Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase

Protein Expression and Purification

Volumn 57, Issue 1, 2008, Pages 9-16

  Bailey, Lucas J ^a   Elsen, Nathaniel L ^a   Pierce, Brad S ^a   Fox, Brian G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Bacterial multicomponent monooxygenase; Diiron enzyme; Flavoprotein; Iron sulfur; Oxidoreductase

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; IRON; OXIDOREDUCTASE; OXYGENASE; TOLUENE 4 MONOOXYGENASE; TOLUENE-4-MONOOXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; COLORIMETRY; ELECTRON SPIN RESONANCE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HEAT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; MOLECULAR WEIGHT; PH; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN DENATURATION; SOLUBILITY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORIMETRY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; GENETIC VECTORS; HEAT; HYDROGEN-ION CONCENTRATION; HYDROXYLATION; IRON; KINETICS; MOLECULAR WEIGHT; OXIDOREDUCTASES; OXYGENASES; PHYLOGENY; PROMOTER REGIONS (GENETICS); PROTEIN DENATURATION; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 36248942454     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.09.007     Document Type: Article

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