메뉴 건너뛰기




Volumn 48, Issue 1, 2009, Pages 32-43

Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae) in environmental samples

Author keywords

Karlodinium veneficum; Pfiesteria; Pseudopfiesteria shumwayae; Real time PCR; Red tide; SYTO9

Indexed keywords

CRYPTOPERIDINIOPSIS SP. CCMP1828; DINOPHYCEAE; KARLODINIUM; PFIESTERIA; PFIESTERIA PISCICIDA;

EID: 58149516918     PISSN: 00318884     EISSN: None     Source Type: Journal    
DOI: 10.2216/08-52.1     Document Type: Article
Times cited : (19)

References (53)
  • 3
    • 33645234919 scopus 로고    scopus 로고
    • On the identity of Karlodinium veneficum and description of Karlodinium arminger sp. nov. (Dinophyceae), based on light and electron microscopy, nuclear-encodede LSU rDNA, and pigment composition
    • BERGHOLTZ T., DAUGBJERG N., MOESTRUP Ø. & FERNÁNDEZ-TEJEDOR M. 2005. On the identity of Karlodinium veneficum and description of Karlodinium arminger sp. nov. (Dinophyceae), based on light and electron microscopy, nuclear-encodede LSU rDNA, and pigment composition. Journal of Phycology 42: 170-193.
    • (2005) Journal of Phycology , vol.42 , pp. 170-193
    • BERGHOLTZ, T.1    DAUGBJERG, N.2    MOESTRUP, Ø.3    FERNÁNDEZ-TEJEDOR, M.4
  • 5
    • 2642638790 scopus 로고    scopus 로고
    • Pfiesteria piscicida and other Pfiesteria-like dinoflagellates: Behavior, impacts, and environmental controls
    • BURKHOLDER J.M. & GLASGOW H.B. 1997. Pfiesteria piscicida and other Pfiesteria-like dinoflagellates: behavior, impacts, and environmental controls. Limnology and Oceanography 42: 1052-1055.
    • (1997) Limnology and Oceanography , vol.42 , pp. 1052-1055
    • BURKHOLDER, J.M.1    GLASGOW, H.B.2
  • 6
    • 0034860616 scopus 로고    scopus 로고
    • Overview and present status of the toxic Pfiesteria complex
    • BURKHOLDER J.M., GLASGOW H.B. & DEAMER-MELIA N.J. 2001. Overview and present status of the toxic Pfiesteria complex. Phycologia 40: 106-134.
    • (2001) Phycologia , vol.40 , pp. 106-134
    • BURKHOLDER, J.M.1    GLASGOW, H.B.2    DEAMER-MELIA, N.J.3
  • 7
    • 0035908647 scopus 로고    scopus 로고
    • Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular techniques
    • COYNE K.J., HUTCHINS D.A., HARE C.E. & CARY S.C. 2001. Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular techniques. Aquatic Microbial Ecology 24: 275-285.
    • (2001) Aquatic Microbial Ecology , vol.24 , pp. 275-285
    • COYNE, K.J.1    HUTCHINS, D.A.2    HARE, C.E.3    CARY, S.C.4
  • 8
    • 0012835689 scopus 로고    scopus 로고
    • Toxic activity from cultures of Karlodinium micrum (= Gyrodinium galatheanum) (Dinophyceae) - a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility
    • DEEDS J.R., TERLIZZI D.E., ADOLF J.E., STOECKER D.K. & PLACE A.R. 2002. Toxic activity from cultures of Karlodinium micrum (= Gyrodinium galatheanum) (Dinophyceae) - a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility. Harmful Algae 1: 169-189.
    • (2002) Harmful Algae , vol.1 , pp. 169-189
    • DEEDS, J.R.1    TERLIZZI, D.E.2    ADOLF, J.E.3    STOECKER, D.K.4    PLACE, A.R.5
  • 9
    • 33845341092 scopus 로고    scopus 로고
    • Histopathological effects in fish exposed to the toxins from Karlodinium micrum
    • DEEDS J.R., REIMSCHUESSEL R. & PLACE A.R. 2006. Histopathological effects in fish exposed to the toxins from Karlodinium micrum. Journal of Aquatic Animal Health 18: 136-148.
