메뉴 건너뛰기




Volumn 17, Issue 8, 2009, Pages 353-365

Identification of endogenous reference genes for qRT-PCR analysis in normal matched breast tumor tissues

Author keywords

Breast cancer; Endogenous reference genes; Normalization factor; Real time quantitative RT PCR

Indexed keywords

TUMOR MARKER;

EID: 67650318530     PISSN: 09650407     EISSN: None     Source Type: Journal    
DOI: 10.3727/096504009788428460     Document Type: Article
Times cited : (52)

References (52)
  • 1
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
    • Bustin, S. A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J. Mol. Endocrinol. 29:23-39; 2002.
    • (2002) J. Mol. Endocrinol. , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 2
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl, M. W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45; 2001.
    • (2001) Nucleic Acids Res. , vol.29
    • Pfaffl, M.W.1
  • 3
    • 1842829051 scopus 로고    scopus 로고
    • Real- Time RT-PCR: Considerations for efficient and sensitive assay design
    • Peters, I. R.; Helps, C. R.; Hall, E. J.; Day, M. J. Real- time RT-PCR: Considerations for efficient and sensitive assay design. J. Immunol. Methods 286:203-217; 2004.
    • (2004) J. Immunol. Methods , vol.286 , pp. 203-217
    • Peters, I.R.1    Helps, C.R.2    Hall, E.J.3    Day, M.J.4
  • 4
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    • Bustin, S. A.; Nolan, T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15:155-166; 2004.
    • (2004) J. Biomol. Tech. , vol.15 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 6
    • 4344564046 scopus 로고    scopus 로고
    • Comparison of reverse transcriptases in gene expression analysis
    • Stahlberg, A.; Kubista, M.; Pfaffl, M. Comparison of reverse transcriptases in gene expression analysis. Clin. Chem. 50:1678-1680; 2004.
    • (2004) Clin. Chem. , vol.50 , pp. 1678-1680
    • Stahlberg, A.1    Kubista, M.2    Pfaffl, M.3
  • 7
    • 1542328972 scopus 로고    scopus 로고
    • Properties of the reverse transcription reaction in mRNA quantification
    • Stahlberg, A.; Hakansson, J.; Xian, X.; Semb, H.; Kubista, M. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50:509-515; 2004.
    • (2004) Clin. Chem. , vol.50 , pp. 509-515
    • Stahlberg, A.1    Hakansson, J.2    Xian, X.3    Semb, H.4    Kubista, M.5
  • 8
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method
    • Livak, K. J.; Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 24:402-408; 2001.
    • (2001) Methods , vol.24 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 9
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele, J.; De Preter, K.; Pattyn, F.; Poppe, B.; Van Roy, N.; De Paepe, A.; Speleman, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:0034.1-0034.11; 2002.
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 10
    • 0034694128 scopus 로고    scopus 로고
    • Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR
    • Schmittgen, T. D.; Zakrajsek, B. A. Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Methods 46:69-81; 2000.
    • (2000) J. Biochem. Biophys. Methods , vol.46 , pp. 69-81
    • Schmittgen, T.D.1    Zakrajsek, B.A.2
  • 11
    • 0033575003 scopus 로고    scopus 로고
    • Direct comparison of GAPDH, α-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
    • Zhong, H.; Simons, J. W. Direct comparison of GAPDH, α-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem. Biophys. Res. Commun. 259:523-526; 1999.
    • (1999) Biochem. Biophys. Res. Commun. , vol.259 , pp. 523-526
    • Zhong, H.1    Simons, J.W.2
  • 12
    • 21244499405 scopus 로고    scopus 로고
    • GAPDH as a housekeeping gene: Analysis of GAPDH mRNA expression in a panel of 72 human tissues
