The biological and ethical basis of the use of human embryonic stem cells for in vitro test systems or cell therapy

Altex

Volumn 25, Issue 3, 2008, Pages 163-190

  Leist, Marcel ^a   Bremer, Susanne ^b   Brundin, Patrik ^c   Hescheler, Jürgen ^d   Kirkeby, Agnete ^e,f   Krause, Karl Heinz ^g   Pörzgen, Peter ^h   Pucéat, Michel ⁱ   Schmidt, Mathias ^j   Schrattenholz, André ^k   Zak, Naomi B ^l   Hentze, Hannes ^m  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b JOINT RESEARCH CENTRE   (Italy)

^c LUND UNIVERSITY   (Sweden)

^d UNIVERSITY OF COLOGNE   (Germany)

^e MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^f H LUNDBECK A S   (Denmark)

^g UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^h HAWAII PACIFIC UNIVERSITY   (United States)

ⁱ I Stem   (France)

^j Nycomed GmbH   (Germany)

^k PROTEOSYS AG   (Germany)

^l CellCure Neurosciences Ltd   (Israel)

^m S Bio Ltd   (Singapore)

more..

Author keywords

Human embryonic stem cells; In vitro toxicology; iPs cells; Stem cell ethics

Indexed keywords

ANIMAL TESTING ALTERNATIVE; BIOETHICS; CELL THERAPY; CONFLICT; CULTURAL FACTOR; EMBRYONIC STEM CELL; EUROPEAN UNION; HUMAN; HUMAN DIGNITY; MARKETING; PATENT; PERSONHOOD; POLICY; REVIEW; ANIMAL; ANIMAL WELFARE; ARTICLE; CELL LINE; DRUG EFFECT; EMBRYO RESEARCH; ETHICS; METHODOLOGY; STEM CELL TRANSPLANTATION;

ANIMAL TESTING ALTERNATIVES; ANIMAL WELFARE; ANIMALS; CELL LINE; EMBRYO RESEARCH; EMBRYONIC STEM CELLS; HUMANS; STEM CELL TRANSPLANTATION;

EID: 58949087263     PISSN: 09467785     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (87)

