A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Wang, Xiaofeng ^a   Brandsma, Martin ^a   Tremblay, Reynald ^a   Maxwell, Denis ^a,d   Jevnikar, Anthony M ^b,c   Huner, Norm ^a,d   Ma, Shengwu ^a,b,c  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b LAWSON HEALTH RESEARCH INSTITUTE   (Canada)

^c Plantigen Inc   (Canada)

^d The University of Western Ontario   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; ANTIGENS; CHEMICAL REACTORS; CHLOROPHYLL; COSMIC RAYS; DNA; GENES; MAMMALS; NUCLEIC ACIDS; ORGANIC ACIDS; PLANTS (BOTANY); PROTEINS; SUGAR (SUCROSE);

ANTIGENICITY; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST GENOMES; EXPRESSION SYSTEMS; GLUTAMIC ACID DECARBOXYLASE; IMMUNOBLOTTING; IMMUNOREACTIVITY; LOW YIELDS; MAMMALIAN CELL CULTURES; MASS PRODUCTIONS; NOD MOUSE; PARTICLE BOMBARDMENTS; POTENTIAL SOLUTIONS; POTENTIAL VALUES; RECOMBINANT PROTEINS; SPLEEN CELLS; TOTAL SOLUBLE PROTEINS; TRANSGENIC; TYPE 1 DIABETES;

CELL CULTURE;

AUTOANTIGEN; GLUTAMATE DECARBOXYLASE; RECOMBINANT PROTEIN; ALGAL DNA; GLUTAMATE DECARBOXYLASE 2;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ANIMAL CELL; ANTIGENICITY; ARTICLE; BIOLISTIC TRANSFORMATION; BIOREACTOR; CELL PROLIFERATION; CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; CONTROLLED STUDY; DIABETES MELLITUS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION SYSTEM; GENOME; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNOREACTIVITY; MOUSE; NONHUMAN; NONOBESE DIABETIC MOUSE; PROMOTER REGION; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPLEEN CELL; TRANSGENIC ORGANISM; ANIMAL; BIOSYNTHESIS; CELL CULTURE; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; HUMAN; METABOLISM;

ALGAE; BACTERIA (MICROORGANISMS); CHLAMYDOMONAS REINHARDTII; EUKARYOTA; MAMMALIA; MUS;

ANIMALS; AUTOANTIGENS; CELLS, CULTURED; CHLAMYDOMONAS REINHARDTII; CHLOROPLASTS; DNA, ALGAL; GENE EXPRESSION; GLUTAMATE DECARBOXYLASE; HUMANS; MICE; MICE, INBRED NOD; ORGANISMS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

EID: 58249116675     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-87     Document Type: Article

References (51)

