Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus β-glucosidase CelB by directed evolution

Biochemistry

Volumn 39, Issue 13, 2000, Pages 3656-3665

  Lebbink, Joyce H G ^a,b   Kaper, Thijs ^a   Bron, Peter ^a   Van Der Oost, John ^a   De Vos, Willem M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLUCOSIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STABILITY; EVOLUTION; HYDROLYSIS; LOW TEMPERATURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PYROCOCCUS FURIOSUS; THERMOSTABILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPARAGINE; BACTERIAL PROTEINS; BETA-GLUCOSIDASE; BINDING SITES; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ENZYME STABILITY; GENE LIBRARY; GLUCOSIDES; GLYCOSYLATION; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POINT MUTATION; PYROCOCCUS FURIOSUS; SERINE; TEMPERATURE; VARIATION (GENETICS);

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 0034603740     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi991483q     Document Type: Article

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