro MN, Rezende L Jr, Kelly P, Fischer S, Reimann F, Oliveira EB, and Richardson M (1990) The purification and amino acid sequence of the lethal neurotoxin Tx1 from the venom of the Brazilian 'armed' spider Phoneutria nigriventer. FEBS Lett 263:251-253.
11. Diniz MRV, Paine MJI, Diniz CR, Theakston RDG, and Crampton JM (1993) Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin. J Biol Chem 268:15340-15342.
12. Fontana MD and Vital-Brazil O (1985) Mode of action of Phoneutria nigriventer spider venom at the isolated phrenic nerve-diaphragm of the rat. Braz J Med Biol Res 18:557-565.
13. Gomez MV, Kalapothakis E, Guatimosim C, and Prado MA (2002) Phoneutria nigriventer venom: a cocktail of toxins that affect ion channels. Cell Mol Neurobiol 22:579-588.
14. Gouvêa dos Santos R, Diniz CR, Cordeiro MN, and De Lima ME (1999) Binding sites and actions of Tx1, a neurotoxin from the venom of the spider Phoneutria nigriventer, in guinea pig ileum. Braz J Med Biol Res 32:1565-1569.
15. v2 family of calcium channels and interacts with ω-conotoxin-binding sites. J Biol Chem 277:13856-13862.
16. Huang X and Miller W (1991) A time-efficient, linear-space local similarity algorithm. Adv Appl Math 12:337-357.
17. Leão RM, Cruz JS, Diniz CR, Cordeiro MN, and Beirão PS (2000) Inhibition of neuronal high-voltage activated calcium channels by the ω-Phoneutria nigriventer Tx3-3 peptide toxin. Neuropharmacology 39:1756-1767.
18. Li RA and Tomaselli GF (2004) Using the deadly mu-conotoxins as probes of voltage-gated sodium channels. Toxicon 44:117-122.
19. + channels at different holding potentials: fluctuation measurements in frog myelinated nerve. Biochim Biophys Acta 985:161-167.
20. Lönnendonker U (1989b) Use-dependent block of sodium channels in frog myelinated nerve by tetrodotoxin and saxitoxin at negative holding potentials. Biochim Biophys Acta 985:153-160.
21. Love S and Cruz-Höfling MA (1986) Acute swelling of nodes of Ranvier caused by venoms which slow inactivation of sodium channels. Acta Neuropathol (Berl) 70:1-9.
22. Martin-Moutot N, Lévêque C, Sato K, Kato R, Takahashi M, and Seagar M (1995) Properties of ω conotoxin MVIIC receptors associated with α1A calcium channel subunits in rat brain. FEBS Lett 366:21-25.
23. Matavel A, Cruz JS, Penaforte CL, Araùjo DAM, Kalapothakis E, Prado VF, Diniz CR, Cordeiro MN, and Beirão PSL (2002) Electrophysiological characterization and molecular identification of the Phoneutria nigriventer peptide toxin PnTx2-6. FEBS Lett 523:219-223.
24. 3H]saxitoxin and μ-conotoxins. Proc Natl Acad Sci USA 83:5321-5325.
25. Patton DE and Goldin AL (1991) A voltage-dependent gating transition induces use-dependent block by tetrodotoxin of rat IIA sodium channels expressed in Xenopus oocytes. Neuron 7:637-647.
26. Peng K, Shu Q, Zhonghua L, and Liang S (2002) Function and solution structure of Huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena. J Biol Chem 277:47564-47571.
27. Sarkar SN, Adhikari A, and Sikdar SK (1995) Kinetic characterization of rat brain type IIA sodium channel alpha-subunit stably expressed in a somatic cell line. J Physiol (Lond) 488:633-645.
28. Sato K, Ishida Y, Wakamatsu K, Kato R, Honda H, Ohizumi Y, Nakamura H, Ohya M, Lancelin J-M, Kohda D, et al. (1991) Active site of μ-conotoxin GIIIA, a peptide blocker of muscle sodium channels. J Biol Chem 266:16989-16991.
29. Terlau H and Olivera BM (2004) Conus venoms: a rich source of novel ion channel-targeted peptides. Physiol Rev 84:41-68.
30. Vieira LB, Kushmerick C, Hildebrand ME, Garcia E, Stea A, Cordeiro MN, Richardson M, Gomez MV, and Snutch TP (2005) Inhibition of high voltage-activated calcium channels by spider toxin PnTx3-6. J Pharmacol Exp Ther 314:1370-1377.
31. + channel α subunits in a somatic cell line. Neuron 8:59-70.