Stability of freeze-dried vaginal Lactobacillus strains in the presence of different lyoprotectors

Canadian Journal of Microbiology

Volumn 55, Issue 5, 2009, Pages 544-552

  Tomás, María Silvina Juárez ^a   Bru, Elena ^a   Martos, Gladys ^a   Nader Macías, María Elena ^a  

^a CENTRO DE REFERENCIA PARA LACTOBACILOS CERELA CONICET   (Argentina)

Author keywords

Lyoprotectors; Probiotics; Storage; Vaginal lactobacilli

Indexed keywords

ANTIMICROBIAL SUBSTANCE; CUBIC POLYNOMIALS; FUNCTIONAL ACTIVITIES; INDUSTRIAL USE; LACTIC ACID BACTERIA; LONG-TERM STORAGE; LYOPROTECTORS; PROBIOTIC; PROBIOTIC CULTURES; PROBIOTICS; SKIM MILKS; STORAGE; SUSPENSION MEDIUM; VAGINAL LACTOBACILLI;

INDUSTRIAL APPLICATIONS; LACTIC ACID; ORGANIC ACIDS;

SUGAR (SUCROSE);

LACTOSE; PROBIOTIC AGENT; SUCROSE; WATER;

BACTERIUM; FREEZE DRYING; MODELING; PRESERVATION; PROBIOTICS; STORAGE; SUGAR; SURVIVAL;

ARTICLE; BACTERIAL STRAIN; BACTERIAL SURVIVAL; BACTERIAL VIABILITY; BACTERIUM CULTURE; ENZYME SYNTHESIS; FREEZE DRYING; LACTOBACILLUS; MILK; NONHUMAN; PRIORITY JOURNAL; STORAGE;

BACTERIAL ADHESION; BACTERIOCINS; COLONY COUNT, MICROBIAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; FEMALE; HUMANS; HYDROGEN PEROXIDE; LACTIC ACID; LACTOBACILLUS; LACTOSE; MICROBIAL VIABILITY; MILK PROTEINS; SUCROSE; VAGINA;

BACTERIA (MICROORGANISMS); LACTOBACILLUS;

EID: 66849121878     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W08-159     Document Type: Article

References (41)

