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2942666493
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note
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n respectively: 1, 3.0 kDa, 2.0; 2, 3.4 kDa, 1.4; 3, 3.9 kDa, 1.4; 4, 4.9 kDa, 1.6.
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26
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2942638321
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note
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α) indicate randomly coiled linear polymers.
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27
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14844304884
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2942670886
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note
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The amide nitrogens are not counted in this ratio since they will not display a positive charge within the physiological pH range (7.4-5.0).
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29
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2942673081
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note
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Polyplex sizes were determined with a Zetapals dynamic light scattering instrument (Brookhaven) at λ = 662 nm. Polyplexes were formed by combining each polymer (1-4) at an N/P ratio of 30 with 3μg of pDNA in 300 μL of DNAse free water. After 1 h, each solution was diluted with DNase free water to 0.7 mL. The results are reported as an average of 10 measurements: 256 nm (1), 441 nm (2), 140 nm (3), and 175 nm (4). TEM experimental details are given in the Supporting Information.
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30
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2942666494
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note
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2. Media was replaced after 4 and 24 h following initial transfection (see Supporting Information).
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0029160083
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