Fluorescence lifetime imaging study of a single cell: Stress-induced environmental change and electric field effects onfluorescence

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7190, Issue , 2009, Pages

  Ohta, Nobuhiro ^a   Nakabayashi, Takakazu ^a   Nagao, Issei ^a   Kinjo, Masataka ^a   Aoki, Yumiko ^a,b   Tanaka, Minoru ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

Author keywords

Cell stress; EGFP tudor fusion protein; Electric field effect; Enhanced green fluorescent protein (EGFP); Fluorescence Lifetime Imaging (FLIM)

Indexed keywords

APPLIED ELECTRIC FIELD; CELL STRESS; CELLULAR MICROENVIRONMENT; EGFP-TUDOR FUSION PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP); ENVIRONMENTAL CHANGE; FIELD-INDUCED CHANGES; FLUORESCENCE DECAY PROFILE; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE LIFETIME IMAGING (FLIM); FLUORESCENCE LIFETIMES; FUSION PROTEINS; HELA CELL; IN-VIVO; INDIVIDUAL CELLS; LOCAL ELECTRIC FIELD; MICROENVIRONMENT; NONINVASIVE DETERMINATION; NONRADIATIVE PROCESS; PROTEIN MATRIX; SINGLE CELLS; STRESS-INDUCED;

CHROMOPHORES; ELECTRIC FIELD MEASUREMENT; FLUORESCENCE; MEDICAL IMAGING; NANOPARTICLES; PROBES; PROTEINS;

ELECTRIC FIELD EFFECTS;

EID: 66749127455     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.808484     Document Type: Conference Paper

References (41)

