Soluble MD2 increases TLR4 levels on the epithelial cell surface

Cellular Immunology

Volumn 255, Issue 1-2, 2009, Pages 8-16

  Lauer, Sabine ^a   Kunde, Yuliya A ^a   Apodaca, Theresa A ^c   Goldstein, Byron ^b   Hong Geller, Elizabeth ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b LOS ALAMOS NATIONAL LABORATORY   (United States)

^c Northern New Mexico College   (United States)

Author keywords

Endocytosis; Epithelial cells; Innate immunity; LPS signaling; Soluble MD2; Toll like receptor 4

Indexed keywords

PROTEIN MD 2; TOLL LIKE RECEPTOR 4;

ARTICLE; CELL SURFACE; CONTROLLED STUDY; DOWN REGULATION; ENDOCYTOSIS; EPITHELIUM CELL; HUMAN; HUMAN CELL; OUTCOME ASSESSMENT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN METABOLISM; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

ANIMALS; CELL LINE; CELL MEMBRANE; EPITHELIAL CELLS; HUMANS; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; TOLL-LIKE RECEPTOR 4;

EID: 59549096529     PISSN: 00088749     EISSN: 10902163     Source Type: Journal    
DOI: 10.1016/j.cellimm.2008.08.009     Document Type: Article

References (36)

