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Volumn 43, Issue 2, 2009, Pages 97-107

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Author keywords

Alkaline comet assay; Diffusion test; Fpg modified comet assay; Gamma radiation; Human peripheral blood lymphocytes; ROS

Indexed keywords

DNA FORMAMIDOPYRIMIDINE GLYCOSYLASE;

EID: 67650309046     PISSN: 13182099     EISSN: 15813207     Source Type: Journal    
DOI: 10.2478/v10019-009-0015-y     Document Type: Article
Times cited : (25)

References (50)
  • 1
    • 0037118277 scopus 로고    scopus 로고
    • Major radiation exposurewhat to expect and how to respond
    • Mettler FA, Voelz GL. Major radiation exposurewhat to expect and how to respond. N Engl J Med 2002; 346: 1554-61.
    • (2002) N Engl J Med , vol.346 , pp. 1554-1561
    • Mettler, F.A.1    Voelz, G.L.2
  • 3
    • 58149218742 scopus 로고    scopus 로고
    • Oxidative damage to DNA in non-malignant disease: Biomarker or biohazard?
    • Cooke M, Evans M. Oxidative damage to DNA in non-malignant disease: biomarker or biohazard? Genome Dyn 2006; 1: 53-66.
    • (2006) Genome Dyn , vol.1 , pp. 53-66
    • Cooke, M.1    Evans, M.2
  • 4
    • 33845969499 scopus 로고    scopus 로고
    • Regulation of reactive oxygen species by Atm is essential for proper response to DNA double-strand breaks in lymphocytes
    • Ito K, Takubo K, Satoh H, Matsuoka S, Ohmura M, Naka K et al. Regulation of reactive oxygen species by Atm is essential for proper response to DNA double-strand breaks in lymphocytes. J Immunol 2007; 178: 103-10.
    • (2007) J Immunol , vol.178 , pp. 103-110
    • Ito, K.1    Takubo, K.2    Satoh, H.3    Matsuoka, S.4    Ohmura, M.5    Naka, K.6
  • 5
    • 0642375804 scopus 로고    scopus 로고
    • Chromosomal DNA Fragmentation in apoptosis and necrosis induced by oxidative stress
    • Higuchi Y. Chromosomal DNA Fragmentation in apoptosis and necrosis induced by oxidative stress. Biochem Pharmacol 2003; 66: 1527-35.
    • (2003) Biochem Pharmacol , vol.66 , pp. 1527-1535
    • Higuchi, Y.1
  • 7
    • 52049122167 scopus 로고    scopus 로고
    • The micronucleus assay determination of chromosomal level DNA damage
    • Fenech M. The micronucleus assay determination of chromosomal level DNA damage. Methods Mol Biol 2008; 410: 185-216.
    • (2008) Methods Mol Biol , vol.410 , pp. 185-216
    • Fenech, M.1
  • 8
    • 33846684170 scopus 로고    scopus 로고
    • Molecular mechanisms of sister-chromatid exchange
    • Willson DM, Thompson LH. Molecular mechanisms of sister-chromatid exchange. Mutat Res 2006; 616: 11-23.
    • (2006) Mutat Res , vol.616 , pp. 11-23
    • Willson, D.M.1    Thompson, L.H.2
  • 9
    • 33751119273 scopus 로고    scopus 로고
    • Chromosomal changes: Induction, detection methods and applicability in human biomonitoring
    • Mateuca R, Lombaert N, Aka PV, Decodier I, Kirsch-Volders M. Chromosomal changes: induction, detection methods and applicability in human biomonitoring. Biochimie 2006; 88: 1515-31.
    • (2006) Biochimie , vol.88 , pp. 1515-1531
    • Mateuca, R.1    Lombaert, N.2    Aka, P.V.3    Decodier, I.4    Kirsch-Volders, M.5
  • 10
    • 30844440734 scopus 로고    scopus 로고
    • Usefulness and limits of biological dosimetry based on cytogenetic methods
    • Leonard A, Rueff J, Gerber GB, Leonard ED. Usefulness and limits of biological dosimetry based on cytogenetic methods. Radiat Prot Dosimetry 2005; 115: 448-54.