    • Barber, R. D.; Harmer, D. W.; Coleman, R. A.; Clark, B. J. GAPDH as a housekeeping gene: Analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol. Genom. 21:389-395; 2005.
    • (2005) Physiol. Genom. , vol.21 , pp. 389-395
    • Barber, R.D.1    Harmer, D.W.2    Coleman, R.A.3    Clark, B.J.4
  • 14
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single house- Keeping genes is inappropriate for human tissue biopsies
    • Tricarico, C.; Pinzani, P.; Bianchi, S.; Paglierani, M.; Distante, V.; Pazzagli, M.; Bustin, S. A.; Orlando, C. Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single house- keeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309:293-300; 2002.
    • (2002) Anal. Biochem. , vol.309 , pp. 293-300
    • Tricarico, C.1    Pinzani, P.2    Bianchi, S.3    Paglierani, M.4    Distante, V.5    Pazzagli, M.6    Bustin, S.A.7    Orlando, C.8
  • 15
    • 0034091196 scopus 로고    scopus 로고
    • A reliability test of standard-based quantitative PCR: Exogenous vs endogenous standards
    • Ke, L. D.; Chen, Z.; Yung, W. K. A reliability test of standard-based quantitative PCR: exogenous vs endogenous standards. Mol. Cell Probes 14:127-135; 2000.
    • (2000) Mol. Cell Probes , vol.14 , pp. 127-135
    • Ke, L.D.1    Chen, Z.2    Yung, W.K.3
  • 17
    • 13944276352 scopus 로고    scopus 로고
    • Clinicopathologic correlation of up-regulated genes identified using cDNA microarray and real-time reverse transcription-PCR in human colorectal cancer
    • Chiu, S. T.; Hsieh, F. J.; Chen, S. W.; Chen, C. L.; Shu, H. F.; Li, H. Clinicopathologic correlation of up-regulated genes identified using cDNA microarray and real-time reverse transcription-PCR in human colorectal cancer. Cancer Epidemiol. Biomarkers Prev. 14:437-443; 2005.
    • (2005) Cancer Epidemiol. Biomarkers Prev. , vol.14 , pp. 437-443
    • Chiu, S.T.1    Hsieh, F.J.2    Chen, S.W.3    Chen, C.L.4    Shu, H.F.5    Li, H.6
  • 18
    • 34548028445 scopus 로고    scopus 로고
    • Molecular profiling of matched samples identifies biomarkers of papillary thyroid carcinoma lymph node metastasis
    • Cerutti, J. M.; Oler, G.; Michaluart, Jr., P.; Delcelo, R.; Beaty, R. M.; Shoemaker, J.; Riggins, G. J. Molecular profiling of matched samples identifies biomarkers of papillary thyroid carcinoma lymph node metastasis. Cancer Res. 67:7885-7892; 2007.
    • (2007) Cancer Res. , vol.67 , pp. 7885-7892
    • Cerutti, J.M.1    Oler, G.2    Michaluart Jr., P.3    Delcelo, R.4    Beaty, R.M.5    Shoemaker, J.6    Riggins, G.J.7
  • 19
    • 28244493099 scopus 로고    scopus 로고
    • Distinct mRNA, protein expression patterns and distribution of oestrogen receptors alpha and beta in human primary breast cancer: Correlation with proliferation marker Ki-67 and clinicopathological factors
    • DOI 10.1016/j.ejca.2005.09.010, PII S0959804905008014
    • Jarzabek, K.; Koda, M.; Kozlowski, L.; Mittre, H.; Sulkowski, S.; Kottler, M. L.; Wolczynski, S. Distinct mRNA, protein expression patterns and distribution of oestrogen receptors alpha and beta in human primary breast cancer: Correlation with proliferation marker Ki-67 and clinicopathological factors. Eur. J. Cancer 41:2924-2934; 2005. (Pubitemid 41713133)
    • (2005) European Journal of Cancer , vol.41 , Issue.18 , pp. 2924-2934
    • Jarzabek, K.1    Koda, M.2    Kozlowski, L.3    Mittre, H.4    Sulkowski, S.5    Kottler, M.-L.6    Wolczynski, S.7
  • 20
    • 33646504879 scopus 로고    scopus 로고
    • Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression
    • Chao, A.; Wang, T. H.; Lee, Y. S.; Hsueh, S.; Chao, A. S.; Chang, T. C.; Kung, W. H.; Huang, S. L.; Chao, F. Y.; Wei, M. L.; Lai, C. H. Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression. Int. J. Cancer 119:91-98; 2006.
    • (2006) Int. J. Cancer , vol.119 , pp. 91-98