1. Goddijn OJM Pen J Plants as bioreactors Trends Biotechnol 1995, 13(9):379-387. 10.1016/S0167-7799(00)88985-4
2. Boehm R Bioproduction of therapeutic proteins in the 21st century and the role of plants and plant cells as production platforms Ann N Y Acad Sci 2007, 1102:121-134. 10.1196/annals.1408.009 17470916
3. Ma SW Tremblay R Wang XF Jevnikar AM Plant-based pharmaceuticals and its application in oral tolerance Immune Tolerance Research Development New York: Nova Science Publishers Pontell EB 2008, 79-95.
4. Ma JK Drake PM Christou P The production of recombinant pharmaceutical proteins in plants Nat Rev Genet 2003, 4(10):794-805. 10.1038/nrg1177 14526375
5. Thanavala Y Yang YF Lyons P Mason HS Arntzen C Immunogenicity of transgenic plant-derived hepatitis B surface antigen Proc Natl Acad Sci USA 1995, 92(8):3358-3361. 42165 7724566 10.1073/pnas.92.8.3358
6. Wang XG Zhang GH Liu CX Zhang YH Xiao CZ Fang RX Purified cholera toxin B subunit from transgenic tobacco plants possesses authentic antigenicity Biotechnol Bioeng 2001, 72(4):490-494. 10.1002/ 1097-0290(20010220)72:4<490::AID-BIT1011>3.0.CO;2-0 11180069
7. Jani D Singh NK Bhattacharya S Meena LS Singh Y Upadhyay SN Sharma AK Tyagi AK Studies on the immunogenic potential of plant-expressed cholera toxin B subunit Plant Cell Rep 2004, 22(7):471-477. 10.1007/ s00299-003-0711-9 14517689
8. Daniell H Production of biopharmaceuticals and vaccines in plants via the chloroplast genome Biotechnol J 2006, 1(10):1071-1079. 10.1002/ biot.200600145 17004305
9. Daniell H Lee SB Panchal T Wiebe PO Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts J Mol Biol 2001, 311(5):1001-1009. 10.1006/ jmbi.2001.4921 11531335
10. Farran I Sanchez-Serrano JJ Medina JF Prieto J Mingo-Castel AM Targeted expression of human serum albumin to potato tubers Transgenic Res 2002, 11(4):337-346. 10.1023/A:1016356510770 12212837
11. Fernandez-San Millan A Mingo-Castel A Miller M Daniell H A chloroplast transgenic approach to hyper-express and purify Human Serum Albumin, a protein highly susceptible to proteolytic degradation Plant Biotechnol J 2003, 1(2):71-79. 10.1046/j.1467-7652.2003.00008.x 17147744
12. Watson J Koya V Leppla SH Daniell H Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/ feed crop Vaccine 2004, 22(31-32):4374-4384. 10.1016/ j.vaccine.2004.01.069 15474731
13. Koya V Moayeri M Leppla SH Daniell H Plant-based vaccine: Mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge Infect Immun 2005, 73(12):8266-8274. 1307059 16299323 10.1128/IAI.73.12.8266-8274.2005
14. Tregoning JS Nixon P Kuroda H Svab Z Clare S Bowe F Fairweather N Ytterberg J van Wijk KJ Dougan G Maliga P Expression of tetanus toxin Fragment C in tobacco chloroplasts Nucleic Acids Res 2003, 31(4):1174-1179. 150239 12582236 10.1093/nar/gkg221
15. Hennig A Bonfig K Roitsch T Warzecha H Expression of the recombinant bacterial outer surface protein A in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis FEBS J 2007, 274(21):5749-5758. 10.1111/j.1742-4658.2007.06095.x 17922845
16. Arlen PA Falconer R Cherukumilli S Cole A Cole AM Oishi KK Daniell H Field production and functional evaluation of chloroplast-derived interferon-alpha2b Plant Biotechnol J 2007, 5(4):511-525. 10.1111/ j.1467-7652.2007.00258.x 17490449 2596645
17. Leelavathis S Reddy VS Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors Mol Breed 2003, 11(1):49-58. 10.1023/A:1022114427971
18. Ruhlman T Ahangari R Devine A Samsam M Daniell H Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts - oral administration protects against development of insulitis in non-obese diabetic mice Plant Biotechnol J 2007, 5(4):495-510. 10.1111/ j.1467-7652.2007.00259.x 17490448 2590789