1. Baharvand, H., Ashtiani, S. K., Valojerdi, M. R. et al. (2004). Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst. Differentiation 72 5, 224-229. (Pubitemid 41702131)
2. Baker, M. (2005). Stem cell therapy or snake oil? Nat. Biotechnol. 23(12), 1467-1469.
3. Baker, M. (2007). Funding crunch forces stem cell company to abandon therapies. Nat. Biotechnol. 25(9), 951-952. (Pubitemid 47517619)
4. Baker, M. (2008). Embryonic stem-cell trial put on hold. Nature Reports Stem Cells online, http://www.nature.com/news/2008/080519/full/news.2008.842. html
5. Balls, M., Coecke, S., Bowe, G. et al. (2006). The Importance of Good Cell Culture Practice (GCCP). ALTEX 23 Suppl., 270-273.
6. Björklund, A., Dunnett, S. B., Brundin, P. et al. (2003). Neural transplantation for the treatment of Parkinson's disease. Lancet Neurol. 2(7), 437-445. (Pubitemid 36793939)
7. Bongso, A., Fong, C. Y., Ng, S. C., Ratnam, S. (1994). Human embryonic behavior in a sequential human oviduct-endometrial coculture system. Fertil. Steril. 61(5), 976-978.
8. Breitbach, M., Bostani, T., Roell, W. et al. (2007). Potential risks of bone marrow cell transplantation into infarcted hearts. Blood 110(4), 1362-1369. (Pubitemid 47281438)
9. Bremer, S., Hartung, T. (2004). The use of embryonic stem cells for regulatory developmental toxicity testing in vitro - the current status of test development. Curr. Pharm. Des. 10(22), 2733-2747. (Pubitemid 39137172)
10. Broxmeyer, H. E. (2005). Biology of cord blood cells and future prospects for enhanced clinical benefit. Cytotherapy 7(3), 209-218. (Pubitemid 41051363)
11. Buesen, R., Visan, A., Genschow, E. et al. (2004). Trends in improving the embryonic stem cell test (EST): an overview. ALTEX 21(1), 15-22. (Pubitemid 38979464)
12. Chung, Y., Klimanskaya, I., Becker, S. et al. (2008). Human embryonic stem cell lines generated without embryo destruction. Cell Stem Cell 2(2), 113-117
13. Chung, Y., Klimanskaya, I., Becker, S. et al. (2006). Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Nature 439(7073), 216-219.
14. Crook, J., Peura, T. T., Kravets, L. et al. (2008). The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1(5), 490-494.
15. Cyranoski, D. (2008). Stem cells: 5 things to know before jumping on the iPS bandwagon. Nature 452(7186), 406-408.
16. French, A. J., Adams, C. A., Anderson, L. S. et al. (2008). Development of human cloned blastocysts following somatic cell nuclear transfer with adult fibroblasts. Stem Cells 26(2), 485-493.
17. Gstraunthaler, G. (2006). Standardisation in Cell and Tissue Culture - The Need for Specific GLP Guidelines in the Cell Culture Laboratory (Good Cell Culture Practice - GCCP). ALTEX 23 Suppl., 274-277.
18. Guan, K., Schmidt, M. M., Ding, Q. et al. (1999). Embryonic Stem Cells in vitro - Prospects for Cell and Developmental Biology, Embryotoxicology and Cell Therapy. ALTEX 16(3), 135-141.
19. Guenin, L. M. (2005). A proposed stem cell research policy. Stem Cells 23, 1023-1027. (Pubitemid 41377716)
20. Hall, V. J., Li, J. Y., Brundin, P. (2007). Restorative cell therapy for Parkinson's disease: a quest for the perfect cell. Semin. Cell Dev. Biol. 18(6), 859-869. (Pubitemid 350138454)
21. Hanna, J., Markoulaki, S., Schorderet, P. et al. (2008). Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency. Cell 133(2), 250-264.
22. Hanna, J., Wernig, M., Markoulaki, S. et al. (2007). Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 318(5858), 1920-1923.
23. Hansson, M. G., Helgesson, G., Wessman, R., Jaenisch, R. (2007). Commentary: isolated stem cells - patentable as cultural artifacts? Stem Cells 25(6), 1507-1510.
24. Hartung, T. (2001). Three Rs potential in the development and quality control of pharmaceuticals. ALTEX 18 Suppl. 1, 3-13.
25. Hartung, T. (2007a). Food for thought... on validation. ALTEX 24, 67-73.
26. Hartung, T. (2007b). Food for thought... on cell culture. ALTEX 24, 143-147.
27. Hartung, T. (2008). Food for thought... on animal tests. ALTEX 25, 3-10.
28. Hartung, T., Leist, M. (2008). Food for thought... on the evolution of toxicology and phasing out of animal testing. ALTEX 25, 91-97.
29. Hentze, H., Graichen, R., Colman, A. (2007). Cell therapy and the safety of embryonic stem cell-derived grafts. Trends Biotechnol. 25(1), 24-32. (Pubitemid 44959090)
30. Johnson, M. H. (2008). Human ES cells and a blastocyst from one embryo: exciting science but conflicting ethics? Cell Stem Cell 2(2), 103-104.