1. Akira S. Mammalian toll-like receptors. Curr. Opin. Immunol. 15 (2003) 5-11
2. Aderem A., and Ulevitch R.J. Toll-like receptors in the induction of the innate immune response. Nature 406 6797 (2000) 782-787
3. Medzhitov R., Preston-Hurlburt P., and Janeway C. A human homologue of the Drosophila toll protein signals activation of adaptive immunity. Nature 388 (1997) 394-397
4. Poltorak A., He X., Smirnova I., Liu M., Van Huffel C., Du X., et al. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in TLR4 gene. Science 282 (1998) 2085-2088
5. Gioannini T., Teghanemt A., Zhang D., Coussens N., Dockstader W., Ramaswamy S., et al. Isolation of an endotoxin-MD-2 complex that produces toll-like receptor 4-dependent cell activation at picomolar concentrations. Proc. Natl. Acad. Sci. 101 (2004) 4186-4191
6. Wright S.D., Ramos R.A., Tobias P.S., Ulevitch R.J., and Mathison J.C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249 4975 (1990) 1431-1433
7. Tobias P.S., and Ulevitch R.J. Lipopolysaccharide binding protein and CD14 in LPS dependent macrophage activation. Immunobiology 187 3-5 (1993) 227-232
8. Visintin A., Mazzoni A., Spitzer J., and Segal D. Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to toll-like receptor 4. Proc. Natl. Acad. Sci. USA 98 (2001) 12156-12161
9. Visintin A., Halmen K.A., Latz E., Monks B.G., and Golenbock D.T. Pharmacological inhibition of endotoxin responses is achieved by targeting the TLR4 coreceptor, MD-2. J. Immunol. 175 10 (2005) 6465-6472
10. Re F., and Strominger J. Monomeric recombinant MD-2 binds toll-like receptor 4 tightly and confers lipopolysaccharide responsiveness. J. Biol. Chem. 277 (2002) 23427-23432
11. Shimazu R., Akashi S., Ogata H., Nagai Y., Fukudome K., Miyake K., et al. a molecule that confers lipopolysaccharide responsiveness on toll-like receptor 4. J. Exp. Med. 189 (1999) 1777-1782
12. Nagai Y., Akashi S., Nagafuku M., Ogata M., Iwakura Y., Akira S., et al. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat. Immunol. 3 7 (2002) 667-672
13. Schromm A.B., Lien E., Henneke P., Chow J.C., Yoshimura A., Heine H., et al. Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling. J. Exp. Med. 194 1 (2001) 79-88
14. Viriyakosol S., Tobias P., Kitchens R., and Kirkland T. MD-2 binds to bacterial lipopolysaccharide. J. Biol. Chem. 276 (2001) 38044-38051
15. Hyakushima N., Mitsuzawa H., Nishitani C., Sano H., Kuronuma K., Konishi M., et al. Interaction of soluble form of recombinant extracellular TLR4 domain with MD-2 enables lipopolysaccharide binding and attenuates TLR4-mediated signaling. J. Immunol. 173 11 (2004) 6949-6954
16. Arditi M., Zhou J., Dorio R., Rong G.W., Goyert S.M., and Kim K.S. Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14. Infect. Immun. 61 8 (1993) 3149-3156
17. Jia H., Kline J., Penisten A., Apicella M., Gioannini T., Weiss J., et al. Endotoxin responsiveness of human airway epithelial is limited by low expression of MD-2. Am. J. Physiol. Lung Cell. Mol. Physiol. 287 (2003) L428-L437
18. Viriyakosol S., McCray P.B., Ashbaugh M.E., Chu J., Jia H.P., Weiss J., et al. Characterization of monoclonal antibodies to human soluble MD-2 protein. Hybridoma (Larchmt) 25 6 (2006) 349-357
19. Kennedy M.N., Mullen G.E., Leifer C.A., Lee C., Mazzoni A., Dileepan K.N., et al. A complex of soluble MD-2 and lipopolysaccharide serves as an activating ligand for toll-like receptor 4. J. Biol. Chem. 279 33 (2004) 34698-34704
20. Yang H., Young D.W., Gusovsky F., and Chow J.C. Cellular events mediated by lipopolysaccharide-stimulated toll-like receptor 4. MD-2 is required for activation of mitogen-activated protein kinases and Elk-1. J. Biol. Chem. 275 27 (2000) 20861-20866
21. Pugin J., Stern-Voeffray S., Daubeuf B., Matthay M., Elson G., and Dunn-Siegrist I. Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock. Blood 104 (2004) 4071-4079
22. Lauer S.A., and Nolan J.P. Development and characterization of Ni-NTA-bearing microspheres. Cytometry 48 3 (2002) 136-145
23. Lauer S., Goldstein B., Nolan R.L., and Nolan J.P. Analysis of cholera toxin-ganglioside interactions by flow cytometry. Biochemistry 41 6 (2002) 1742-1751
24. Chow J.C., Young D.W., Golenbock D.T., Christ W.J., and Gusovsky F. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J. Biol. Chem. 274 16 (1999) 10689-10692
25. Mullen G.E., Kennedy M.N., Visintin A., Mazzoni A., Leifer C.A., Davies D.R., et al. The role of disulfide bonds in the assembly and function of MD-2. Proc. Natl. Acad. Sci. USA 100 7 (2003) 3919-3924
26. Husebye H., Halaas O., Stenmark H., Tunheim G., Sandanger O., Bogen B., et al. Endocytic pathways regulate toll-like receptor 4 signaling and link innate and adaptive immunity. EMBO J. 25 4 (2006) 683-692
27. Viriyakosol S., Tobias P., and Kirkland T. Mutational analysis of membrane and soluble forms of human MD-2. J. Biol. Chem. 281 (2006) 11955-11964
28. Abreu M.T., Arnold E.T., Thomas L.S., Gonsky R., Zhou Y., Hu B., et al. TLR4 and MD-2 expression is regulated by immune-mediated signals in human intestinal epithelial cells. J. Biol. Chem. 277 23 (2002) 20431-20437
29. Rallabhandi P., Bell J., Boukhvalova M.S., Medvedev A., Lorenz E., Arditi M., et al. Analysis of TLR4 polymorphic variants: new insights into TLR4/MD-2/CD14 stoichiometry, structure, and signaling. J. Immunol. 177 1 (2006) 322-332
30. Chuang T.H., and Ulevitch R.J. Triad3, an E3 ubiquitin-protein ligase regulating toll-like receptors. Nat. Immunol. 5 5 (2004) 495-502
31. Nagai Y., Shimazu R., Ogata H., Akashi S., Sudo K., Yamasaki H., et al. Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 99 5 (2002) 1699-1705
32. Ohnishi T., Muroi M., and Tanamoto K. MD-2 is necessary for the toll-like receptor 4 protein to undergo glycosylation essential for its translocation to the cell surface. Clin. Diagn. Lab. Immunol. 10 3 (2003) 405-410
33. Visintin A., Halmen K.A., Khan N., Monks B.G., Golenbock D.T., and E L. MD-2 expression is not required for cell surface targeting of toll-like receptor 4 (TLR4). J. Leukoc. Biol. 80 6 (2006) 1584-1592
34. Randow F., and Seed B. Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability. Nat. Cell Biol. 3 10 (2001) 891-896
35. Konno K., Wakabayashi Y., Akashi-Takamura S., Ishii T., Kobayashi M., Takahashi K., et al. A molecule that is associated with toll-like receptor 4 and regulates its cell surface expression. Biochem. Biophys. Res. Commun. 339 4 (2006) 1076-1082
36. Wakabayashi Y., Kobayashi M., Akashi-Takamura S., Tanimura N., Konno K., Takahashi K., et al. A protein associated with toll-like receptor 4 (PRAT4A) regulates cell surface expression of TLR4. J. Immunol. 177 3 (2006) 1772-1779