1. Abadias, M., Benabarre, A., Teixidó, N., Usall, J., and Viñas, I. 2001. Effect of freeze drying and protectants on viability of the biocontrol yeast Candida sake. Int. J. Food Microbiol. 65(3): 173-182. doi:10.1016/S0168-1605(00)00513-4. PMID: 11393686.
2. Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X., and Gibbs, P. 2002. Survival of freeze-dried Lactobacillus plantarum and Lactobacillus rhamnosus during storage in the presence of protectants. Biotechnol. Lett. 24(19): 1587-1591. doi:10.1023/A:1020301614728.
3. Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X., and Gibbs, P. 2004. Effects of various sugars added to growth and drying media upon thermotolerance and survival throughout storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Biotechnol. Prog. 20(1): 248-254. doi:10.1021/bp034165y. PMID:14763849.
4. Castro, H.P., Teixeira, P.M., and Kirby, R. 1997. Evidence of membrane damage in Lactobacillus bulgaricus following freeze drying. J. Appl. Microbiol. 82(1): 87-94. doi:10.1111/j.1365-2672.1997.tb03301.x.
5. Falagas, M.E., Rafailidis, P.I., and Makris, G.C. 2008. Bacterial interference for the prevention and treatment of infections. Int. J. Antimicrob. Agents. 31(6): 518-522. doi:10.1016/j.ijantimicag. 2008.01.024. PMID:18359612.
6. Fonseca, F., Beal, C., and Corrieu, G.J. 2000. Method of quantifying the loss of acidification activity of lactic acid starters during freezing and frozen storage. J. Dairy Res. 67(1): 83-90. doi:10.1017/S002202999900401X. PMID:10717846. (Pubitemid 30104849)
7. Font de Valdez, G., Savoy de Giori, G., De Ruiz Holgado, A.P., and Oliver, G. 1983. Comparative study of the efficiency of some additives in protecting lactic acid bacteria against freeze-drying. Cryobiology, 20(5): 560-566. doi:10.1016/0011-2240(83)90044-5. PMID:6194937.
8. Gagné, J., Roy, D., and Gauthier, S.F. 1993. Production of frozen and freeze-dried concentrates of Bifidobacterium infantis by membrane filtration. Milchwissenschaft, 48(9): 501-505. (Pubitemid 23798801)
9. Huang, L., Lu, Z., Yuan, Y., Lü, F., and Bie, X. 2006. Optimization of a protective medium for enhancing the viability of freeze-dried Lactobacillus delbrueckii subsp. bulgaricus based on response surface methodology. J. Ind. Microbiol. Biotechnol. 33(1): 55-61. doi:10.1007/s10295-005-0041-8. PMID:16244855.
10. Johnson, J.A.C., and Etzel, M.R. 1995. Properties of Lactobacillus helveticus CNRZ-32 attenuated by spray-drying, freeze-drying, or freezing. J. Dairy Sci. 78(4): 761-768.
11. Juárez Tomás, M.S., and Nader-Macías, M.E. 2007. Effect of a medium simulating vaginal fluid on the growth and expression of beneficial characteristics of potentially probiotic lactobacilli. In Communicating current research and educational topics and trends in applied microbiology. Microbiology Series No. 1. Edited by A. Méndez-Vilas. Formatex, Badajoz, Spain. pp. 732-739.
12. Juárez Tomás, M.S., Bru de Labanda, E., de Ruiz Holgado, A.P., and Nader-Macías, M.E. 2002a. Estimation of vaginal probiotic lactobacilli growth parameters with the application of the Gompertz model. Can. J. Microbiol. 48(1): 82-92. doi:10.1139/w01-135. PMID:11888167.
13. Juárez Tomás, M.S., Bru, E., Wiese, B., De Ruiz Holgado, A.P., and Nader-Macías, M.E. 2002b. Influence of pH, temperature and culture media on the growth and bacteriocin production of vaginal Lactobacillus salivarius CRL 1328. J. Appl. Microbiol. 93(4): 714-724. doi:10.1046/j.1365- 2672.2002.01753.x. PMID: 12234356.
14. Juárez Tomás, M.S., Bru, E., and Nader-Macías, M.E. 2003a. Comparison of the growth and hydrogen peroxide production by vaginal probiotic lactobacilli under different culture conditions. Am. J. Obstet. Gynecol. 188(1): 35-44. doi:10.1067/mob.2003.123. PMID:12548193.
15. Juárez Tomás, M.S., Ocaña, V., Wiese, B., and Nader-Macías, M.E. 2003b. Growth and lactic acid production by vaginal Lactobacillus acidophilus CRL 1259. Inhibition of uropathogenic Escherichia coli. J. Med. Microbiol. 52(Pt. 12): 1117-1124. doi:10.1099/jmm.0.05155-0. PMID:14614071.
16. Juárez Tomás, M.S., Wiese, B., and Nader-Macías, M.E. 2005. Effects of culture conditions on the growth and auto-aggregation ability of vaginal Lactobacillus johnsonii CRL 1294. J. Appl. Microbiol. 99(6): 1383-1391. doi:10.1111/j.1365-2672.2005.02726.x. PMID:16313411.
17. Lepargneur, J.P., and Rousseau, V. 2002. Protective role of the Doderlein flora. J. Gynecol. Obstet. Biol. Reprod. (Paris). 31(5): 485-494. PMID:12379833.
18. Leslie, S.B., Israeli, E., Lighthart, B., Crowe, J.H., and Crowe, L.M. 1995. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying. Appl. Environ. Microbiol. 61(10): 3592-3597.
19. Lloyd, G.T. 1975. The production of concentrated starters by batch culture: II. Studies on the optimum storage temperature. Aust. J. Dairy Technol. 30: 107.
20. Maggi, L., Mastromarino, P., Macchia, S., Brigidi, P., Pirovano, F., Mateuzzi, D., and Conte, U. 2000. Technological and biological evaluation of tablets containing different strains of lactobacilli for vaginal administration. Eur. J. Pharm. Biopharm. 50(3): 389-395. doi:10.1016/S0939-6411(00)00121-1. PMID:11072196.