    • (2006) Journal of Aquatic Animal Health , vol.18 , pp. 136-148
    • DEEDS, J.R.1    REIMSCHUESSEL, R.2    PLACE, A.R.3
  • 10
    • 27844440297 scopus 로고    scopus 로고
    • Karlodinium australe sp. nov. (Gymnodiniales, Dinophyceae), a new potentially ichthyotoxic unarmoured dinoflagellate from lagoonal habitats of south-eastern Australia
    • DE SALAS, M.F., BOLCH C.J.S. & HALLEGRAEFF G.M. 2005. Karlodinium australe sp. nov. (Gymnodiniales, Dinophyceae), a new potentially ichthyotoxic unarmoured dinoflagellate from lagoonal habitats of south-eastern Australia. Phycologia 44: 640-650.
    • (2005) Phycologia , vol.44 , pp. 640-650
    • DE SALAS, M.F.1    BOLCH, C.J.S.2    HALLEGRAEFF, G.M.3
  • 11
    • 38949200253 scopus 로고    scopus 로고
    • Novel unarmored dinoflagellates from the toxigenic family Kareniaceae (Gymnodiniales): Five new species of Karlodinium and one new Takayama from the Australian sector of the southern ocean
    • DE SALAS, M.F., LAZA-MARTÍNEZ A. & HALLEGRAEFF G.M. 2008. Novel unarmored dinoflagellates from the toxigenic family Kareniaceae (Gymnodiniales): five new species of Karlodinium and one new Takayama from the Australian sector of the southern ocean. Journal of Phycology 44: 241-257.
    • (2008) Journal of Phycology , vol.44 , pp. 241-257
    • DE SALAS, M.F.1    LAZA-MARTÍNEZ, A.2    HALLEGRAEFF, G.M.3
  • 12
    • 33644852180 scopus 로고    scopus 로고
    • Molecular quantification of toxic Alexandrium fundyense in the Gulf of Maine using real-time PCR
    • DYHRMAN S.T., ERDNER D., DU J.L., GALAC M. & ANDERSON D.M. 2006. Molecular quantification of toxic Alexandrium fundyense in the Gulf of Maine using real-time PCR. Harmful Algae 5: 242-250.
    • (2006) Harmful Algae , vol.5 , pp. 242-250
    • DYHRMAN, S.T.1    ERDNER, D.2    DU, J.L.3    GALAC, M.4    ANDERSON, D.M.5
  • 13
    • 33749599529 scopus 로고    scopus 로고
    • Impact of tropical storms and drought on the dinoflagellates Karlodinium micrum and Prorocentrum minimum in estuarine rivers of North Carolina, USA
    • FENSIN E.E. 2006. Impact of tropical storms and drought on the dinoflagellates Karlodinium micrum and Prorocentrum minimum in estuarine rivers of North Carolina, USA. African Journal of Marine Science 28: 277-281.
    • (2006) African Journal of Marine Science , vol.28 , pp. 277-281
    • FENSIN, E.E.1
  • 14
    • 2342541675 scopus 로고    scopus 로고
    • Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate)
    • GALLUZZI L., PENNA A., BERTOZZINI E., VILA M., GARCÉS E. & MAGNANI M. 2004. Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate). Applied and Environmental Microbiology 70: 1199-1206.
    • (2004) Applied and Environmental Microbiology , vol.70 , pp. 1199-1206
    • GALLUZZI, L.1    PENNA, A.2    BERTOZZINI, E.3    VILA, M.4    GARCÉS, E.5    MAGNANI, M.6
  • 15
    • 33748938547 scopus 로고    scopus 로고
    • GARCÉS E., FERNANDEZ M., PENNA A., LENNING K.V., GUTIERREZ A., CAMP J. & ZAPATA M. 2006. Characterization of NW Mediterranean Karlodinium spp. (Dinophyceae) strains using morphological, molecular, chemical, and physiological methodologies. Journal of Phycology 42: 1096-1112.
    • GARCÉS E., FERNANDEZ M., PENNA A., LENNING K.V., GUTIERREZ A., CAMP J. & ZAPATA M. 2006. Characterization of NW Mediterranean Karlodinium spp. (Dinophyceae) strains using morphological, molecular, chemical, and physiological methodologies. Journal of Phycology 42: 1096-1112.
  • 16
    • 0034860517 scopus 로고    scopus 로고
    • A second species of ichthyotoxic Pfiesteria (Dinamoebales, Dinophyceae)
    • GLASGOW H.B., BURKHOLDER J.M., MORTON S.L. & SPRINGER J. 2001. A second species of ichthyotoxic Pfiesteria (Dinamoebales, Dinophyceae). Phycologia 40: 234-245.