    • Chao, A.1    Wang, T.H.2    Lee, Y.S.3    Hsueh, S.4    Chao, A.S.5    Chang, T.C.6    Kung, W.H.7    Huang, S.L.8    Chao, F.Y.9    Wei, M.L.10    Lai, C.H.11
  • 23
    • 33646002325 scopus 로고    scopus 로고
    • Identification and validation of suitable endogenous reference genes for gene expression studies of human bladder cancer
    • Ohl, F.; Jung, M.; Radonic, A.; Sachs, M.; Loening, S. A.; Jung, K. Identification and validation of suitable endogenous reference genes for gene expression studies of human bladder cancer. J. Urol. 175:1915-1920; 2006.
    • (2006) J. Urol. , vol.175 , pp. 1915-1920
    • Ohl, F.1    Jung, M.2    Radonic, A.3    Sachs, M.4    Loening, S.A.5    Jung, K.6
  • 25
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen, C. L.; Jensen, J. L.; Orntoft, T. F. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64:5245-5250; 2004.
    • (2004) Cancer Res. , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 26
    • 14644429677 scopus 로고    scopus 로고
    • Reliable transcript quantification by real-time reverse transcriptase-polymerase chain reaction in primary neuroblastoma using normalization to averaged expression levels of the control genes HPRT1 and SDHA
    • Fischer, M.; Skowron, M.; Berthold, F. Reliable transcript quantification by real-time reverse transcriptase-polymerase chain reaction in primary neuroblastoma using normalization to averaged expression levels of the control genes HPRT1 and SDHA. J. Mol. Diagn. 7:89-96; 2005.
    • (2005) J. Mol. Diagn. , vol.7 , pp. 89-96
    • Fischer, M.1    Skowron, M.2    Berthold, F.3
  • 27
    • 33748198211 scopus 로고    scopus 로고
    • Selection of suitable reference genes for accurate normalization of gene expression profile studies in non-small cell lung cancer
    • Saviozzi, S.; Cordero, F.; Lo,acono, M.; Novello, S.; Scagliotti, G. V.; Calogero, R. A. Selection of suitable reference genes for accurate normalization of gene expression profile studies in non-small cell lung cancer. BMC Cancer 6:200-209; 2006.
    • (2006) BMC Cancer , vol.6 , pp. 200-209
    • Saviozzi, S.1    Cordero, F.2    Loacono, M.3    Novello, S.4    Scagliotti, G.V.5    Calogero, R.A.6
  • 28
    • 29244459423 scopus 로고    scopus 로고
    • Choice of endogenous control for gene expression in nonsmall cell lung cancer
    • Liu, D. W.; Chen, S. T.; Liu, H. P. Choice of endogenous control for gene expression in nonsmall cell lung cancer. Eur. Respir. J. 26:1002-1008; 2005.
    • (2005) Eur. Respir. J. , vol.26 , pp. 1002-1008
    • Liu, D.W.1    Chen, S.T.2    Liu, H.P.3
  • 31
    • 33748971354 scopus 로고    scopus 로고
    • Reduced vascular endothelial growth inhibitor (VEGI) expression is associated with poor prognosis in breast cancer patients
    • Parr, C.; Gan, C. H.; Watkins, G.; Jiang, W. G. Reduced vascular endothelial growth inhibitor (VEGI) expression is associated with poor prognosis in breast cancer patients. Angiogenesis 9:73-81; 2006.
    • (2006) Angiogenesis , vol.9 , pp. 73-81
    • Parr, C.1    Gan, C.H.2    Watkins, G.3    Jiang, W.G.4
  • 33
    • 33744994778 scopus 로고    scopus 로고
    • Lower expression of CXCR4 in lymph node metastases than in primary breast cancers: Potential regulation by ligand-dependent degradation and HIF-1alpha
    • Shim, H.; Lau, S. K.; Devi, S.; Yoon, Y.; Cho, H. T.; Liang, Z. Lower expression of CXCR4 in lymph node metastases than in primary breast cancers: Potential regulation by ligand-dependent degradation and HIF-1alpha. Biochem. Biophys. Res. Commun. 346:252-258; 2006.
    • (2006) Biochem. Biophys. Res. Commun. , vol.346 , pp. 252-258
    • Shim, H.1    Lau, S.K.2    Devi, S.3    Yoon, Y.4    Cho, H.T.5    Liang, Z.6
  • 34
    • 20444368021 scopus 로고    scopus 로고
