Adsorption of pDNA on microparticulate charged surface

Journal of Biotechnology

Volumn 141, Issue 1-2, 2009, Pages 47-57

  Paril, Christina ^a   Horner, David ^a   Ganja, Robert ^b   Jungbauer, Alois ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b BOEHRINGER INGELHEIM RCV GMBH AND CO KG   (Austria)

Author keywords

Anion exchange; Flocculation; Gene therapy; Microparticles; pDNA purification

Indexed keywords

ADSORPTION PROCESS; ADSORPTION PROPERTIES; ANION EXCHANGE; AVERAGE PARTICLE SIZES; BATCH ADSORPTIONS; BINDING CAPACITIES; CHARGED SURFACES; CHROMATOGRAPHIC PROCESS; CHROMATOGRAPHY MEDIAS; DYNAMIC BINDING CAPACITIES; E. COLI; GENE THERAPY; HIGH-CAPACITY MATERIALS; HIGH-SPEED; LYSATES; MICROPARTICLES; MODE OF OPERATIONS; MONOLITHIC SUPPORTS; PDNA PURIFICATION; PLASMID DNAS; QUATERNARY AMINES; SMALL PARTICLE SIZES; THEORETICAL YIELDS;

ADSORPTION; AMINES; CHROMATOGRAPHIC ANALYSIS; DESORPTION; ESCHERICHIA COLI; FLOCCULATION; FUNCTIONAL POLYMERS; GENETIC ENGINEERING; INDUSTRIAL MANAGEMENT; NEGATIVE IONS; NUCLEIC ACIDS; PARTICLE SIZE; PHASE SEPARATION; PURIFICATION; SODIUM CHLORIDE;

COLUMN CHROMATOGRAPHY;

PLASMID DNA; SODIUM CHLORIDE;

ADSORPTION; ARTICLE; CELL SURFACE; COLUMN CHROMATOGRAPHY; DESORPTION; DNA BINDING; DNA PURIFICATION; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MEMBRANE MICROPARTICLE; NONHUMAN; PARTICLE SIZE; PHASE SEPARATION; PRIORITY JOURNAL; ZETA POTENTIAL;

ADSORPTION; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; ESCHERICHIA COLI; FLOCCULATION; KINETICS; MICROSCOPY; MICROSCOPY, FLUORESCENCE; NANOPARTICLES; PARTICLE SIZE; PLASMIDS; POLYSTYRENES;

EID: 64249154143     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.02.009     Document Type: Article

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