    • (2001) Phycologia , vol.40 , pp. 234-245
    • GLASGOW, H.B.1    BURKHOLDER, J.M.2    MORTON, S.L.3    SPRINGER, J.4
  • 17
    • 0141482157 scopus 로고    scopus 로고
    • Real-time PCR for quantification of Giardia and Cryptospordium in environmental water samples and sewage
    • GUY R.A., PAYMENT P., KRULL U.J. & HORGEN P.A. 2003. Real-time PCR for quantification of Giardia and Cryptospordium in environmental water samples and sewage. Applied and Environmental Microbiology 69: 5178-5185.
    • (2003) Applied and Environmental Microbiology , vol.69 , pp. 5178-5185
    • GUY, R.A.1    PAYMENT, P.2    KRULL, U.J.3    HORGEN, P.A.4
  • 19
    • 36849022010 scopus 로고    scopus 로고
    • Molecular detection of the brevetoxin-producing dinoflagellate Karenia brevis and closely related species using rRNA-targeted probes and a semiautomated sandwich hybridization assay
    • HAYWOOD A.J., SCHOLIN C.A., MARIN III R., STEIDINGER K.A., HEIL C. & RAY J. 2007. Molecular detection of the brevetoxin-producing dinoflagellate Karenia brevis and closely related species using rRNA-targeted probes and a semiautomated sandwich hybridization assay. Journal of Phycology 43: 1271-1286.
    • (2007) Journal of Phycology , vol.43 , pp. 1271-1286
    • HAYWOOD, A.J.1    SCHOLIN, C.A.2    MARIN III, R.3    STEIDINGER, K.A.4    HEIL, C.5    RAY, J.6
  • 20
    • 0035407042 scopus 로고    scopus 로고
    • Comparison of different approaches to quantify Staphylococcus aureus cells by real-time PCR and application of this technique for examination of cheeses
    • HEIN I., LEHNER A., RIECK P., KLEIN K., BRANDL E. & WAGNER M. 2001. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time PCR and application of this technique for examination of cheeses. Applied and Environmental Microbiology 67: 3122-3126.
    • (2001) Applied and Environmental Microbiology , vol.67 , pp. 3122-3126
    • HEIN, I.1    LEHNER, A.2    RIECK, P.3    KLEIN, K.4    BRANDL, E.5    WAGNER, M.6
  • 21
    • 0000504985 scopus 로고
    • A technique for counting chromosomes of armored dinoflagellates, and chromosome numbers of six freshwater dinoflagellate species
    • HOLT J.R. & PFIESTER L.A. 1982. A technique for counting chromosomes of armored dinoflagellates, and chromosome numbers of six freshwater dinoflagellate species. American Journal of Botany 69: 1165-1168.
    • (1982) American Journal of Botany , vol.69 , pp. 1165-1168
    • HOLT, J.R.1    PFIESTER, L.A.2
  • 22
    • 27644593262 scopus 로고    scopus 로고
    • Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by real-time PCR assay
    • HOSOI-TANABE S. & SAKO Y. 2005. Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by real-time PCR assay. Marine Biotechnology 7: 506-514.
    • (2005) Marine Biotechnology , vol.7 , pp. 506-514
    • HOSOI-TANABE, S.1    SAKO, Y.2
  • 23
    • 33947148224 scopus 로고    scopus 로고
    • Application of real-time PCR assay for detection and quantification of Alexandrium catenella cysts from marine sediments
    • KAMIKAWA R., NAGAI S., HOSOI-TANABE S., ITAKURA S., YAMAGUCHI M., UCHIDA Y., BABA T. & SAKO Y. 2007. Application of real-time PCR assay for detection and quantification of Alexandrium catenella cysts from marine sediments. Harmful Algae 6: 413-420.
    • (2007) Harmful Algae , vol.6 , pp. 413-420
    • KAMIKAWA, R.1    NAGAI, S.2    HOSOI-TANABE, S.3    ITAKURA, S.4    YAMAGUCHI, M.5    UCHIDA, Y.6    BABA, T.7    SAKO, Y.8
  • 24
    • 0012849526 scopus 로고    scopus 로고
    • Toxicity of Karlodinium micrum (Dinophyceae) associated with a fish kill in a South Carolina brackish retention pond
    • KEMPTON J.W., LEWITUS A.J., DEEDS J.R., LAW J.M. & PLACE A.R. 2002. Toxicity of Karlodinium micrum (Dinophyceae) associated with a fish kill in a South Carolina brackish retention pond. Harmful Algae 1: 233-241.
