Interspecies difference in liver-specific functions and biotransformation of testosterone of primary rat, porcine and human hepatocyte in an organotypical sandwich culture

Toxicology Letters

Volumn 188, Issue 3, 2009, Pages 173-179

  Langsch, Angelika ^a   Giri, Shibashish ^b   Acikgöz, Ali ^b   Jasmund, Inka ^a   Frericks, Bernd ^a   Bader, Augustinus ^b  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Albumin; Biotransformation; CYP activity; Metabolism; Metabolites; Primary human hepatocyte; Primary porcine hepatocytes; Primary rat hepatocyte; Testosterone

Indexed keywords

7 ETHOXYCOUMARIN DEETHYLASE; ALBUMIN; ETHOXYRESORUFIN DEETHYLASE; LACTATE DEHYDROGENASE; TESTOSTERONE;

ADULT; AGED; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOTRANSFORMATION; CELL ISOLATION; CELL METABOLISM; CELL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FEMALE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; LIVER CELL; LIVER FUNCTION; MALE; METABOLITE; NONHUMAN; PRIORITY JOURNAL; RAT; SPECIES DIFFERENCE; SWINE;

ADULT; AGED; ALBUMINS; ANIMALS; BIOTRANSFORMATION; CELLS, CULTURED; CYTOCHROME P-450 CYP1A1; CYTOLOGICAL TECHNIQUES; FEMALE; HEPATOCYTES; HUMANS; L-LACTATE DEHYDROGENASE; LIVER; LIVER FUNCTION TESTS; MALE; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; RATS; RATS, WISTAR; SPECIES SPECIFICITY; SWINE; TESTOSTERONE; TOXICITY TESTS;

RATTUS; SUS;

EID: 67349285377     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2009.03.022     Document Type: Article

References (50)