    • (2005) Radiat Prot Dosimetry , vol.115 , pp. 448-454
    • Leonard, A.1    Rueff, J.2    Gerber, G.B.3    Leonard, E.D.4
  • 11
    • 0342904908 scopus 로고    scopus 로고
    • Comet assay in genetic ecotoxicology: A review
    • Cotelle S, Ferrard JF. Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 1999; 34: 246-55.
    • (1999) Environ Mol Mutagen , vol.34 , pp. 246-255
    • Cotelle, S.1    Ferrard, J.F.2
  • 12
    • 0034630349 scopus 로고    scopus 로고
    • A simple method for accurate estimation of apoptotic cells
    • Singh NP. A simple method for accurate estimation of apoptotic cells. Exp Cell Res 2000; 256: 328-37.
    • (2000) Exp Cell Res , vol.256 , pp. 328-337
    • Singh, N.P.1
  • 13
    • 33645739658 scopus 로고    scopus 로고
    • The alkaline comet assay: Towards validation in biomonitoring of DNA damaging exposures
    • Møller P. The alkaline comet assay: towards validation in biomonitoring of DNA damaging exposures. Basic Clin Pharmacol Toxicol 2006; 98 : 336-45.
    • (2006) Basic Clin Pharmacol Toxicol , vol.98 , pp. 336-345
    • Møller, P.1
  • 14
    • 0023919963 scopus 로고
    • A simple technique for quantitation of low levels of DNA damage in individual cells
    • Singh NP, McCoy MT, Tice RR, Schneider LL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-91.
    • (1988) Exp Cell Res , vol.175 , pp. 184-191
    • Singh, N.P.1    McCoy, M.T.2    Tice, R.R.3    Schneider, L.L.4
  • 15
    • 0026005914 scopus 로고    scopus 로고
    • Tice RR, Andrews PW, Hirai O, Singh NP. The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. In Witmer CM, Snyder RR, Hollow DJ, Kalf GF, Kocsis JJ, Sipes JG, editor. Biological Reactive Intermediates. IV. Molecular and Cellular Effects and their Impact on Human Health. New York: Plenum Press; 1990. p. 157-64.
    • Tice RR, Andrews PW, Hirai O, Singh NP. The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. In Witmer CM, Snyder RR, Hollow DJ, Kalf GF, Kocsis JJ, Sipes JG, editor. Biological Reactive Intermediates. IV. Molecular and Cellular Effects and their Impact on Human Health. New York: Plenum Press; 1990. p. 157-64.
  • 17
    • 0032959983 scopus 로고    scopus 로고
    • DNA damage and repair in individual cells: Applications of the comet assay in radiobiology
    • Olive PL. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Radiat Biol 1999; 75: 395-405.
    • (1999) Int J Radiat Biol , vol.75 , pp. 395-405
    • Olive, P.L.1
  • 18
    • 0035990239 scopus 로고    scopus 로고
    • Application of the alkaline comet assay in biodosimetry: Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident
    • Garaj-Vrhovac V, Kopjar N, Ražem D, Vekić B, Miljanic S, Ranogajec-Komor M. Application of the alkaline comet assay in biodosimetry: assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident. Radiat Prot Dosimetry 2002; 98(4): 407-16.
    • (2002) Radiat Prot Dosimetry , vol.98 , Issue.4 , pp. 407-416
    • Garaj-Vrhovac, V.1    Kopjar, N.2    Ražem, D.3    Vekić, B.4    Miljanic, S.5    Ranogajec-Komor, M.6
  • 19
    • 48549084307 scopus 로고    scopus 로고
    • Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay
    • Gajski G, Garaj-Vrhovac V, Orescanin V. Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay. Toxicol Appl Pharmacol 2008; 231: 85-93.
