Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications

Biotechnology Progress

Volumn 15, Issue 4, 1999, Pages 725-731

  Ferreira, Guilherme N M ^a   Cabral, Joaquim M S ^a   Prazeres, Duarte M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

DNA PURIFICATION; DOWNSTREAM PROCESSING; GENE THERAPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; PLASMID DNA;

CELLS; CHROMOSOMES; CLARIFICATION; FLOWCHARTING; GENETIC ENGINEERING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; SALTS; THERMAL EFFECTS;

ALKALINE LYSIS; CHAOTROPIC SALT; GENE THERAPY; PROCESS FLOW SHEET; SUPERCOILED PLASMIDS;

DNA;

BIOREACTORS; CELL FRACTIONATION; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; DNA, SUPERHELICAL; GENE THERAPY; PLASMIDS; RIBONUCLEASES; RNA;

ANIMALIA;

EID: 0033167256     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990065+     Document Type: Article

References (16)

1. Sikora, K. Scope and Limitations of Gene Therapy. In Gene Therapy; Lemoine, N. R., Cooper, D. N., Eds.; BIOS Scientific Publishers Limited: Oxford, U.K., 1996; pp 1-10.
2. USFDA. Addendum to The Points To Consider in Human Somatic Cell and Gene Therapy (draft). US FDA Center for Biologics Evaluation and Research, 1996.
3. Prazeres, D. M. F.; Ferreira, G. N. M.; Monteiro, G. A.; Cooney, C. L.; Cabral, J. M. S. Large-Scale Production of Pharmaceutical-Grade Plasmid DNA for Gene Therapy: Problems and Bottlenecks. Trends Biotechnol. 1999, 17, 169-174.
4. Marquet, M.; Horn, N. A.; Meek, J. A. Process Development for the Manufacture of Plasmid DNA Vectors for Use in Gene Therapy. BioPharm 1995, 26-37.
5. Marquet, M.; Horn, N. A.; Meek, J. A. Caracterization of Plasmid DNA Vectors for Use in Human Gene Therapy, Part 1. BioPharm 1997, 42-50.
6. Horn, N. A.; Meek, J. A.; Budahazi, G.; Marquet, M. Cancer Gene Therapy Using Plasmid DNA: Purification of DNA for Human Clinical Trials. Hum. Gene Ther. 1995, 6, 565-573.
7. Birnboim, H. C.; Doly, J. A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA. Nucleic Acids Res. 1979, 7, 1513-1523.
8. Rush, M. G.; Warner, R. C. Alkali Denaturation of Covalently Closed Circular Duplex Deoxyribonucleic Acid. J. Biol. Chem. 1970, 245, 2704-2708.
9. McClung, J. K.; Gonzales, R. A. Purification of Plasmid DNA by Fast Protein Liquid Chromatography on Superose 6 Preparative Grade. Anal. Biochem. 1989, 177, 378-382.
10. Chandra, G.; Patel, P.; Kost, T. A.; Gray, J. G. Large-Scale Purification of Plasmid DNA by Fast Protein Liquid Chromatography Using Hi-Load Q Sepharose Column. Anal. Biochem. 1992, 203, 169-172.
11. Prazeres, D. M.; Schluep, T.; Cooney, C. Preparative Purification of Supercoiled Plasmid DNA Using Anion-Exchange Chromatography. J. Chromatogr., A 1998, 806, 31-45.
12. Ferreira, G. N. M.; Cabral, J. M. S.; Prazeres, D. M. F. A Comparison of Gel Filtration Chromatographic Supports for Plasmid Purification. Biotechnol. Tech. 1997, 11, 417-420.
13. Lauwereys, M.; De Geus, P.; Meutter, J. D.; Stanssens, P.; Matthyssens, G. Cloning, Expression and Characterization of Cutinase, a Fungal Enzyme. In Lipases: Structure, Mechanism and Genetic Engineering, 1990; pp 243-251.
14. Lahijani, R.; Hulley, G.; Soriano, G.; Horn, N. A.; Marquet, M. High-Yield Production of pBR322-Derived Plasmids Intended for Human Gene Therapy by Employing a Temperature Controllable Point Mutation. Hum. Gene Ther. 1996, 7, 1971-1980.
15. Maniatis, T.; Fritsch, E. F.; Sambrook, J. Molecular Cloning: Laboratory Manual; Cold Spring Harbor: New York, 1982.
16. Chakrabarti, A.; Sitaric, S.; Ohi, S. A Procedure for Large-Scale Plasmid Isolation without Using Ultracentrifugation. Biotechnol. Appl. Biochem. 1992, 16, 211-215.