    • (2002) Harmful Algae , vol.1 , pp. 233-241
    • KEMPTON, J.W.1    LEWITUS, A.J.2    DEEDS, J.R.3    LAW, J.M.4    PLACE, A.R.5
  • 25
    • 33745712218 scopus 로고    scopus 로고
    • Low abundance distribution of Pfiesteria piscicida in Pacific and Western Atlantic as detected by mtDNA-18S rDNA real-time polymerase chain reaction
    • LIN S., ZHANG H. & DUBOIS A. 2006. Low abundance distribution of Pfiesteria piscicida in Pacific and Western Atlantic as detected by mtDNA-18S rDNA real-time polymerase chain reaction. Journal of Plankton Research 28: 667-681.
    • (2006) Journal of Plankton Research , vol.28 , pp. 667-681
    • LIN, S.1    ZHANG, H.2    DUBOIS, A.3
  • 31
    • 34247104316 scopus 로고    scopus 로고
    • MASON P.L., LITAKER R.W., JEONG H.J., HA J.H., REECE K.S., STOKES N.A., PARK J.Y., STEIDINGER K.A., VANDERSEA M.W., KIBLER S., TESTER P.A. & VOGELLBEIN W.K. 2007. Description of a new genus of Pfiesteria-like dinoflagellate, Luciella gen. nov. (Dinophyceae), including two new species: Luciella masanensis sp. nov. and Luciella atlantis sp. nov. Journal of Phycology 43: 799-810.
    • MASON P.L., LITAKER R.W., JEONG H.J., HA J.H., REECE K.S., STOKES N.A., PARK J.Y., STEIDINGER K.A., VANDERSEA M.W., KIBLER S., TESTER P.A. & VOGELLBEIN W.K. 2007. Description of a new genus of Pfiesteria-like dinoflagellate, Luciella gen. nov. (Dinophyceae), including two new species: Luciella masanensis sp. nov. and Luciella atlantis sp. nov. Journal of Phycology 43: 799-810.
  • 32
    • 30944451346 scopus 로고    scopus 로고
    • Nucleic acid amplification-based techniques for pathogen detection and identification
    • MONIS P.T. & GIGLIO S. 2006. Nucleic acid amplification-based techniques for pathogen detection and identification. Infection Genetics Evolution 6: 2-12.
    • (2006) Infection Genetics Evolution , vol.6 , pp. 2-12
    • MONIS, P.T.1    GIGLIO, S.2
  • 33
    • 15544371286 scopus 로고    scopus 로고
    • Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis
    • MONIS P.T., GIGLIO S. & SAINT C.P. 2005. Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Analytical Biochemistry 370: 24-34.
    • (2005) Analytical Biochemistry , vol.370 , pp. 24-34
    • MONIS, P.T.1    GIGLIO, S.2    SAINT, C.P.3
  • 34
    • 33748447166 scopus 로고    scopus 로고
    • Use of quantitative real-time PCR to investigate the dynamics of the red tide dinoflagellate Lingulodinium polyedrum
    • MOORTHI S.D., COUNTWAY P.D., STAUFFER B.A. & CARON D.A. 2006. Use of quantitative real-time PCR to investigate the dynamics of the red tide dinoflagellate Lingulodinium polyedrum. Microbial Ecology 52: 136-150.
    • (2006) Microbial Ecology , vol.52 , pp. 136-150
    • MOORTHI, S.D.1    COUNTWAY, P.D.2    STAUFFER, B.A.3    CARON, D.A.4
  • 35
    • 0034636108 scopus 로고    scopus 로고
    • OLDACH D.W., DELWICHE C.F., JAKOBSEN K.S., TENGS T., BROWN E.G., KEMPTON J.W., SCHAEFER E.F., BOWERS H., STEIDINGER K., GLASGOW H.B. JR, BURKHOLDER J.M. & RUBLEE P.A. 2000. Heteroduplex mobility assay guided sequence discovery: elucidation of the small subunit (18S) rDNA sequence of Pfiesteria piscicida from complex algal culture and environmental sample DNA pools. Proceedings of the National Academy of Sciences of the United States of America 97: 4303-4308.
