Effective polyethylenimine-mediated gene transfer into human endothelial cells

Journal of Gene Medicine

Volumn 6, Issue 2, 2004, Pages 176-184

  Zaric, Violeta ^a   Weltin, Denis ^a   Erbacher, Patrick ^c   Remy, Jean Serge ^b   Behr, Jean Paul ^b   Stephan, Dominique ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b CNRS   (France)

^c Bioparc   (France)

Author keywords

Gene transfer; HUVEC; Polyethylenimine

Indexed keywords

DNA; GLUCOSE; GREEN FLUORESCENT PROTEIN; INTERCALATING AGENT; LUCIFERASE; POLYETHYLENEIMINE; POLYETHYLENEIMINE DERIVATIVE; POLYMER; SODIUM CHLORIDE;

ARTICLE; CARDIOVASCULAR DISEASE; CELL SURFACE; CELL TRANSPORT; CELL VACUOLE; CONCENTRATION (PARAMETERS); CONFOCAL MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; ENDOTHELIUM CELL; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENE TARGETING; GENE TRANSFER; GENE VECTOR; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INCUBATION TIME; INTERNALIZATION; PRIORITY JOURNAL; REPORTER GENE; SERUM; UMBILICAL VEIN; METABOLISM; PLASMID;

DNA; ENDOTHELIAL CELLS; FLOW CYTOMETRY; HUMANS; MICROSCOPY, CONFOCAL; PLASMIDS; POLYETHYLENEIMINE; SERUM; TRANSFECTION; UMBILICAL VEINS;

EID: 4644286010     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.484     Document Type: Article

References (40)

