Chitosan N-betainates/DNA self-assembly nanoparticles for gene delivery: In vitro uptake and transfection efficiency

International Journal of Pharmaceutics

Volumn 371, Issue 1-2, 2009, Pages 156-162

  Gao, Yu ^a   Zhang, Zhiwen ^a   Chen, Lingli ^a   Gu, Wangwen ^a   Li, Yaping ^a  

^a SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Chitosan; Chitosan N betainates; DNA; Gene vector; Nanoparticles

Indexed keywords

BETAINE; CHITOSAN; CHITOSAN N BETAINATE DNA COMPLEX NANOPARTICLE; PLASMID DNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG FORMULATION; DRUG UPTAKE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PH; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; SUBSTITUTION REACTION;

ANIMALS; BETAINE; CELL LINE, TUMOR; CELL SURVIVAL; CERCOPITHECUS AETHIOPS; CHITOSAN; COS CELLS; DNA; GENE TRANSFER TECHNIQUES; HUMANS; HYDROGEN-ION CONCENTRATION; NANOPARTICLES; PHYSICOCHEMICAL PHENOMENA; TRANSFECTION;

EID: 62749135925     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2008.12.012     Document Type: Article

References (30)

1. Boonyo W., Junginger H.E., Waranuch N., Polnok A., and Pitaksuteepong T. Chitosan and trimethyl chitosan chloride (TMC) as adjuvants for inducing immune responses to ovalbumin in mice following nasal administration. J. Control. Release 121 (2007) 168-175
2. Di Colo G., Burgalassi S., Zambito Y., Monti D., and Chetoni P. Effects of different N-trimethyl chitosans on in vitro/in vivo ofloxacin transcorneal permeation. J. Pharm. Sci. 93 (2004) 2851-2862
3. Fischer D., Li Y., Ahlemeyer B., Krieglstein J., and Kissel T. In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. Biomaterials 24 (2003) 1121-1131
4. Florea B., Meaney C., Junginger H., and Borchard G. Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures. AAPS Pharm. Sci. 4 (2002) E12
5. Germershaus O., Mao S., Sitterberg J., Bakowsky U., and Kissel T. Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: establishment of structure-activity relationships in vitro. J. Control. Release 125 (2008) 145-154
6. Godbey W.T., Wu K.K., and Mikos A.G. Poly(ethylenimine)-mediated gene delivery affects endothelial cell function and viability. Biomaterials 22 (2001) 471-480
7. Hashimoto M., Morimoto M., Saimoto H., Shigemasa Y., and Sato T. Lactosylated chitosan for DNA delivery into hepatocytes: the effect of lactosylation on the physicochemical properties and intracellular trafficking of pDNA/chitosan complexes. Bioconjugate Chem. 17 (2006) 309-316
8. Holappa J., Hjálmarsdóttir M., Másson M., Rúnarsson O., Asplund T., Soininen P., Nevalainen T., and Järvinen T. Antimicrobial activity of chitosan N-betainates. Carbohydr. Polym. 65 (2006) 114-118
9. Holappa J., Nevalainen T., Savolainen J., Soininen P., Elomaa M., Safin R., Suvanto S., Pakkanen T., Másson M., Loftsson T., and Järvinen T. Synthesis and characterization of chitosan N-betainates having various degrees of substitution. Macromolecules 37 (2004) 2784-2789
10. Jiang L., Qian F., He X., Wang F., Ren D., He Y., Li K., Sun S., and Yin C. Novel chitosan derivative nanoparticles enhance the immunogenicity of a DNA vaccine encoding hepatitis B virus core antigen in mice. J. Gene Med. 9 (2007) 253-264
11. Kean T., Roth S., and Thanou M. Trimethylated chitosans as non-viral gene delivery vectors: cytotoxicity and transfection efficiency. J. Control. Release 103 (2005) 643-653
12. Kim J.H., Kim Y.S., Park K., Kang E., Lee S., Nam H.Y., Kim K., Park J.H., Chi D.Y., Park R.W., Kim I.S., Choi K., and Chan Kwon I. Self-assembled glycol chitosan nanoparticles for the sustained and prolonged delivery of antiangiogenic small peptide drugs in cancer therapy. Biomaterials 29 (2008) 1920-1930
