Mechanochemical delivery and dynamic tracking of fluorescent quantum dots in the cytoplasm and nucleus of living Cells

Nano Letters

Volumn 9, Issue 5, 2009, Pages 2193-2198

  Yum, Kyungsuk ^a   Na, Sungsoo ^a   Xiang, Yang ^b   Wang, Ning ^a   Yu, Min Feng ^a,b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL PROCESS; BIOPHYSICAL PROPERTIES; DYNAMIC TRACKING; FLUORESCENT QUANTUM DOTS; IN-CELL; LIVING CELL; MECHANOCHEMICAL; MECHANOCHEMICAL METHOD; MOLECULAR RESOLUTION; MONO-DISPERSED; NANO SCALE; TEMPORAL PRECISION;

CELL MEMBRANES; CYTOLOGY; DYNAMICS; FLUORESCENCE; MOLECULAR DYNAMICS;

SEMICONDUCTOR QUANTUM DOTS;

FLUORESCENT DYE; QUANTUM DOT;

ARTICLE; CELL NUCLEUS; CYTOPLASM; HELA CELL; HUMAN; METABOLISM; METHODOLOGY; NANOTECHNOLOGY;

CELL NUCLEUS; CYTOPLASM; FLUORESCENT DYES; HELA CELLS; HUMANS; NANOTECHNOLOGY; QUANTUM DOTS;

EID: 66449129795     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl901047u     Document Type: Article

References (43)

