Effect of siRNA targeting HIF-1α combined L-ascorbate on biological behavior of hypoxic MiaPaCa2 cells

Technology in Cancer Research and Treatment

Volumn 8, Issue 3, 2009, Pages 235-240

  Chen, Chuangui ^a   Tang, Peng ^a   Yue, Jie ^a   Ren, Peng ^a   Liu, Xiangming ^a   Zhao, Xijiang ^a   Yu, Zhentao ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Hypoxia inducible factor (HIF); L ascorbate; Migration; Proliferation; Recombinant adeno associated virus (rAAV); Small interference RNA (siRNA)

Indexed keywords

ASCORBIC ACID; HYPOXIA INDUCIBLE FACTOR; SMALL INTERFERING RNA;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CELL HYPOXIA; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; NICK END LABELING; PANCREAS CANCER; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; WESTERN BLOTTING;

EID: 66049141243     PISSN: 15330346     EISSN: None     Source Type: Journal    
DOI: 10.1177/153303460900800309     Document Type: Article

References (23)

1. Yin, T., Wang, C., Liu, T., Zhao, G., Zha, Y., Yang, M. Expression of Snail in pancreatic cancer promotes metastasis and chemoresistance. J Surg Res 141, 196-203 (2007). (Pubitemid 47049639)
2. Brizel, D. M., Scully, S. P., Harrelson, J. M., Layfield, L. J., Bean, J. M., Prosnitz, L. R., Dewhirst, M. W. Tumor oxygenation predicts for the likelihood of distant metastasis in human soft tissue sarcoma. Cancer Research 56, 941-943 (1996).
3. Semenza, G. L. Targeting HIF-1 for cancer therapy. Nat Rev Cancer 3, 721-732 (2003).
4. Wang, G. L., Jiang, B. H., Rue, E. A., Semenza, G. L. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA 92, 5510-5514 (1995).
5. Masson, N., Willam, C., Maxwell, P. H., Pugh, C. W., Ratcliffe, P. J. Independent function of two destruction domains in hypoxia-inducible factor-α chains activated by prolyl hydroxylation. EMBO J 20, 5197-5206 (2001). (Pubitemid 32910914)
6. Semenza, G. L. HIF-1: mediator of physiological and pathophysiological responses to hypoxia. J Appl Physiol 88, 1474-1480 (2000).
7. Huang, L. E., Gu, J., Schau, M., Bunn, H. F. Regulation of hypoxia-inducible factor 1α is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway. Proc Natl Acad Sci USA 95, 7987-7992 (1998). (Pubitemid 28326053)
8. Jiang, B. H., Rue, E., Wang, G. L., Roe, R., Semenza, G. L. Dimerization, DNA binding, and transactivation properties of hypoxia-inducible factor 1. J Biol Chem 271, 17771-17778 (1996). (Pubitemid 26250751)
9. Büchler, P., Reber, H. A., Büchler, M., Shrinkante, S., Büchler, M. W., Friess, H., Semenza, G. L., Hines, O. J. Hypoxia-inducible factor 1 regulates vascular endothelial growth factor expression in human pancreatic cancer. Pancreas 26, 56-64 (2003). (Pubitemid 36020267)
10. Lu, H., Forbes, R. A., Verma, A. Hypoxia-inducible factor 1 activation by aerobic glycolysis implicates the Warburg effect in carcinogenesis. J Bio Chem 277, 23111-23115 (2002).
11. Wang, F., Li, S. S., Segersvard, R., Strömmer, L., Sundqvist, K. G., Holgersson, J., Permert, J. Hypoxia inducible factor-1 mediates effects of insulin on pancreatic cancer cells and disturbs host energy homeostasis. Am J Pathol 170, 469-477 (2007). (Pubitemid 47339384)
12. Zhong, H., De Marzo, A. M., Laughner, E., Lim, M., Hilton, D. A., Zagzag, D., Buechler, P., Isaacs, W. B., Semenza, G. L., Simons, J. W. Overexpression of hypoxia-inducible factor-1α in common human cancers and their metastases. Cancer Res 59, 5830-5835 (1999).
13. Westphal, S., Kalthoff, H. Apoptosis: targets in pancreatic cancer. Mol Cancer 2, 6 (2003).
14. Knowles, H. J., Raval, R. R., Harris, A. L., Ratcliffe, P. J. Effect of ascorbate on the activity of hypoxia-inducible factor in cancer cells. Cancer Res 63, 1764-1768 (2003).
15. Zamore, P. D. RNA interference: listening to the sound of silence. Nat Struct Biol 8, 746-750 (2001).
16. Tomar, R. S., Matta, H., and Chaudhary, P. M. Use of adeno-associated viral vector for delivery of small interfering RNA. Oncogene 22, 5712-5715 (2003) . (Pubitemid 37102709)
17. Stilwell, J. L., Samulski, R. J. Adeno-associated virus vectors for therapeutic gene transfer. Biotechniques 34, 148-150 (2003).
18. Wu, X. B., Dong, X. Y., Wu, Z. J., Cao, H., Niu, D. B., Qu, J. G., Wang, H., Hou, Y. D. A novel method for purification of recombinant adeno-associated virus vectors in large-scale. Chin Sci Bull 46, 485-489 (2001).
19. Kwon, S. J., Lee, Y. J. Effect of low glutamine/glucose on hypoxia-induced elevation of hypoxia-inducible factor-1α in human pancreatic cancer MiaPaCa-2 and human prostatic cancer DU-145 cells. Clin Cancer Res 11, 4694-4700 (2005).
20. Li, Q. F., Wang, X. R., Yang, Y. W., Lin, H. Hypoxia upregulates hypoxia inducible factor (HIF)-3α expression in lung epithelial cells: characterization and comparison with HIF-1α. Cell Res 16, 548-558 (2006). (Pubitemid 43920283)
21. Wincewicz, A., Sulkowska, M., Koda, M., Sulkowski, S. Cumulative expression of HIF-1-alpha, Bax, Bcl-xL and P53 in human colorectal cancer. Pathology 39, 334-338 (2007). (Pubitemid 46897599)
22. Diebold, I., Djordjevic, T., Hess, J., Görlach, A. Rac-1 promotes pulmonary artery smooth muscle cell proliferation by upregulation of plasminogen activator inhibitor-1: role of NFkappaB-dependent hypoxia-inducible factor-1alpha transcription. Thromb Haemost 100, 1021-1028 (2008).
23. Wei, H., Wang, C., Chen, L. Proliferating cell nuclear antigen, survivin, and CD34 expressions in pancreatic cancer and their correlation with hypoxia-inducible factor 1alpha. Pancreas 32, 159-163 (2006)