1. Shimomura, O., Johnson, F. H. and Saigusa, Y., "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea," J. Cell. Comp. Physiol. 59, 223-239 (1962).
2. Tsien, R. Y., "The green fluorescent protein," Annu. Rev. Biochem. 67, 509-544 (1998). (Pubitemid 28411137)
3. Zimmer, M., "Green fluorescent protein (GFP): applications, structure, and related photophysical behavior," Chem. Rev. 102, 759-781 (2002). (Pubitemid 35377613)
4. Lichtman, J. W. and Conchello, J.-A., "Fluorescence microscopy," Nat. Methods 2, 910-919 (2005). (Pubitemid 43108727)
5. Presley, J. F., Cole, N. B., Schroer, T. A., Hirschberg, K., Zaal, K. J. M. and Lippincott-Schwartz, J., "ER-to-Goldi transport visualized in living cells," Nature 389, 81-85 (1997). (Pubitemid 27387695)
6. Heim, R., Prasher, D. C. and Tsien, R. Y., "Wavelength mutations and posttranslational autoxidation of green fluorescent protain," Proc. Natl. Acad. Sci. U.S.A. 91, 12501-12504 (1994).
7. Heim, R., Cubitt, A. B. and Tsien, R. Y., "Improved green fluorescence," Nature 373, 663-664 (1995).
8. Cody, C. W., Prasher, D. C., Westler, W. M., Prendergast, F. G. and Ward, W. W., "Chemical structure of the hexapeptide chromophore of the Aequorea Green-Fluorescent Protein," Biochemistry 32, 1212-1218 (1993). (Pubitemid 23070642)
9. Ormö, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y. and Remington, S. J., "Crystal structure of the Aequorea victoria green fluorescent protein," Science 273, 1392-1395 (1996). (Pubitemid 26296528)
10. Yang, F., Moss, L. G. and Phillips, Jr. G. N., "The molecular structure of green fluorescent protein," Nat. Biotechnol. 14, 1246-1251 (1996). (Pubitemid 26332784)
11. Heim, R. and Tsien, R. Y., "Engineering green fluoescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer," Curr. Biol. 6, 178-182 (1996). (Pubitemid 2616642)
12. Yang, T.-T., Sinai, P., Green, G., Kitts, P. A., Chen, Y.-T., Lybarger, L., Chervenak, R., Patterson, G. H., Piston, D. W. and Kain, S. R., "Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein," J. Biol. Chem. 273, 8212-8216 (1998). (Pubitemid 28168877)
13. Lakowicz, J. R. and Berndt, K. W., "Lifetime-selective fluorescence imaging using an rf phase-sensitive camera," Rev. Sci. Instrum. 62, 1727-1734 (1991).
14. Hanson, K. M., Behne, M. J., Barry, N. P., Mauro, T. M., Gratton, E. and Clegg, R. M., "Two-photon fluorescence lifetime imaging of the skin stratum corneum pH gradient," Biophys. J. 83, 1682 (2002). (Pubitemid 34977738)
15. Becker, W., Bergmann, A., Hink, M. A., König, K., Benndorf, K. and Biskup, C., "Fluorescence lifetime imaging by time-correlated single-photon counting," Microsc. Res. Tech. 63, 58-66 (2004). (Pubitemid 37541971)
16. Wallrabe, H. and Periasamy, A., "Imaging protein molecules using FRET and FLIM microscopy," Curr. Opin. Biotechnol. 16, 19-27 (2005). (Pubitemid 40249755)
17. Suhling, K., Siegel, J., Phillips, D., French, P. M. W., Lévêque-Fort, S., Webb, S. E. D. and Davis, D. M., "Imaging the environment of green fluorescent protein," Biophys. J. 83, 3589-3595 (2002). (Pubitemid 36041976)
18. Treanor, B., Lanigan, P. M. P., Suhling, K., Schreiber, T., Munro, I., Neil, M. A. A., Phillips, D., Davis, D. M. and French, P. M. W., "Imaging fluorescence lifetime heterogeneity applied to GFP-tagged MHC protein at an immunological synapse," J. Microsc. 217, 36-43 (2005). (Pubitemid 40188358)
19. Harpur, A. G., Wouters, F. S. and Bastiaens, P. I. H., "Imaging FRET between spectrally similar GFP molecules in single cells," Nat. Biotechnol. 19, 167-169 (2001). (Pubitemid 32144447)
20. Krafft, C., Knetschke, T., Funk, R. H. W. and Salzer, R., "Studies on stress-induced changes at the subcellular level by Raman microspectroscopic mapping," Anal. Chem. 78, 4424-4429 (2006).
21. Huang, Y.-S., Karashima, T., Yamamoto, M. and Hamaguchi, H., "Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time-and space-resolved Raman spectroscopy," Biochemistry 44, 10009-10019 (2005). (Pubitemid 41076796)
22. Naito, Y., Toh-e, A. and Hamaguchi, H., "In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell," J. Raman Spectrosc. 36, 837-839 (2005). (Pubitemid 41345507)
23. Bardsley, A., McDonald, K. and Boswell, R. E., "Distribution of tudor protein in the Drosophila embryo suggests separation of functions based on site of localization," Development 119, 207-219 (1993). (Pubitemid 23279140)
24. Thomson, T. and Lasko, P., "Tudor and its domains: germ cell formation from a Tudor perspective," Cell Res. 15, 281-291 (2005). (Pubitemid 41642884)
25. Nagao, I., Aoki, Y., Tanaka, M. and Kinjo, M., "Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching," FEBS J. 275, 341-349 (2008).
26. Ohta, N., "Electric field effects on photochemical dynamics in solid films," Bull. Chem. Soc. Jpn. 75, 1637-1655 (2002). (Pubitemid 34959948)
27. Callis, P. R. and Burgess, B. K., "Tryptophan fluorescence shifts in proteins from hybrid simulations: an electrostatic approach," J. Phys. Chem. B 101, 9429-9432 (1997). (Pubitemid 127581253)
28. Park, E. S., Andrews, S. S., Hu, R. B. and Boxer, S. G., "Vibrational Stark spectroscopy in proteins: a probe and calibration for electrostatic fields," J. Phys. Chem. B 103, 9813-9817 (1999). (Pubitemid 129614020)
29. Kriegl, J. M., Nienhaus, K., Deng, P., Fuchs, J. and Nienhaus, G. U., "Ligand dynamics in a protein internal cavity," Proc. Natl. Acad. Sci. USA 100, 7069-7074 (2003). (Pubitemid 36706394)
30. Ohta, N., Koizumi, M., Umeuchi, S., Nishimura, Y. and Yamazaki, I., "External electric field effects on fluorescence in an electron donor and acceptor system: ethyl carbazole and dimethyl terephthalate in PMMA polymer films," J. Phys. Chem. 100, 16466-16471 (1996). (Pubitemid 126787984)
31. Ohta, N., Umeuchi, S., Nishimura, Y. and Yamazaki, I., "Electric-field-induced quenching of exciplex fluorescence and photocurrent generation in a mixture of ethyl carbazole and dimethyl terephthalate doped in a PMMA polymer film," J. Phys. Chem. B 102, 3784-3790 (1998). (Pubitemid 128584925)
32. Tsushima, M., Ushizaka, T. and Ohta, N., "Time-resolved measurement system of electrofluorescence spectra," Rev. Sci. Instrum. 75, 479-485 (2004).
33. Nakabayashi, T., Wahadoszamen, Md. and Ohta, N., "External electric field effects on state energy and photoexcitation dynamics of diphenylpolyenes," J. Am. Chem. Soc. 127, 7041-7052 (2005). (Pubitemid 40676732)
34. Gerritsen, H. C., Asselbergs, M. A. H., Agronskaia, A. V. and van Sark, W. G. J. H. M., "Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution," J. Microsc. 206, 218-224 (2002). (Pubitemid 34639419)
35. Nakabayashi, T., Iimori, T., Kinjo, M. and Ohta, N., "Construction of a fluorescence lifetime imaging system and its application to biological systems and polymer materials," J. Spec. Soc. Jpn. 55, 31-39 (2006) (in Japanese).
36. Ito, Y., Suzuki, M. and Husimi, Y., "A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation," Biochem. Biophys. Res. Commun. 264, 556-560 (1999). (Pubitemid 29516124)
37. Ito, Y., Kawama, T., Urabe, I. and Yomo, T., "Evolution of an arbitrary sequence in solubility," J. Mol. Evol. 58, 196-202 (2004). (Pubitemid 38183971)
38. Pepperkok, R., Squire, A., Geley, S. and Bastiaens, P. I. H., "Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy," Curr. Biol. 9, 269-272 (1999). (Pubitemid 29139311)
39. Hess, S. T., Sheets, E. D., Wagenknecht-Wiesner, A. and Heikal, A. A., "Quantitative analysis of the fluorescence properties of intrinsically fluorescent proteins in living cells," Biophys. J. 85, 2566-2580 (2003). (Pubitemid 37210802)
40. Heikal, A. A., Hess, S. T. and Webb W. W., "Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP): acid-base specificity," Chem. Phys. 274, 37-55 (2001). (Pubitemid 33136035)
41. Suhling, K., Davis, D. M. and Phillips, D., "The influence of solvent viscosity on the fluorescence decay and timeresolved anisotropy of green fluorescent protein," J. Fluoresc. 12, 91-95 (2002).