    • (2008) Toxicol Appl Pharmacol , vol.231 , pp. 85-93
    • Gajski, G.1    Garaj-Vrhovac, V.2    Orescanin, V.3
  • 21
    • 8544283254 scopus 로고    scopus 로고
    • The comet assay for DNA damage and repair: Principles, applications, and limitations
    • Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 2004; 26: 249-62.
    • (2004) Mol Biotechnol , vol.26 , pp. 249-262
    • Collins, A.R.1
  • 22
    • 30644457199 scopus 로고    scopus 로고
    • The comet assay to determine the mode of cell death for the ultrasonic delivery of doxorubicin to human leukemia (HL-60 Cells) from Pluronic P105 micelles
    • Husseini GA, O'Neill KL, Pitt WG. The comet assay to determine the mode of cell death for the ultrasonic delivery of doxorubicin to human leukemia (HL-60 Cells) from Pluronic P105 micelles. Technol Cancer Res Treat 2005; 4: 707-99.
    • (2005) Technol Cancer Res Treat , vol.4 , pp. 707-799
    • Husseini, G.A.1    O'Neill, K.L.2    Pitt, W.G.3
  • 23
    • 0027375157 scopus 로고
    • Direct enzymatic detection of endogenous oxidative base damage in human lymphocyte DNA
    • Collins AR, Duthie SJ, Dobson VL. Direct enzymatic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 1993; 14 : 1733-5.
    • (1993) Carcinogenesis , vol.14 , pp. 1733-1735
    • Collins, A.R.1    Duthie, S.J.2    Dobson, V.L.3
  • 24
    • 0030861915 scopus 로고    scopus 로고
    • DNA glycosylases in the base excision repair
    • Krokan HE, Standal R, Slupphaug G. DNA glycosylases in the base excision repair. Biochem J 1997; 325: 1-16.
    • (1997) Biochem J , vol.325 , pp. 1-16
    • Krokan, H.E.1    Standal, R.2    Slupphaug, G.3
  • 25
    • 0345276655 scopus 로고    scopus 로고
    • Sensitivity of the FPG protein towards alkylation damage in the comet assay
    • Speit G, Schütz P, Bonzheim I, Trenz K, Hoffman H. Sensitivity of the FPG protein towards alkylation damage in the comet assay. Toxicol Lett 2003; 146: 151-8.
    • (2003) Toxicol Lett , vol.146 , pp. 151-158
    • Speit, G.1    Schütz, P.2    Bonzheim, I.3    Trenz, K.4    Hoffman, H.5
  • 26
    • 20544432769 scopus 로고    scopus 로고
    • Apoptosis Assessment by the DNA diffusion assay
    • Singh NP. Apoptosis Assessment by the DNA diffusion assay. Methods Mol Med 2005; 111: 55-67.
    • (2005) Methods Mol Med , vol.111 , pp. 55-67
    • Singh, N.P.1
  • 28
    • 0002230857 scopus 로고
    • Morphological and biochemical assays of apoptosis
    • Coligan JE, Kruisbeal AM, editor, New York: John Willey & Sons;
    • Duke RC, Cohen JJ. Morphological and biochemical assays of apoptosis. In: Coligan JE, Kruisbeal AM, editor. Current Protocols in Immunology. New York: John Willey & Sons; 1992. p. 1-3.
    • (1992) Current Protocols in Immunology , pp. 1-3
    • Duke, R.C.1    Cohen, J.J.2
  • 29
    • 0034693836 scopus 로고    scopus 로고
    • DNA protein crosslinks and apoptosis
    • for estimation of DNA strand breaks
    • Singh NP. Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat Res 2000; 455: 111-27.
