Precipitation by polycation as capture step in purification of plasmid DNA from a clarified lysate

Biotechnology and Bioengineering

Volumn 87, Issue 5, 2004, Pages 675-684

  Wahlund, P O ^a   Gustavsson, P E ^a   Izumrudov, V A ^b   Larsson, P O ^a   Galaev, I Yu ^a  

^a LUND UNIVERSITY   (Sweden)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Capture; Plasmid DNA; Poly(N, N dimethyldiallylammonium) chloride; Polyelectrolyte complexes; Precipitation

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; GENES; POSITIVE IONS; PRECIPITATION (CHEMICAL); PROTEINS; RNA; STOICHIOMETRY; VACCINES;

LARGE-SCALE PRODUCTION; POLYCATION;

DNA;

PLASMID DNA; POLY(N,N' DIMETHYLDIALLYLAMMONIUM) CHLORIDE; POLYCATION; POLYELECTROLYTE; UNCLASSIFIED DRUG; ZEOLITE;

ARTICLE; BACTERIUM CULTURE; CELL LYSATE; CYTOLYSIS; DNA PURIFICATION; ENZYMATIC DEGRADATION; GEL ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; GENE THERAPY; ION EXCHANGE CHROMATOGRAPHY; PRECIPITATION; SCALE UP;

ABSORPTION; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; DNA, CIRCULAR; ELECTROPHORESIS, AGAR GEL; NUCLEIC ACIDS; PLASMIDS; POLYAMINES; POLYETHYLENES; PRECIPITATION; QUATERNARY AMMONIUM COMPOUNDS;

BACTERIA (MICROORGANISMS);

EID: 4644265849     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20180     Document Type: Article

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