Optimized expression and purification of toluene 4-monooxygenase hydroxylase

Protein Expression and Purification

Volumn 20, Issue 1, 2000, Pages 58-65

  Studts, Joey M ^a   Mitchell, Kevin H ^a   Pikus, Jeremie D ^a   McClay, Kevin ^b   Steffan, Robert J ^b   Fox, Brian G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b The Shaw Group Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 CRESOL; BUFFER; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENETIC STRAIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDATION; PH; TEMPERATURE; TOLUENE 4 MONOOXYGENASE HYDROXYLASE;

ESCHERICHIA COLI;

EID: 0033810394     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1281     Document Type: Article

References (35)

1. Toluene-4-monooxygenase, a three component enzyme system that catalyzes the oxidation of toluene to p-cresol in Pseudomonas mendocina KR1
2. Recombinant toluene 4-monooxygenase: Catalytic and Mossbauer studies of the purified diiron and Rieske components of a four-protein complex
3. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase
4. Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase
5. Catalysis by non-heine iron-containing enzymes
6. Dioxygen activation by enzymes containing binuclear non-heme iron clusters
7. Purification and characterization of toluene 2-monooxygenase from Burkeholderia cepacia G4
8. Sequence analysis of the gene cluster encoding toluene-3-monooxygenase from Pseudomonas pickettii PKO1
9. Nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from Pseudomonas sp. strain JS150
10. Gene organization and low regiospecificity in aromaticring hydroxylation of a benzene monooxygenase of Pseudomonas aeruginosa JI104
11. Genetics and biochemistry of phenol degradation by Pseudomonas sp. CF600
12. Alkene monooxygenase from Xanthrobacter strain Py2
13. Purification and characterization of the alkene monooxygenase from Norcardia corallina B-276
14. Alkene monooxygenase from Mycobacterium: A multicomponent enzyme
15. Efficient degradation of trichloroethylene by a recombinant Escherichia coli
16. Chloroform mineralization by toluene-oxidizing bacteria
17. Induction of toluene oxidation activity in Pseudomonas mendocina KR1 and Pseudomonas sp. strain ENVPC5 by chlorinated solvents and alkanes
18. Changes in the regiospecificity of aromatic hydroxylation produced by active site engineering in the diiron enzyme toluene 4-monooxygenase
19. Toluene mono-oxygenase catalyzed epoxidation of alkenes
20. Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis
21. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
22. Mini-Tn5 transposon derivatives for insertion mutagenesis, promotor probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria
23. Design and construction of expression plasmid vectors in Escherichia coli
24. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
25. Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): Process control yielding high levels of metal incorporated, soluble protein
26. Application of fed-batch fermentation to the preparation of isotopically labeled or selenomethionine-labeled proteins
27. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
28. 15N resonances of the Rieske ferredoxin component from toluene 4-monooxygenase
29. Analysis of Pseudomonas gene products using lacI(q)/Ptrp-lac plasmids and transposons that confer conditional phenotypes
30. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants
31. Control of in vivo proteolysis in the production of recombinant proteins
32. Resistance of Escherichia coli to tetracycline. Changes in permeability to tetracycline in Escherichia coli bearing transferable resistance factors
33. The β-lactamases of gram-negative bacteria and their role in resistance to β-lactam antibiotics