    • (2000) Mutat Res , vol.455 , pp. 111-127
    • Microgels, S.N.P.1
  • 30
    • 84927972687 scopus 로고    scopus 로고
    • Comet Assay interest group website
    • Comet Assay interest group website: http://cometassay.com/
  • 31
    • 33745202038 scopus 로고    scopus 로고
    • Apoptosis by DNA diffusion assay
    • Blumenthal R, editor, New York: Humana Press;
    • Singh NP. Apoptosis by DNA diffusion assay. In: Blumenthal R, editor. Methods in Molecular Medicine-Chemosensitivity, New York: Humana Press; 2003. p. 78-94.
    • (2003) Methods in Molecular Medicine-Chemosensitivity , pp. 78-94
    • Singh, N.P.1
  • 32
    • 0031973021 scopus 로고    scopus 로고
    • The ability of the Comet assay to discriminate between genotoxins and cytotoxins
    • Handerson L, Wolfreys A, Fedyk J, Bourner C, Windebank S. The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 1998; 13: 89-94.
    • (1998) Mutagenesis , vol.13 , pp. 89-94
    • Handerson, L.1    Wolfreys, A.2    Fedyk, J.3    Bourner, C.4    Windebank, S.5
  • 35
    • 33645857360 scopus 로고    scopus 로고
    • Phytohaemagglutinin as a modulator of DNA repair measured by chromosome aberration analysis in micronucleus assay in ionizing radiation biodosimetry
    • Durinec M, Želježić D, Garaj-Vrhovac V. Phytohaemagglutinin as a modulator of DNA repair measured by chromosome aberration analysis in micronucleus assay in ionizing radiation biodosimetry. Radiol Oncol 2006; 40: 43-9.
    • (2006) Radiol Oncol , vol.40 , pp. 43-49
    • Durinec, M.1    Želježić, D.2    Garaj-Vrhovac, V.3
  • 36
    • 1942502411 scopus 로고    scopus 로고
    • Comet assay in the assessment of the human genome damage induced by -radiation in vitro
    • Garaj-Vrhovac V, Želježić D. Comet assay in the assessment of the human genome damage induced by -radiation in vitro. Radiol Oncol 2004; 38: 43-7.
    • (2004) Radiol Oncol , vol.38 , pp. 43-47
    • Garaj-Vrhovac, V.1    Želježić, D.2
  • 37
    • 33750065724 scopus 로고    scopus 로고
    • Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells
    • Sudprasert W, Navasumrit P, Ruchirawat M. Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells. Int J Hyg Environ Health 2006; 209: 503-11.
    • (2006) Int J Hyg Environ Health , vol.209 , pp. 503-511
    • Sudprasert, W.1    Navasumrit, P.2    Ruchirawat, M.3
  • 39
    • 63449140856 scopus 로고    scopus 로고
    • Evaluation of basal DNA damage and oxidative stress in Wistar rat leukocytes after exposure to microwave radiation
    • Garaj-Vrhovac V, Gajski G, Trošić I, Pavičić I. Evaluation of basal DNA damage and oxidative stress in Wistar rat leukocytes after exposure to microwave radiation Toxicology 2009; 259: 17-12.
    • (2009) Toxicology , vol.259 , pp. 17-12
    • Garaj-Vrhovac, V.1    Gajski, G.2    Trošić, I.3    Pavičić, I.4
  • 40
    • 33751022467 scopus 로고    scopus 로고
    • DNA damage and repair capacity by comet assay in lymhocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace
    • Fracasso ME, Doria D, Franceschetti P, Perbellini L, Romeo L. DNA damage and repair capacity by comet assay in lymhocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace. Toxicol Lett 2006; 167: 131-41.
    • (2006) Toxicol Lett , vol.167 , pp. 131-141
    • Fracasso, M.E.1    Doria, D.2    Franceschetti, P.3    Perbellini, L.4    Romeo, L.5
  • 41
    • 34347398701 scopus 로고    scopus 로고
    • The sensitivity of the alkaline comet assay in detecting DNA lesions induced by x-rays, gamma rays and alpha particles
    • Rössler U, Hornhardt S, Seidl C, Müller-Laue E, Walsh L, Panzer W et al. The sensitivity of the alkaline comet assay in detecting DNA lesions induced by x-rays, gamma rays and alpha particles. Radiat Prot Dosimetry 2006; 122: 154-9.