31. Klemm, M., Schrattenholz, A. (2004). Neurotoxicity of active compounds - establishment of hESC-lines and proteomics technologies for human embryo- and neurotoxicity screening and biomarker identification. ALTEX 21 Suppl. 3, 41-48. (Pubitemid 38979456)
32. Klimanskaya, I., Chung, Y., Becker, S. et al. (2006) Human embryonic stem cell lines derived from single blastomeres. Nature 444(7118), 481-485.
33. Kolossov, E., Bostani, T., Roell, W. et al. (2006). Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium. J. Exp. Med. 203(10), 2315-2327. (Pubitemid 44498366)
34. Kuhn, T. S. (1970). The Structure of Scientific Revolutions, 2nd. ed., p. 206. Chicago: Univ. of Chicago Pr.
35. Leist, M. (2006). What can a chair on alternatives to animal experimentation effectuate? ALTEX 23, 211-213.
36. Leist, M., Hartung,T., Nicotera, P. (2008a). The dawning of a new age of toxicology. ALTEX 25, 103-114.
37. Leist, M., Kadereit, S., Schildknecht, S. (2008b). Food for thought... on the real success of 3R approaches. ALTEX 25, 17-24.
38. Lerou, P. H., Yabuuchi, A., Huo, H. et al. (2008a). Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos. Nat. Protoc. 3(5), 923-933.
39. Lerou, P. H., Yabuuchi, A., Huo, H. et al. (2008b). Human embryonic stem cell derivation from poor-quality embryos. Nat. Biotechnol. 26(2), 212-214.
40. Li, J. Y., Englund, E., Holton, J. L. et al. (2008). Lewy bodies in grafted neurons in subjects with Parkinson's disease suggest host-to-graft disease propagation. Nat. Med. 14(5), 501-503.
41. Lowry, W. E., Richter, L., Yachechko, R. et al. (2008). Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc. Natl. Acad. Sci. USA 105(8), 2883-2888.
42. Luttun, A., Verfaillie, C. M. (2006). A perspective on stem cells as a tool for in vitro testing. ALTEX 23 Suppl., 388-392.
43. Maio, G. (2006). Cloning in the media and popular culture. An analysis of German documentaries reveals beliefs and prejudices that are common elsewhere. EMBO Rep. 7(3), 241-245.
44. MBBNET (2008). http://mbbnet.umn.edu/scmap.html
45. McLaren, A. (2007). A scientist's view of the ethics of human embryonic stem cell research. Cell Stem Cell 1(1), 23-26.
46. Meissner, A., Jaenisch, R. (2006). Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts. Nature 439(7073), 212-215. (Pubitemid 43083143)
47. Moore, T., Ball, M. (2004). Kaguya, the first parthenogenetic mammal - engineering triumph or lottery winner? Reproduction 128(1), 1-3. (Pubitemid 39077415)
48. Nakagawa, M., Koyanagi, M., Tanabe, K. et al. (2008). Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 26(1), 101-106.
49. Nygren, J. M., Jovinge, S., Breitbach, M. et al. (2004). Bone marrow-derived hematopoietic cells generate cardiomyocytes at a low frequency through cell fusion, but not transdifferentiation. Nat. Med. 10(5), 494-501.
50. Pellizzer, C., Bremer, S., Hartung, T. (2005). Developmental toxicity testing from animal towards embryonic stem cells. ALTEX 22(2), 47-57. (Pubitemid 40974877)
51. Pickering, S. J., Minger, S. L., Patel, M. et al. (2005). Generation of a human embryonic stem cell line encoding the cystic fibrosis mutation deltaF508, using preimplantation genetic diagnosis. Reprod. Biomed. Online 10(3), 390-397. (Pubitemid 40372676)
52. Plomer, A., Taymor, K. S., Scott, C. T. (2008). Challenges to human embryonic stem cell patents. Cell Stem Cell 2(1), 13-17.
53. Popper, Karl R. (1965). .The logic of scientific discovery, 4th ed., p. 479. London: Verlag Hutchinson.
54. Revazova E. S., Turovets N. A., Kochetkova O. D. et al. (2007). Patient-specific stem cell lines derived from human parthenogenetic blastocysts. Cloning Stem Cells 9, 432-449.
55. Revazova, E. S., Turovets, N. A., Kochetkova, O. D. et al. (2008). HLA homozygous stem cell lines derived from human parthenogenetic blastocysts. Cloning Stem Cells 10(1), 11-24.
56. Revel, A., Safran, A., Laufer, N. et al. (2004). Twin delivery following 12 years of human embryo cryopreservation: case report. Hum. Reprod. 19(2), 328-329. (Pubitemid 38220001)
57. Revel, M. (2003). Human reproductive cloning, embryo stem cells and germline gene intervention: an Israeli perspective. Med. Law 22(4), 701-732. (Pubitemid 38379726)
58. Rispin, A. S., Stitzel, K. (2006). Ensuring quality of in vitro alternative test methods. ALTEX 23 Suppl., 278-283.
59. Robertson, J. A. (2001). Human embryonic stem cell research: ethical and legal issues. Nat. Rev. Genet. 2(1), 74-78. (Pubitemid 33674772)
