Using directed evolution to probe the substrate specificity of mandelamide hydrolase

Protein Engineering, Design and Selection

Volumn 22, Issue 2, 2009, Pages 103-110

  Wang, Pan Fen ^a   Yep, Alejandra ^a   Kenyon, George L ^a   McLeish, Michael J ^a,b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Enzyme catalysis; Homology model; Mutagenesis; Protein engineering; Selection

Indexed keywords

ACTIVE SITES; AMIDASE; AROMATIC SUBSTRATES; BINDING POCKETS; CATALYTIC TRIADS; DIRECTED EVOLUTIONS; ENZYME CATALYSIS; HOMOLOGY MODEL; HYDROLASE; ORDERS OF MAGNITUDES; PROTEIN ENGINEERING; SELECTION; SELECTION METHODS; SUBSTRATE BINDINGS; SUBSTRATE RECOGNITION; SUBSTRATE SPECIFICITIES; THREE ORDERS OF MAGNITUDES; X-RAY STRUCTURES;

BIOCHEMICAL ENGINEERING; CATALYSIS; ENZYMES; GENETIC ENGINEERING; MUTAGENESIS;

SUBSTRATES;

AMIDASE; AMIDE; AROMATIC AMIDE; GLYCINE; LACTAMIDE; MANDELAMIDE HYDROLASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CATALYSIS; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC VARIABILITY; HYDROLYSIS; MUTAGENESIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY; WILD TYPE;

AMIDES; AMIDOHYDROLASES; AMMONIA; BACTERIAL PROTEINS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE LIBRARY; MANDELIC ACIDS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN ENGINEERING; PSEUDOMONAS PUTIDA; SUBSTRATE SPECIFICITY;

EID: 61849089924     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzn073     Document Type: Article

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