21. Martos, G.I., Minahk, C.J., Font de Valdez, G., and Morero, R. 2007. Effects of protective agents on membrane fluidity of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Lett. Appl. Microbiol. 45(3): 282-288. doi:10.1111/j.1472-765X. 2007.02188.x. PMID:17718840.
22. Miyamoto-Shinohara, Y., Sukenobe, J., Imaizumi, T., and Nakahara, T. 2006. Survival curves for microbial species stored by freeze-drying. Cryobiology, 52(1): 27-32. doi:10.1016/j.cryobiol.2005.09.002. PMID:16271358. (Pubitemid 43202191)
23. Miyamoto-Shinohara, Y., Sukenobe, J., Imaizumi, T., and Nakahara, T. 2008. Survival of freeze-dried bacteria. J. Gen. Appl. Microbiol. 54(1): 9-24. doi:10.2323/jgam.54.9. PMID:18323678.
24. Ocaña, V.S., and Nader-Macías, M.E. 2002. Vaginal lactobacilli: self and co-aggregating ability. Br. J. Biomed. Sci. 59(4): 183-190. PMID:12572950.
25. Ocaña, V.S., Bru, E., De Ruiz Holgado, A.P., and Nader-Macías, M.E. 1999a. Surface characteristics of lactobacilli isolated from human vagina. J. Gen. Appl. Microbiol. 45(5): 203-212. doi:10.2323/jgam.45.203. PMID:12501362.
26. Ocaña, V.S., De Ruiz Holgado, A.P., and Nader-Macías, M.E. 1999b. Characterization of a bacteriocin-like substance produced by a vaginal Lactobacillus salivarius strain. Appl. Environ. Microbiol. 65(12): 5631-5635. PMID:10584033.
27. Ocaña, V.S., De Ruiz Holgado, A.P., and Nader-Macías, M.E. 1999c. Selection of vaginal H2O2-generating Lactobacillus for probiotic use. Curr. Microbiol. 38(5): 279-284. doi:10.1007/PL00006802. PMID:10355116.
28. Palmfeldt, J., and Hahn-Hägerdal, B. 2000. Influence of culture pH on survival of Lactobacillus reuteri subjected to freeze-drying. Int. J. Food Microbiol. 55(1-3): 235-238. doi:10.1016/S0168-1605(00)00176-8. PMID:10791749.
29. Raibaud, P., Caulet, M., Galpín, J.V., and Mocquot, G. 1961. Studies on the bacterial flora of the alimentary tract of pigs, II Streptococci: selective enumeration of the dominant groups. J. Appl. Bacteriol. 24: 285-291.
30. Reid, G., Charbonneau, D., Erb, J., Kochanowski, B., Beuerman, D., Poehner, R., and Bruce, A.W. 2003. Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. FEMS Immunol. Med. Microbiol. 35(2): 131-134. doi:10.1016/S0928-8244(02)00465-0. PMID:12628548.
31. Sanders, M.E., Guarner, F., Mills, D., Pot, B., Rafter, J., Rastall, B., et al. 2005. Selected topics in probiotics and prebiotics: meeting report for the 2004 International Scientific Association for Probiotics and Prebiotics. Curr. Issues Intest. Microbiol. 6(2): 55-68. PMID:16107038.
32. Santivarangkna, C., Kulozik, U., and Foerst, P. 2007. Alternative drying processes for the industrial preservation of lactic acid starter cultures. Biotechnol. Prog. 23(2): 302-315. doi:10.1021/bp060268f. PMID:17305363.
33. Schoug, A., Fischer, J., Heipieper, H.J., Schnürer, J., and Hakansson, S. 2008. Impact of fermentation pH and temperature on freeze-drying survival and membrane lipid composition of Lactobacillus coryniformis Si3. J. Ind. Microbiol. Biotechnol. 35(3): 175-181. doi:10.1007/s10295-007-0281-x. PMID:18057973.
34. Selmer-Olsen, E., Birkeland, S.E., and Sørhaug, T. 1999. Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydration. J. Appl. Microbiol. 87(3): 429-437. doi:10.1046/j.1365-2672.1999. 00839.x. PMID:10540246.
35. Silva, J., Carvalho, A.S., Teixeira, P., and Gibbs, P.A. 2002. Bacteriocin production by spray-dried lactic acid bacteria. Lett. Appl. Microbiol. 34(2): 77-81. doi:10.1046/j.1472-765x.2002. 01055.x. PMID:11849498.
36. Tsvetkov, T., and Brankova, R. 1983. Viability of micrococci and lactobacilli upon freezing and freeze-drying in the presence of different cryoprotectants. Cryobiology, 20(3): 318-323. doi:10.1016/0011-2240(83)90020-2. PMID:6884073.
37. Van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S.D., and Maguin, E. 2002. Stress responses in lactic acid bacteria. Antonie van Leeuwenhoek, 82(1-4): 187-216. doi:10.1023/A:1020631532202. PMID:12369188.
38. Yao, A.A., Coulibaly, I., Lognay, G., Fauconnier, M.L., and Thonart, P. 2008. Impact of polyunsaturated fatty acid degradation on survival and acidification activity of freeze-dried Weissella paramesenteroides LC11 during storage. Appl. Microbiol. Biotechnol. 79(6): 1045-1052. doi:10.1007/s00253-008- 1497-z. PMID:18461316.
39. Zárate, G., Juárez Tomás, M.S., and Nader-Macías, M.E. 2005. Effect of some pharmaceutical excipients on the survival of probiotic vaginal lactobacilli. Can. J. Microbiol. 51(6): 483-489. doi:10.1139/w05-031. PMID:16121226.
40. Zayed, G., and Roos, Y.H. 2004. Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage. Process Biochem. 39(9): 1081-1086. doi:10.1016/S0032-9592(03)00222-X. (Pubitemid 38527179)
41. Zhao, G., and Zhang, G. 2005. Effect of protective agents, freezing temperature, rehydration media on viability of malolactic bacteria subjected to freeze-drying. J. Appl. Microbiol. 99(2): 333-338. doi:10.1111/j.1365-2672.2005. 02587.x. PMID:16033464.