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When KRpTIRR was guanidinated before N-terminal derivatization, the already extremely basic peptide became even more basic with the conversion of the lysine to a homoarginine residue. This significantly reduced the abundance of the doubly charged ion, making it difficult to analyze; therefore, KRpTIRR was not guanidinated to ensure comparison of the ETcaD method with CID. This was the only lysine-containing peptide not guanidinated in this study
-
When KRpTIRR was guanidinated before N-terminal derivatization, the already extremely basic peptide became even more basic with the conversion of the lysine to a homoarginine residue. This significantly reduced the abundance of the doubly charged ion, making it difficult to analyze; therefore, KRpTIRR was not guanidinated to ensure comparison of the ETcaD method with CID. This was the only lysine-containing peptide not guanidinated in this study.
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