    • (2006) Radiat Prot Dosimetry , vol.122 , pp. 154-159
    • Rössler, U.1    Hornhardt, S.2    Seidl, C.3    Müller-Laue, E.4    Walsh, L.5    Panzer, W.6
  • 42
    • 0002987035 scopus 로고
    • The single cell gel/comet assay: A microgel electrophoresis technique for the detection of DNA damage and repair in individual cells
    • Philips DH, Venitt S, editor, Oxford, UK: Bios Scientific Publishers;
    • Tice RR. The single cell gel/comet assay: a microgel electrophoresis technique for the detection of DNA damage and repair in individual cells. In: Philips DH, Venitt S, editor. Environmental mutagenesis. Oxford, UK: Bios Scientific Publishers; 1995. p. 315-39.
    • (1995) Environmental mutagenesis , pp. 315-339
    • Tice, R.R.1
  • 44
    • 0031922151 scopus 로고    scopus 로고
    • Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation
    • Malyapa RS, Bi C, Ahern EW, Roti Roti JL. Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation. Radiat Res 1998; 149: 396-400.
    • (1998) Radiat Res , vol.149 , pp. 396-400
    • Malyapa, R.S.1    Bi, C.2    Ahern, E.W.3    Roti Roti, J.L.4
  • 45
    • 2842526228 scopus 로고    scopus 로고
    • Detection of oxidised purines and UV-induced photoproducts in DNA of single cells, by inclusion of lesion specific enzymes in the comet assay
    • Collins A, Dusinská M. Detection of oxidised purines and UV-induced photoproducts in DNA of single cells, by inclusion of lesion specific enzymes in the comet assay. Altern Lab Anim 1996; 24: 405-11.
    • (1996) Altern Lab Anim , vol.24 , pp. 405-411
    • Collins, A.1    Dusinská, M.2
  • 47
    • 0027172322 scopus 로고    scopus 로고
    • Molecular regulation of apoptosis: Genetic control on cell death
    • Smith CA, Williams GT. Molecular regulation of apoptosis: genetic control on cell death. Cel. 2006; 74 : 777-9.
    • (2006) Cel , vol.74 , pp. 777-779
    • Smith, C.A.1    Williams, G.T.2
  • 48
    • 0029952976 scopus 로고    scopus 로고
    • Detection of DNA damage after hyperbaric oxygen (HBO) therapy
    • Dennog C, Hartmann A, Frey G, Speit G. Detection of DNA damage after hyperbaric oxygen (HBO) therapy. Mutagenesis 1996; 11: 605-9.
    • (1996) Mutagenesis , vol.11 , pp. 605-609
    • Dennog, C.1    Hartmann, A.2    Frey, G.3    Speit, G.4
  • 49
    • 23644452512 scopus 로고    scopus 로고
    • Evaluation of the nuclear DNA diffusion assay to detect apoptosis and necrosis
    • Gichner T, Mukherjee A, Wagner E, Plewa MJ. Evaluation of the nuclear DNA diffusion assay to detect apoptosis and necrosis. Mutat Res 2005; 586: 38-46
    • (2005) Mutat Res , vol.586 , pp. 38-46
    • Gichner, T.1    Mukherjee, A.2    Wagner, E.3    Plewa, M.J.4
  • 50
    • 1542347564 scopus 로고    scopus 로고
    • Cell membrane oxidative damage induced by gamma-radiation and apoptotic sensitivity
    • Mishra KP. Cell membrane oxidative damage induced by gamma-radiation and apoptotic sensitivity. J Environ Pathol Toxicol Oncol 2004; 23: 61-6.
    • (2004) J Environ Pathol Toxicol Oncol , vol.23 , pp. 61-66
    • Mishra, K.P.1


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