1. Acikgöz A., Karim N., Giri S., Schmidt-Heck W., and Bader A. Two compartment model of diazepam biotransformation in an organotypical culture of primary human hepatocytes. Toxicol. Appl. Pharmacol. 234 (2009) 179-191
2. Agius L., Wright P.D., and Alberti K.G.M.M. Carnitine acyltransferases and acyl-CoA hydrolases in human and rat liver. Clin. Sci. 73 (1987) 3-10
3. Bader A., Rinkes I.H., Closs E.I., Ryan C.M., Toner M., Cunningham J.M., Tompkins R.G., and Yarmush M.L. A stable long-term hepatocyte culture system for studies of physiologic processes: cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotechnol. Prog. 8 (1992) 219-225
4. Bader A., Zech K., Crome O., Christians U., Ringe B., Pichlmayer R., and Sewing K.F. Use of organotypical cultures of primary hepatocytes to analyse drug biotransformation in man and animals. Xenobiotica 24 (1994) 623-633
5. Bader A., DeBartolo L., and Haverich A. High level benzodiazepine and ammonia clearance by flat membrane bioreactors with porcine liver cells. J. Biotechnol. 25 (2000) 95-105
6. Bars R.G., Mitchell A.M., Wolf C.R., and Elcombe C.R. Induction of cytochrome P450 in cultured rat hepatocytes. The heterogenous localization of specific isoenzmymes using immunocytochemistry. Biochem. J. 262 (1989) 151-158
7. Berry M.N., and Friend D.S. High-yield preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43 (1969) 506-520
8. Bhunchet E., and Wake K. The portal lobule in rat liver fibrosis: a re-evaluation of the liver unit. Hepatology 27 (1998) 481-487
9. Brandon E.F.A., Raap C.D., Meijerman I., Beijnen J.H., and Schellens J.H.M. An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons. Toxicol. Appl. Pharmacol. 189 (2003) 233-246
10. Chiu S.H. The use of in vitro metabolism studies in the understanding of new drugs. J. Pharmacol. Toxicol. Methods 29 (1993) 77-83
11. Cross D.M., and Bayliss M.K. A commentary on the use of hepatocytes in drug metabolism studies during drug discovery and development. Drug Metab. Rev. 32 (2000) 219-240
12. Daoust R. The cell population of liver tissue and the cytological reference base. Ann. Inst. Biol. Sci. Publ. 4 (1958) 3-10
13. Danpure C.J. Biochemical data lactate dehydrogenase and cell injury. Cell Biochem. Funct. 2 (1984) 144-148
14. Donato M.T., Castell J.V., and Gómez-Lechón M.J. Cytochrome P450 activities in pure and co-cultured rat hepatocytes. Effects of model inducers. In Vitro Cell Dev. Biol. 30 (1994) 825-832
15. Donato M.T., Castell N.V., and Gomez-Lechon M.J. Effect of model inducers on cytochrome P450 activities of human hepatocytes in primary culture. Drug Metab. Dispos. 23 (1995) 553-558
16. Dunn J.C., Yarmush M.L., Koebe H.G., and Tompkins R.G. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J. 3 (1989) 174-177
17. Elsdale T., and Bard J. Collagen substrate for studies on cell behaviour. J. Cell Biol. 54 (1972) 626-637
18. FDA (Food and Drug Administration), Center for Drug Evaluation and Research. August, 2005. Guidance for Industry, Impurities in Drug Product. Revision I, http://www.fda.gov/Cder/Guidance/6423dftrev1.html.
19. Froehlich A.K., Girreser U., and Clement B. Metabolism of N hydroxyguanidines (N hydroxydebrisoquine) in human and porcine hepatocytes: reduction and formation of glucuronides. Drug Metab. Dispos. 33 (2005) 1532-1537
20. Gates G.A., Henley K.S., Pollard H.M., Schmidt E., and Schmidt F.W. The cell population of human liver. J. Lab. Clin. Med. 57 (1961) 182-184
21. Guillouzo A., Morel F., Fardel O., and Meunier B. Use of human hepatocyte cultures for drug metabolism studies. Toxicology 82 (1993) 209-219
22. Hanson R.W. Is there a single best species for studies of metabolic regulation?. Trends Biochem. Sci. 5 (1980) 1-2
23. Hirose, Y., Nagahori, H., Yamada, T., Deguchi, Y., Tomigahara, Y., Nishioka, K., Uwagawa, S., Kawamura, S., Isobe, N., Lake, B.G., Okuno, Y. 2009. Comparison of the effects of the synthetic pyrethroid metofluthrin and phenobarbital on CYP2B form induction and replicative DNA synthesis in cultured rat and human hepatocytes. 258, 64-69.
24. Högemann D., Baumann A., Rocker D., Bader A., and Galanski M. In vitro model of the human liver parenchyma to study hepatotoxic side effects by Dy-EOB-DTPA. Invest. Radiol. 35 (2000) 373-379
25. Hoogenboom L.A., Pastoor F.J., Clous W.E., Hesse S.E., and Kuiper H.A. The use of porcine hepatocytes for biotransformation studies of veterinary drugs. Xenobiotica 19 (1989) 1207-1219
26. Iwatsubo T., Hirota N., Ooie T., Suzuki H., Shimada N., Chiba K., Ishizaki T., Green C.E., Tyson C.A., and Sugiyama Y. Prediction of in vivo drug metabolism in the human liver from in vitro metabolism data. Pharmacol. Ther. 73 (1997) 147-171