60. Sangiorgi, E., Capecchi, M. R. (2008). Bmi1 is expressed in vivo in intestinal stem cells. Nature Genetics 40(7), 915-920.
61. SC4SM (2008). http://www.sc4sm.org/
62. Schoemans, H., Theunissen, K., Maertens, J. et al. (2006). Adult umbilical cord blood transplantation: a comprehensive review. Bone Marrow Transplant 38(2), 83-93.
63. Schrattenholz, A., Klemm, M. (2007). Neuronal cell culture from human embryonic stem cells as in vitro model for neuroprotection. ALTEX 24(1), 9-15. (Pubitemid 46563940)
64. Schuklenk, U. (2008). How not to win an ethical argument: embryo stem cell research revisited. Bioethics 22(2), ii-iii.
65. Seiler, A., Buesen, R., Hayess, K. et al. (2006). Current status of the embryonic stem cell test: the use of recent advances in the field of stem cell technology and gene expression analysis. ALTEX 23 Suppl., 393-399.
66. Seiler, A., Visan, A., Pohl, I. et al. (2002). Etablierung molekularer Endpunkte zur Weiterentwicklung des Embryonalen Stammzelltests (EST) mit embryonalen Stammzellen der Maus (Zelllinie D3) [Improving the embryonic stem cell test (EST) by establishing molecular endpoints of tissue specific development using murine embryonic stem cells (D3 cells)]. ALTEX 19 Suppl. 1, 55-63.
67. Singer, Peter (1993). Practical Ethics, 2nd. ed. Cambridge: Cambridge University Press. (Praktische Ethik, 2. Aufl., 1993, Stuttgart: Reklam.)
68. Smith, S., Neaves, W., Teitelbaum, S. (2006). Adult stem cell treatments for diseases? Science 313(5786), 439. (Pubitemid 44148429)
69. Solter, D. (2006). From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research. Nat. Rev. Genet. 7(4), 319-327.
70. Strübing, C., Wobus, A. M., Hescheler, J. (1995). Etablierung eines in vitro Modellsystems zur Differenzierung synaptisch verknüpfter Nervenzellen aus pluripotenten embryonalen Stammzellen der Maus [Establishment of an in vitro model system for the differentiation of synaptically coupled neurons from mouse embryonic stem cells]. ALTEX 12(3), 129-137.
71. Takahashi, K., Tanabe, K., Ohnuki, M. et al. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5), 861-872.
72. Takahashi, K., Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4), 663-676. (Pubitemid 44233629)
73. Tannert, C. (2006). Thou shalt not clone. An ethical argument against the reproductive cloning of humans. EMBO Rep. 7(3), 238-240.
74. Teramura, T., Takehara, T., Kishi, N. et al. (2007). A mouse and embryonic stem cell derived from a single embryo. Cloning Stem Cells 9(4), 485-494.
75. Thomas, C. (2006). Preimplantation genetic diagnosis: development and regulation. Med. Law 25(2), 365-378. (Pubitemid 44202952)
76. Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S. et al. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282(5391), 1145-1147.
77. Trounson, A., Klein, R., Murphy, R. (2008). Stem cell research in California: the game is on. Cell 132(4), 522-524.
78. UKTI (2005). http://www.chrismason.com/industry-library/assets/ UKTI%20Stem%20Cell%20Res.pdf
79. Vogel, G. (2005). Ready or not? Human ES cells head toward the clinic. Science 308(5728), 1534-1538.
80. Wellbery, C. (2000). Emergency contraception. Arch. Fam. Med. 9(7), 642-646.
81. Wernig, M., Meissner, A., Foreman, R. et al. (2007). In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448(7151), 318-324.
82. Whitlow, S., Bürgin, H., Clemann, N. (2007). The embryonic stem cell test for the early selection of pharmaceutical compounds. ALTEX 24(1), 3-7. (Pubitemid 46563939)
83. Wobus, A. M., Hescheler, J. (1992). Entwicklung eines in vitro-Herzzell-Modells für embryotoxikologische und pharmakologische Studien [Development of an in vitro cardiomyocytes cell model for embryotoxicological and pharmacological studies]. ALTEX 9(2), 29-42.
84. Xu, X. Q., Zweigerdt, R., Soo, S. Y. et al. (2008). Highly enriched cardiomyocytes from human embryonic stem cells. Cytotherapy, (in press).
85. Yu, J., Vodyanik, M. A., Smuga-Otto, K. et al. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858), 1917-1920.
86. Zollner, U., Zollner, K.-P., Steck, T. (2003). Blastozystenkultur unter den Bedingungen des Deutschen Embryonenschutzgesetzes. Gynäkologische Endokrinologie 1, 176-182.
87. Zoloth, L. (2002). Stem cell research: a target article collection: Part I - Jordan's banks, a view from the first years of human embryonic stem cell research. Am. J. Bioeth. 2(1), 3-11.