1. Weiss, S. Fluorescence spectroscopy of single biomolecules. Science 1999, 283, 1676-1683.
2. Xie, X. S.; Yu, J.; Yang, W. Y. Living cells as test tubes. Science 2006, 312, 228-230.
3. Evanko, D. Watching single molecules in cells. Nat Mthods 2008, 5,25.
4. Moerner, W. E. New directions in single-molecule imaging and analysis. Proc. Natl Acad. Sci. U.S.A. 2007, 104, 12596-12602.
5. Neuman, K. C.; Nagy, A. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy. Nat. Methods 2008, 5, 491-505.
6. Michalet, X.; Pinaud, F. F.; Bentolila, L. A.; Tsay, J. M.; Doose, S.; Li, J. J.; Sundaresan, G.; Wu, A. M.; Gambhir, S. S.; Weiss, S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 2005, 307, 538-544.
7. Alivisatos, A. P.; Gu, W.; Larabell, C. Quantum dots as cellular probes. Annu .Rev Biomed.Enig. 2005, 7, 55-76.
8. Resch-Genger, U.; Grabolle, M.; Cavaliere-Jaricot, S.; Nitschke, R.; Nann, T. Quantum dots versus organic dyes as fluorescent labels. Nat. Methods 2008, 5, 763-775.
9. Mannix, R. J.; Kumar, S.; Cassiola, F.; Montoya-Zavala, M.; Feinstein, E.; Prentiss, M.; Ingber, D. E. Nanomagnetic actuation of receptor- mediated signal transduction. Nat. Nanotechnol. 2008, 3, 36-40.
10. Dahan, M.; Levi, S.; Luccardini, C.; Rostaing, P.; Riveau, B.; Triller, A. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science 2003, 302, 442-445.
11. Howarth, M.; Takao, K.; Hayashi, Y.; Ting, A. Y. Targeting quantum dots to surface proteins in living cells with biotin ligase. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 7583-7588.
12. Bannai, H.; Levi, S.; Schweizer, C.; Dahan, M.; Triller, A. Imaging the lateral diffusion of membrane molecules with quantum dots. Nat. Protoc. 2007, 1, 2628-2634.
13. Howarth, M.; Liu, W.; Puthenveetil, S.; Zheng, Y.; Marshall, L. F.; Schmidt, M. M.; Wittrup, K. D.; Bawendi, M. G.; Ting, A. Y. Monovalent, reduced-size quantum dots for imaging receptors on living cells. Nat. Methods 2008, 5, 397-399.
14. Roullier, V.; Clarke, S.; You, C.; Pinaud, F.; Gouzer, G. R.; Schaible, D.; Marchi-Artzner, V. R.; Piehler, J.; Dahan, M. High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ Tris-nitrilotriacetic acid quantum dot conjugates. Nano Lett 2009, 9, 1228-1234.
15. Courty, S.; Luccardini, C.; Bellaiche, Y.; Cappello, G.; Dahan, M. Tracking individual kinesin motors in living cells using single quantum-dot imaging. Nano Lett. 2006, 6, 1491-1495.
16. Cambi, A.; Lidke, D. S.; Arndt-Jovin, D. J.; Figdor, C. G.; Jovin, T. M. Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells. Nano Lett. 2007, 7, 970-977.
17. Cui, B.; Wu, C.; Chen, L.; Ramirez, A.; Bearer, E. L.; Li, W.-P.; Mobley, W. C.; Chu, S. One at a time, live tracking of NGF axonal transport using quantum dots. Proc. Natl Acad. Sci. U.S.A. 2007, 104, 13666-13671.
18. Zhang, Q.; Cao, Y. Q.; Tsien, R. W. Quantum dots provide an optical signal specific to full collapse fusion of synaptic vesicles. Proc.Natl Acad. Sci. U.S.A. 2007, 104, 17843-17848.
19. Zhang, Q.; Li, Y.; Tsien, R. W. The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles. Science 2009, 323, 1448-1453.
20. Derfus, A. M.; Chan, W. C. W.; Bhatia, S. N. Intracellular delivery of quantum dots for live cell labeling and organelle tracking. Adv. Mater. 2004, 16, 961-966.
21. Ruan, G.; Agrawal, A.; Marcus, A. I.; Nie, S. Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding. J. Am. Chem. Soc. 2007, 129, 14759-14766.
22. Delehanty, J.; Mattoussi, H.; Medintz, I. Delivering quantum dots into cells: strategies, progress and remaining issues. Anal. Bioanal. Chem. 2009, 393, 1091-1105.
23. Panorchan, P.; Lee, J. S. H.; Daniels, B.; Kole, T. P.; Tseng, Y.; Wirtz, D. Probing cellular mechanical responses to stimuli using ballistic intracellular nanorheology. Methods Cell Biol. 2007, 83, 115-140.
24. Chen, X.; Kis, A.; Zettl, A.; Bertozzi, C. R. A cell nanoinjector based on carbon nanotubes. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 8218-8222.
25. Stephens, D. J.; Pepperkok, R. The many ways to cross the plasma membrane. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 4295-4298.
26. Dubertret, B.; Skourides, P.; Norris, D. J.; Noireaux, V.; Brivanlou, A. H.; Libchaber, A. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 2002, 298, 1795-1962.
27. Yum, K.; Cho, H.; Hu, J.; Yu, M.-F. Needle nanoprobes for electrochemical studies in picoliter microenvironments. ACS Nano 2007, 1, 440-448.
28. Arrigo, A.-P. Gene expression and the thiol redox state. Free Radical Biol. Med. 1999, 27, 936-944.
29. Schafer, F. Q.; Buettner, G. R. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radical Biol. Med. 2001, 30, 1191-1212.
30. Saito, G.; Swanson, J. A.; Lee, K. D. Drug delivery strategy utilizing conjugation via reversible disulfide linkages: role and site of cellular reducing activities. Adv. Drug Delivery Rev. 2003, 55, 199-215.
31. Love, J. C.; Estroff, L. A.; Kriebel, J. K.; Nuzzo, R. G.; Whitesides, G. M. Self-assembled monolayers of thiolates on metals as a form of nanotechnology. Chem. Rev. 2005, 105, 1103-1170.
32. Sbalzarini, I. F.; Koumoutsakos, P. Feature point tracking and trajectory analysis for video imaging in cell biology. J. Struct. Biol. 2005, 151, 182-195.
33. Luby-Phelps, K. Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area. Int. Rev. Cytol. 2000, 192, 189-221.
34. Grunwald, D.; Hoekstra, A.; Dange, T.; Buschmann, V.; Kubitscheck, U. Direct observation of single protein molecules in aqueous solution. ChemPhysChem 2006, 7, 812-815.
35. Weihs, D.; Mason, T. G.; Teitell, M. A. Bio-microrheology: a frontier in microrheology. Biophys. J. 2006, 91, 4296-4305.
36. Vargas, D. Y.; Raj, A.; Marras, S. A. E.; Kramer, F. R.; Tyagi, S. Mechanism of messenger RNA transport in the nucleus. Proc. Natl Acad. Sci. U.S.A. 2005, 102, 17008-17013.
37. Tseng, Y.; Lee, J. S. H.; Kole, T. P.; Jiang, I.; Wirtz, D. Micro- organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J. Cell Sci. 2004, 117, 2159-2167.
38. Shav-Tal, Y.; Darzacq, X.; Shenoy, S. M.; Fusco, D.; Janicki, S. M.; Spector, D. L.; Singer, R. H. Dynamics of single mRNPs in nuclei of living cells. Science 2004, 304, 1797-1800.
39. Phair, R. D.; Misteli, T. High mobility of proteins in the mammalian cell nucleus. Nature (London) 2000, 404, 604-609.
40. Meaburn, K. J.; Misteli, T. Cell biology: chromosome territories. Nature (London) 2007, 445, 379-381.
41. Na, S.; Collin, O.; Chowdhury, F.; Tay, B.; Ouyang, M.; Wang, Y.; Wang, N. Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 6626-6631.
42. Tekle, C.; Deurs, B. V.; Sandvig, K.; Iversen, T.-G. Cellular trafficking of Quantum dot-ligand bioconjugates and their induction of changes in normal routing of unconjugated ligands. Nano Lett. 2008, 8, 1858-1865.
43. Clarke, S. J.; Hollmann, C. A.; Zhang, Z.; Suffern, D.; Bradforth, S. E.; Dimitrijevic, N. M.; Minarik, W. G.; Nadeau, J. L. Photophysics of dopamine-modified quantum dots and effects on biological systems. Nat. Mater. 2006, 5, 409-417.