1. Baumgartner I, Isner JM. Somatic gene therapy in the cardiovascular system. Annu Rev Physiol 2001; 63: 427-450.
2. Kader KN, Sweany JM, Bellamkonda RV. Cationic lipid-mediated transfection of bovine aortic endothelial cells inhibits their attachment. J Biomed Mater Res 2002; 60: 405-410.
3. Khurana R, Martin JF, Zachary I. Gene therapy for cardiovascular disease: a case for cautious optimism. Hypertension 2001; 38: 1210-1216.
4. Tanner FC, Carr DP, Nabel GJ, et al. Transfection of human endothelial cells. Cardiovasc Res 1997; 35: 522-528.
5. Liu Y, Liggitt D, Zhong W, et al. Cationic liposome-mediated intravenous gene delivery. J Biol Chem 1995; 270: 24 864-24 870.
6. Pfeifer A, Verma IM. Gene therapy: promises and problems. Annu Rev Genomics Hum Genet 2001; 2: 177-211.
7. Commander H. Biotechnology industry responds to gene therapy death. Nat Med 2000; 6: 118.
8. Niidome T, Huang L. Gene therapy progress and prospects: nonviral vectors. Gene Ther 2002; 9: 1647-1652.
9. Abdallah B, Hassan A, Benoist C, et al. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine. Hum Gene Ther 1996; 7: 1947-1954.
10. Boussif O, Lezoualc'h F, Zanta MA, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 1995; 92: 7297-7301.
11. Fischer D, Bieber T, Li Y, et al. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm Res 1999; 16: 1273-1279.
12. Bragonzi A, Boletta A, Biffi A, et al. Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs. Gene Ther 1999; 6: 1995-2004.
13. Koping-Hoggard M, Tubulekas I, Guan H, et al. Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo. Gene Ther 2001; 8: 1108-1121.
14. Boussif O, Zanta MA, Behr JP. Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold. Gene Ther 1996; 3: 1074-1080.
15. Goula D, Benoist C, Mantero S, et al. Polyethylenimine-based intravenous delivery of transgenes to mouse lung. Gene Ther 1998; 5: 1291-1295.
16. Goula D, Remy JS, Erbacher P, et al. Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system. Gene Ther 1998; 5: 712-717.
17. Zou SM, Erbacher P, Remy JS, et al. Systemic linear polyethylenimine (L-PEI)-mediated gene delivery in the mouse. J Gene Med 2000; 2: 128-134.
18. Merlin JL, Dolivet G, Dubessy C, et al. Improvement of nonviral p53 gene transfer in human carcinoma cells using glucosylated polyethylenimine derivatives. Cancer Gene Ther 2001; 8: 203-210.
19. Poulain L, Ziller C, Muller CD, et al. Ovarian carcinoma cells are effectively transfected by polyethylenimine (PEI) derivatives. Cancer Gene Ther 2000; 7: 644-652.
20. Jaffe EA, Nachman RL, Becker CG, et al. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-2756.
21. Klein-Soyer C, Hemmendinger S, Cazenave JP. Culture of human vascular endothelial cells on a positively charged polystyrene surface, primaria: comparison with fibronectin-coated tissue culture grade polystyrene. Biomaterials 1989; 10: 85-90.
22. Felgner PL, Barenholz Y, Behr JP, et al. Nomenclature for synthetic gene delivery systems. Hum Gene Ther 1997; 8: 511-512.
23. Labat-Moleur F, Steffan AM, Brisson C, et al. An electron microscopy study into the mechanism of gene transfer with lipopolyamines. Gene Ther 1996; 3: 1010-1017.
24. Mislick KA, Baldeschwieler JD. Evidence for the role of proteoglycans in cation-mediated gene transfer. Proc Natl Acad Sci U S A 1996; 93: 12 349-12 354.
25. Erbacher P, Bettinger T, Belguise-Valladier P, et al. Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI). J Gene Med 1999; 1: 210-222.
26. Ogris M, Steinlein P, Kursa M, et al. The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 1998; 5: 1425-1433.
27. Wightman L, Kircheis R, Rossler V, et al. Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo. J Gene Med 2001; 3: 362-372.
28. Kichler A, Leborgne C, Coeytaux E, et al. Polyethylenimine-mediated gene delivery: a mechanistic study. J Gene Med 2001; 3: 135-144.
29. Bettinger T, Remy JS, Erbacher P. Size reduction of galactosylated PEI/DNA complexes improves lectin-mediated gene transfer into hepatocytes. Bioconjug Chem 1999; 10: 558-561.
30. Helander IM, Alakomi HL, Latva-Kala K, et al. Polyethyleneimine is an effective permeabilizer of gram-negative bacteria. Microbiology 1997; 143: 3193-3199.
31. Helander IM, Latva-Kala K, Lounatmaa K. Permeabilizing action of polyethyleneimine on Salmonella typhimurium involves disruption of the outer membrane and interactions with lipopolysaccharide. Microbiology 1998; 144: 385-390.
32. Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine)-mediated gene delivery affects endothelial cell function and viability. Biomaterials 2001; 22: 471-480.
33. Godbey WT, Wu KK, Mikos AG. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci U S A 1999; 96: 5177-5181.
34. Gebhart CL, Kabanov AV. Evaluation of polyplexes as gene transfer agents. J Control Release 2001; 73: 401-416.
35. Uchida E, Mizuguchi H, Ishii-Watabe A, et al. Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol Pharm Bull 2002; 25: 891-897.
36. Brunner S, Sauer T, Carotta S, et al. Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus. Gene Ther 2000; 7: 401-407.
37. Brunner S, Furtbauer E, Sauer T, et al. Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation. Mol Ther 2002; 5: 80-86.
38. Pollard H, Remy JS, Loussouarn G, et al. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 1998; 273: 7507-7511.
39. Wagner E, Ogris M, Zauner W. Polylysine-based transfection systems utilizing receptor-mediated delivery. Adv Drug Deliv Rev 1998; 30: 97-113.
40. Remy-Kristensen A, Clamme JP, Vuilleumier C, et al. Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes. Biochim Biophys Acta 2001; 1514 21-32.