Label-free determination of protein-ligand binding constants using mass spectrometry and validation using surface plasmon resonance and isothermal titration calorimetry

Journal of Molecular Recognition

Volumn 22, Issue 4, 2009, Pages 319-329

  Jecklin, Matthias C ^a   Schauer, Stefan ^b   Dumelin, Christoph E ^a   Zenobi, Renato ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Carbonic anhydrase; Isothermal titration calorimetry; Nanoelectrospray mass spectrometry; Noncovalent complexes; Surface plasmon resonance

Indexed keywords

CARBONATE DEHYDRATASE I; ETHOXZOLAMIDE; LIGAND;

ARTICLE; CALORIMETRY; ELECTROSPRAY MASS SPECTROMETRY; HUMAN; KINETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PROTEIN BINDING; REPRODUCIBILITY; STAINING; SURFACE PLASMON RESONANCE; TIME; TITRIMETRY; VALIDATION STUDY;

CALORIMETRY; CARBONIC ANHYDRASE I; ETHOXZOLAMIDE; HUMANS; KINETICS; LIGANDS; MASS SPECTROMETRY; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STAINING AND LABELING; SURFACE PLASMON RESONANCE; TIME FACTORS; TITRIMETRY;

EID: 67650264161     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.951     Document Type: Article

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