Chloroplast expression of His-tagged GUS-fusions: A general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors

Molecular Breeding

Volumn 11, Issue 1, 2003, Pages 49-58

  Leelavathi, Sadhu ^a   Reddy, Vanga Siva ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

Affinity chromatography; Chloroplast expression; GUS fusion; His tag; Interferon gamma

Indexed keywords

ANTIBIOTICS; BIOCONVERSION; BIOREACTORS; ESCHERICHIA COLI; GLYCOPROTEINS; INDUSTRIAL PLANTS; PLANTS (BOTANY); RECOMBINANT PROTEINS; TOBACCO;

AFFINITY TAG; CHLOROPLAST EXPRESSION; CRITICAL FACTORS; FUSION TAG; GUS-FUSION; HIGH LEVEL EXPRESSION; HIS-TAG; INTERFERON-GAMMA; TAG-BASED; TRANSGENEIC PLANTS;

AFFINITY CHROMATOGRAPHY;

ESCHERICHIA COLI; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 0037283651     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022114427971     Document Type: Article

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