13. Kircheis R., Wightman L., and Wagner E. Design and gene delivery activity of modified polyethylenimines. Adv. Drug Deliv. Rev. 53 (2001) 341-358
14. Lavertu M., Méthot S., Tran-Khanh N., and Buschmann M.D. High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials 27 (2006) 4815-4824
15. Liu Y., Wenning L., Lynch M., and Reineke T.M. New poly(d-glucaramidoamine)s induce DNA nanoparticle formation and efficient gene delivery into mammalian cells. J. Am. Chem. Soc. (Commun.) 126 (2004) 7422-7423
16. Mansouri S., Lavigne P., Corsi K., Benderdour M., Beaumont E., and Fernandes J.C. Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. Eur. J. Pharm. Biopharm. 57 (2004) 1-8
17. Mao S., Shuai X., Unger F., Wittmar M., Xie X., and Kissel T. Synthesis, characterization and cytotoxicity of poly(ethylene glycol)-graft-trimethyl chitosan block copolymers. Biomaterials 26 (2005) 6343-6356
18. Mao Z., Ma L., Jiang Y., Yan M., Gao C., and Shen J. N,N,N-Trimethylchitosan chloride as a gene vector: synthesis and application. Macromol. Biosci. 7 (2007) 855-863
19. Mehier-Humbert S., Bettinger T., Yan F., and Guy R.H. Ultrasound-mediated gene delivery: kinetics of plasmid internalization and gene expression. J. Control. Release 104 (2005) 203-211
20. Müller R.H., Jacobs C., and Kayser O. Nanosuspensions as particulate drug formulations in therapy: rationale for development and what we can expect for the future. Adv. Drug Deliv. Rev. 47 (2001) 3-19
21. Sakkinen M., Linna A., Ojala S., Jürjenson H., Veski P., and Marvola M. In vivo evaluation of matrix granules containing microcrystalline chitosan as a gel-forming excipient. Int. J. Pharm. 250 (2003) 227-237
22. Sieval A.B., Thanou M., Kotze A.F., Verhoef J.C., Brussee J., and Junginger H.E. Preparation and NMR characterization of highly substituted N-trimethyl chitosan chloride. Carbohydr. Polym. 36 (1998) 157-165
23. Thanou M., Florea B.I., Geldof M., Junginger H.E., and Borchard G. Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines. Biomaterials 23 (2002) 153-159
24. Thanou M.M., Kotzé A.F., Scharringhausen T., Lueßen H.L., de Boer A.G., Verhoef J.C., and Junginger H.E. Effect of degree of quaternization of N-trimethyl chitosan chloride for enhanced transport of hydrophilic compounds across intestinal Caco-2 cell monolayers. J. Control. Release 64 (2000) 15-25
25. Vassel B., and Skelly W.G. N-Chlorobetainyl chloride. Org. Synth. 4 (1963) 154
26. Venkatesh S., and Smith T.J. Chitosan-membrane interactions and their probable role in chitosan-mediated transfection. Biotechnol. Appl. Biochem. 27 (1998) 265-267
27. Zambito Y., Zaino C., Uccello-Barretta G., Balzano F., and Di Colo G. Improved synthesis of quaternary ammonium-chitosan conjugates (N(+)-Ch) for enhanced intestinal drug permeation. Eur. J. Pharm. Sci. 33 (2008) 343-350
28. Zaric V., Weltin D., Erbacher P., Remy J.S., Behr J.P., and Stephan D. Effective polyethylenimine-mediated gene transfer into human endothelial cells. J. Gene Med. 6 (2004) 176-184
29. Zelikin A.N., Putnam D., Shastri P., Langer R., and Izumrudov V.A. Aliphatic ionenes as gene delivery agents: elucidation of structure-function relationship through modification of charge density and polymer length. Bioconjugate Chem. 13 (2002) 548-553
30. Zhao X., Yu S.B., Wu F.L., Mao Z.B., and Yu C.L. Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles. J. Control. Release. 112 (2006) 223-228