27. Jasmund I., Schwientek S., Acikgöz A., Langsch A., Machens H.G., and Bader A. The influence of medium composition and matrix on long-term cultivation of primary porcine and human hepatocytes. Biomol. Eng. 24 (2007) 59-69
28. Jeffrey P., Warne P., and Williams R. Addressing the innovation gap. Drug News Perspect. 20 (2007) 265-269
29. Jemnitz K., Veres Z., Monostory K., Kóbori L., and Vereczkey L. Interspecies differences in acetaminophen sensitivity of human, rat, and mouse primary hepatocytes. Toxicol. In Vitro 22 (2008) 961-967
30. Kern A., Bader A., Pichlmayer R., and Sewing K.F. Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem. Pharmacol. 54 (1997) 761-772
31. Kocarek T.A., Schuetz E.G., Strom S.C., Fisher R.A., and Guzelian P.S. Comparative analysis of cytochrome P3A induction in primary cultures of rat rabbit and human hepatocytes. Drug Metab. Dispos. 23 (1994) 415-421
32. Kogure K., Ishizaki M., Nemoto M., Kuwano H., and Makuuchi M. A comparative study of the anatomy of rat and human livers. J. Hepatobiliary Pancreat. Surg. 6 (1999) 171-175
33. Kotokorpi P., Ellis E., Parini P., Nilsson L.M., Strom S., Steffensen K.R., Gustafsson J.A., and Mode A. Physiological differences between human and rat primary hepatocytes in response to liver X receptor activation by 3-[3-[N-(2-chloro-3-trifluoromethylbenzyl)-(2,2diphenylethyl) amino] propyloxy]phenylacetic acid hydrochloride (GW3965). Mol. Pharmacol. 72 (2007) 947-955
34. Langsch A., and Bader A. Long term stability of phase I and phase II enzymes of porcine liver cells in flat membrane bioreactors. Biotechnol. Bioeng. 76 (2001) 115-125
35. Li A.P. Accurate prediction of human drug toxicity: a major challenge in drug development. Chem. Biol. Interact. 150 (2004) 3-7
36. Monostory K., Kõhalmy R., Prough L., Kóbori L., and Vereczkey. The effect of synthetic glucocorticoid, dexamethasone on CYP1A1 inducibility in adult rat and human hepatocytes. FEBS Lett. 579 (2005) 229-235
37. Monshouwer M., van't Klooster G.A.E., Nijmeijer S.M., Witkamp R.F., and van Miert A.S.J.P.A.M. Characterization of cytochrome P450 isoenzymes in primary cultures of pig hepatocytes. Toxicol. In Vitro 12 (1998) 715-723
38. Olsen A.K., Hansen K.T., and Friis C. Pig hepatocytes as an in vitro model to study the regulation of human CYP3A4: prediction of drug-drug interactions with 17α-ethynylestradiol. Chem. Biol. Int. 107 (1997) 93-108
39. Padgham C.R.W., Wang X.J., and Paine A.J. The loss of cytochromes P450 (CYPs) in rat liver cell culture is triggered during hepatocyte isolation and again during the first 4 hours of culture. In: Lechner M.C. (Ed). Cytochrome P450 8th International Conference (1994), John Libbey Eurotext, Paris 539-542
40. Panies A.J. The maintenance of cytochrome P 450 in rat hepatocytes culture: Some application of liver cell culture to study of drug metabolism, toxicity and the induction of the P450 system. Chem. Biol. Interact. 74 (1990) 4141-4147
41. Rohr H.P., Luthy J., Gudat F., Oberholtzer M., Gysin C., Stadler G., and Bianchi L. A new discovery in the study of human liver biopsy specimens. In: Popper H., and Schaffner F. (Eds). Progress of Liver Diseases vol. 5 (1976), Grune and Stratton, New York 24-34
42. Schmelzer E., Acikgoez A., Fruehauf N.R., Crome O., Klempnauer J., Christians U., and Bader A. Biotransformation of cyclosporin in matrix overlaid primary rat, porcine, and human hepatocytes. Xenobiotica 36 (2006) 693-708
43. Seglen P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 13 (1976) 39-83
44. Sheil A.G. Xenogeneic bioartificial liver support: where are we now?. Transplant Proc. 34 (2002) 2493-2495
45. Smith D.A. Species differences in metabolism and pharmacokinetics: are we close to an understanding?. Drug Metab. Rec. 23 (1991) 355-373
46. Strom S.C., Pisarov L.A., Dorko K., Thompson M.T., Schuetz J.D., and Schuetz E.G. Use of human hepatocytes to study P450 gene induction. Methods Enzymol. 272 (1996) 388-401
47. Tenconi, L.T., Beretta, E., 1970. Urinary and biliary metabolites of rifampicin in different animal species. In: The Problems of Species Differences and Statistics in Toxicology, Proc. Europ. Soc. For study of drug Toxicity, Vol. II; Excerpta Med. Int. Congr. Ser. No. 198, Excerpta Medica, Amsterdam, pp. 80-85.
48. Vickers A.E., Fischer V., Connors S., Fisher R.L., Baldeck J.P., Maurer G., and Brendel K. Cyclosporin A metabolism in human liver, kidney, and intestine slices. Comparison to rat and dog slices and human cell lines. Drug Metab. Dispos. 20 (1992) 802-809
49. Wilkening S., Stahl F., and Bader A. Comparison of primary human hepatocytes and hepatoma cell line Hepg2 with regard to their biotransformation properties. Drug Metab. Dispos. 31 (2003) 1035-1042
50. Zuber R., Anzenbacherova E., and Anzenbacher P. Cytochromes P450 and experimental models of drug metabolism. J. Cell Mol. Med. 6 (2002) 189-198