    • Determining suitable internal standards for mRNA quantification of increasing cancer progression in human breast cells by real-time reverse transcriptase polymerase chain reaction
    • Morse, D. L.; Carroll, D.; Weberg, L.; Borgstrom, M. C.; Ranger-Moore, J.; Gillies, R. J. Determining suitable internal standards for mRNA quantification of increasing cancer progression in human breast cells by real-time reverse transcriptase polymerase chain reaction. Anal. Biochem. 342:69-77; 2005.
    • (2005) Anal. Biochem. , vol.342 , pp. 69-77
    • Morse, D.L.1    Carroll, D.2    Weberg, L.3    Borgstrom, M.C.4    Ranger-Moore, J.5    Gillies, R.J.6
  • 38
    • 0034668114 scopus 로고    scopus 로고
    • Quantitative analysis of estrogen receptor-alpha and-beta messenger RNA expression in breast carcinoma by real-time polymerase chain reaction
    • DOI 10.1002/1097-0142(20001015)89:8<1732::AID-CNCR13>3.0.CO;2-2
    • Iwao, K.; Miyoshi, Y.; Egawa, C.; Ikeda, N.; Tsukamoto, F.; Noguchi, S. Quantitative analysis of estrogen receptor- alpha and -beta messenger RNA expression in breast carcinoma by real-time polymerase chain reaction. Cancer 89:1732-1738; 2000. (Pubitemid 30794167)
    • (2000) Cancer , vol.89 , Issue.8 , pp. 1732-1738
    • Iwao, K.1    Miyoshi, Y.2    Egawa, C.3    Ikeda, N.4    Tsukamoto, F.5    Noguchi, S.6
  • 41
    • 38349106695 scopus 로고    scopus 로고
    • Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer
    • McNeill, R. E.; Miller, N.; Kerin, M. J. Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer. BMC Mol. Biol. 8:107; 2007.
    • (2007) BMC Mol. Biol. , vol.8 , pp. 107
    • McNeill, R.E.1    Miller, N.2    Kerin, M.J.3
  • 42
    • 40549111703 scopus 로고    scopus 로고
    • Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
    • Lyng, M. B.; Laenkholm, A. V.; Pallisgaard, N.; Ditzel, H. J. Identification of genes for normalization of real-time RT-PCR data in breast carcinomas. BMC Cancer 8:20; 2008.
    • (2008) BMC Cancer , vol.8 , pp. 20
    • Lyng, M.B.1    Laenkholm, A.V.2    Pallisgaard, N.3    Ditzel, H.J.4
  • 45
    • 0002129975 scopus 로고    scopus 로고
    • Quantification on the LightCycler
    • Meuer, S.; Witter, C.; Nakagawara, K., eds. Berlin: Springer
    • Rasmussen, R. Quantification on the LightCycler. In: Meuer, S.; Witter, C.; Nakagawara, K., eds. Rapid cycle real-time PCR, methods and applications. Berlin: Springer; 2001:21-34.
    • (2001) Rapid Cycle Real-time PCR, Methods and Applications , pp. 21-34
    • Rasmussen, R.1
  • 47
    • 0036353085 scopus 로고    scopus 로고
    • Molecular mechanism of transcriptional repression of gelsolin in human breast cancer cells
    • Dong, Y.; Asch, H. L.; Ying, A.; Asch, B. B. Molecular mechanism of transcriptional repression of gelsolin in human breast cancer cells. Exp. Cell Res. 276:328-336; 2002.
    • (2002) Exp. Cell Res. , vol.276 , pp. 328-336
    • Dong, Y.1    Asch, H.L.2    Ying, A.3    Asch, B.B.4
  • 49
    • 33344475791 scopus 로고    scopus 로고
    • Limitations of commonly used internal controls for real-time RT-PCR analysis of renal epithelial-mesenchymal cell transition
    • Elberg, G.; Elberg, D.; Logan, C. J.; Chen, L.; Turman, M. A. Limitations of commonly used internal controls for real-time RT-PCR analysis of renal epithelial-mesenchymal cell transition. Nephron Exp. Nephrol. 102:113-122; 2006.
    • (2006) Nephron Exp. Nephrol. , vol.102 , pp. 113-122
    • Elberg, G.1    Elberg, D.2    Logan, C.J.3    Chen, L.4    Turman, M.A.5
  • 50
    • 0027218078 scopus 로고
    • Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls
    • Spanakis, E. Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls. Nucleic Acids Res. 21: 3809-3819; 1993. (Pubitemid 23254504)
    • (1993) Nucleic Acids Research , vol.21 , Issue.16 , pp. 3809-3819
    • Spanakis, E.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.