19. Verma D Daniell H Chloroplast vector systems for biotechnology applications Plant Physiol 2007, 145(4):1129-1143. 10.1104/pp.107.106690 18056863 2151729
20. Kamarajugadda S Daniell H Chloroplast-derived anthrax and other vaccine antigens: Their immunogenic and immunoprotective properties Expert Rev Vaccines 2006, 5(6):839-849. 10.1586/14760584.5.6.839 17184221
21. Mayfield SP Manuell AL Chen S Wu J Tran M Siefker D Muto M Marin-Navarro J Chlamydomonas reinhardtii chloroplasts as protein factories Curr Opin Biotechnol 2007, 18:126-133. 10.1016/j.copbio.2007.02.001 17317144
22. Mayfield SP Franklin SE Lerner RA Expression and assembly of a fully active antibody in algae Proc Natl Acad Sci USA 2003, 100(2):438-442. 141013 12522151 10.1073/pnas.0237108100
23. Manuell AL Beligni MV Elder JH Siefker DT Tran M Weber A McDonald TL Mayfield SP Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast Plant Biotechnol J 2007, 5(3):402-412. 10.1111/ j.1467-7652.2007.00249.x 17359495
24. Sun M Qian K Su N Chang H Liu J Shen G Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast Biotechnol Lett 2003, 25(13):1087-1092. 10.1023/ A:1024140114505 12889819
25. Yang Z Li Y Chen F Li D Zhang Z Liu Y Zheng D Wang Y Shen G Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast Chinese Science Bulletin 2006, 51(14):1703-1709. 10.1007/s11434-006-2041-0
26. He DM Qian KX Shen GF Zhang ZF Li YN Su ZL Shao HB Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts Colloids Surf B Biointerfaces 2007, 55(1):26-30. 10.1016/j.colsurfb.2006.10.042 17188850
27. Franklin SE Mayfield SP Recent developments in the production of human therapeutic proteins in eukaryotic algae Expert Opin Biol Ther 2005, 5(2):225-235. 10.1517/14712598.5.2.225 15757384
28. Atkinson MA Maclaren NK The pathogenesis of insulin-dependent diabetes mellitus N Engl J Med 1994, 331(21):1428-1436. 10.1056/ NEJM199411243312107 7969282
29. Atkinson MA Eisenbarth GS Type 1 diabetes: New perspectives on disease pathogenesis and treatment Lancet 2001, 358(9277):221-229. 10.1016/ S0140-6736(01)05415-0 11476858
30. Atkinson MA Maclaren NK Scharp DW Lacy PE Riley WJ 64,000 Mr autoantibodies as predictors of insulin-dependent diabetes Lancet 1990, 335(8702):1357-1360. 10.1016/0140-6736(90)91241-2 1971659
31. Ellis TM Atkinson MA The clinical significance of an autoimmune response against glutamic acid decarboxylase Nat Med 1996, 2(2):148-153. 10.1038/ nm0296-148 8574952
32. Sabbah E Kulmala P Veijola R Vahasalo P Karjalainen J Tuomilehto-Wolf E Akerblom HK Knip M Glutamic acid decarboxylase antibodies in relation to other autoantibodies and genetic risk markers in children with newly diagnosed insulin-dependent diabetes. Childhood Diabetes in Finland Study Group J Clin Endocrinol Metab 1996, 81(7):2455-2459. 10.1210/ jc.81.7.2455 8675560
33. Tisch R Yang XD Singer SM Liblau RS Fugger L McDevitt HO Immune response to glutamic acid decarboxylase correlates with insulitis in non-obese diabetic mice Nature 1993, 366(6450):72-75. 10.1038/366072a0 8232539
34. Kaufman DL Clare-Salzler M Tian J Forsthuber T Ting GS Robinson P Atkinson MA Sercarz EE Tobin AJ Lehmann PV Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes Nature 1993, 366(6450):69-72. 10.1038/366069a0 7694152
35. Weiner HL Oral tolerance: Immune mechanisms and treatment of autoimmune diseases Immunol Today 1997, 18(7):335-343. 10.1016/ S0167-5699(97)01053-0 9238837
36. Yoon JW Sherwin RS Kwon H Jun HS Has GAD a central role in type 1 diabetes? J Autoimmun 2000, 15(3):273-278. 10.1006/jaut.2000.0442 11040067
37. Ma S Huang Y Yin Z Menassa R Brandle JE Jevnikar AM Induction of oral tolerance to prevent diabetes with transgenic plants requires glutamic acid decarboxylase (GAD) and IL-4 Proc Natl Acad Sci USA 2004, 101(15):5680-5685. 397475 15056761 10.1073/pnas.0307420101
38. Boynton JE Gillham NW Harris EH Hosler JP Johnson AM Jones AR Randolph-Anderson BL Robertson D Klein TM Shark KB Chloroplast transformation in Chlamydomonas with high velocity microprojectiles Science 1988, 240(4858):534-1538. 10.1126/science.2897716 2897716
39. Newman SM Boynton JE Gillham NW Randolph-Anderson BL Johnson AM Harris EH Transformation of chloroplast ribosomal RNA genes in Chlamydomonas: molecular and genetic characterization of integration events Genetics 1990, 126(4):875-888. 1204285 1981764
40. Goldschmidt-Clermont M Transgenic expression of aminoglycoside adenine transferase in the chloroplast: A selectable marker of site-directed transformation of chlamydomonas Nucleic Acids Res 1991, 19(15):4083-4089. 328544 1651475 10.1093/nar/19.15.4083
41. Mauch L Seissler J Haubruck H Cook NJ Abney CC Berthold H Wirbelauer C Liedvogel B Scherbaum WA Northemann W Baculovirus-mediated expression of human 65 kDa and 67 kDa glutamic acid decarboxylases in SF9 insect cells and their relevance in diagnosis of insulin-dependent diabetes mellitus J Biochem 1993, 113(6):699-704. 8370667
42. Papouchado ML Valdez SN Ghiringhelli D Poskus E Ermacora MR Expression of properly folded human glutamate decarboxylase 65 as a fusion protein in Escherichia coli Eur J Biochem 1997, 246(2):350-359. 10.1111/ j.1432-1033.1997.00350.x 9208924
43. Cambiaire JC Otis C Turmel M Lemieux C The chloroplast genome sequence of the green alga Leptosira terrestris: Multiple losses of the inverted repeat and extensive genome rearrangements within the Trebouxiophyceae BMC Genomics 2007, 8:213-225. 1931444 17610731 10.1186/1471-2164-8-213
44. Franklin S Ngo B Efuet E Mayfield SP Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast Plant J 2002, 30(6):733-744. 10.1046/j.1365-313X.2002.01319.x 12061904
45. Barnes D Franklin S Schultz J Henry R Brown E Coragliotti A Mayfield SP Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes Mol Genet Genomics 2005, 274(6):625-636. 10.1007/s00438-005-0055-y 16231149
46. Kasai S Yoshimura S Ishikura K Takaoka Y Kobayashi K Kato K Shinmyo A Effect of coding regions on chloroplast gene expression in Chlamydomonas reinhardtii J Biosci Bioeng 2003, 95(3):276-282. 16233405
47. Mayfield SP Schultz J Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast Plant J 2004, 37(3):449-458. 10.1046/j.1365-313X.2003.01965.x 14731263
48. Rowley MJ Mackay IR Chen QY Knowles WJ Zimmet PZ Antibodies to glutamic acid decarboxylase discriminate major types of diabetes mellitus Diabetes 1992, 41(4):548-551. 10.2337/diabetes.41.4.548 1607079
49. Pietropaolo M Peakman M Pietropaolo SL Zanone MM Foley TP Jr Becker DJ Trucco M Combined analysis of GAD65 and ICA512(IA-2) autoantibodies in organ and non-organ-specific autoimmune diseases confers high specificity for insulin-dependent diabetes mellitus J Autoimmun 1998, 11(1):1-10. 10.1006/jaut.1997.0170 9480718
50. Scriver C Beaudet A Sly W Valle D Type 1 Diabetes: An Autoimmune Disease The Metabolic and Molecular Bases of Inherited Disease New York: McGraw-Hill Scriver C, Beaudet A, Sly W, Valle D 1995, 859-863.
51. Quinn A Antigen-induced T1D in NOD mice J Autoimmun 2003, 20(3):207-210. 10.1016/S0896-8411(03)00028-3 12753805