    • OLDACH D.W., DELWICHE C.F., JAKOBSEN K.S., TENGS T., BROWN E.G., KEMPTON J.W., SCHAEFER E.F., BOWERS H., STEIDINGER K., GLASGOW H.B. JR, BURKHOLDER J.M. & RUBLEE P.A. 2000. Heteroduplex mobility assay guided sequence discovery: elucidation of the small subunit (18S) rDNA sequence of Pfiesteria piscicida from complex algal culture and environmental sample DNA pools. Proceedings of the National Academy of Sciences of the United States of America 97: 4303-4308.
  • 36
    • 34548024372 scopus 로고    scopus 로고
    • Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates
    • PARK T.G., BOLCH C.J.S. & HALLEGRAEFF G.M. 2007a. Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates. Harmful Algae 6: 718-733.
    • (2007) Harmful Algae , vol.6 , pp. 718-733
    • PARK, T.G.1    BOLCH, C.J.S.2    HALLEGRAEFF, G.M.3
  • 37
    • 34247605605 scopus 로고    scopus 로고
    • Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples
    • PARK T.G., DE SALAS, M.F., BOLCH C.J.S. & HALLEGRAEFF G.M. 2007b. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Applied and Environmental Microbiology 73: 2552-2560.
    • (2007) Applied and Environmental Microbiology , vol.73 , pp. 2552-2560
    • PARK, T.G.1    DE SALAS, M.F.2    BOLCH, C.J.S.3    HALLEGRAEFF, G.M.4
  • 38
    • 0041402648 scopus 로고    scopus 로고
    • Estuarine heterotrophic eryptoperidiniopsoids (Dinophyceae): Life cycle and culture studies
    • PARROW M.W. & BURKHOLDER J.M. 2003. Estuarine heterotrophic eryptoperidiniopsoids (Dinophyceae): life cycle and culture studies. Journal of Phycology 39: 678-696.
    • (2003) Journal of Phycology , vol.39 , pp. 678-696
    • PARROW, M.W.1    BURKHOLDER, J.M.2
  • 39
    • 85009436486 scopus 로고    scopus 로고
    • PLACE A.R., SAITO K., DEEDS J.R., ROBLEDO J.A.F. & VASTA G.R. 2008. A decade of research on Pfiesteria spp. and their toxins: unresolved questions and an alternative hypothesis. In: Seafood and freshwater toxins (Ed. by LuisM. Botana), pp. 717-751. CRC Press, New York.
    • PLACE A.R., SAITO K., DEEDS J.R., ROBLEDO J.A.F. & VASTA G.R. 2008. A decade of research on Pfiesteria spp. and their toxins: unresolved questions and an alternative hypothesis. In: Seafood and freshwater toxins (Ed. by LuisM. Botana), pp. 717-751. CRC Press, New York.
  • 41
    • 33748789306 scopus 로고    scopus 로고
    • Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis
    • ROBINSON B.S., MONIS P.T. & DOBSON P.J. 2006. Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Applied and Environmental Microbiology 72: 5857-5863.
    • (2006) Applied and Environmental Microbiology , vol.72 , pp. 5857-5863
    • ROBINSON, B.S.1    MONIS, P.T.2    DOBSON, P.J.3
  • 42
    • 21844453446 scopus 로고    scopus 로고
    • Detection of the dinozoans Pfiesteria piscicida and P. shumwayae: A review of detection methods and geofraphic distribution
    • RUBLEE P.M., REMINGTON D.L., SCHAEFER E.F. & MARSHALL M.M. 2005. Detection of the dinozoans Pfiesteria piscicida and P. shumwayae: a review of detection methods and geofraphic distribution. Journal of Eukaryotic Microbiology 52: 83-89.
    • (2005) Journal of Eukaryotic Microbiology , vol.52 , pp. 83-89
    • RUBLEE, P.M.1    REMINGTON, D.L.2    SCHAEFER, E.F.3    MARSHALL, M.M.4
  • 43
    • 33746296843 scopus 로고    scopus 로고
    • Pfiesteria piscicida and Pfiesteria shumwayae in coastal waters of Long island. New York USA
    • RUBLEE P.M., NUZZI R., WATERS R., SCHAEFER E.F. & BURKHOLDER J.M. 2006. Pfiesteria piscicida and Pfiesteria shumwayae in coastal waters of Long island. New York USA. Harmful Algae 5: 374-379.
    • (2006) Harmful Algae , vol.5 , pp. 374-379
    • RUBLEE, P.M.1    NUZZI, R.2    WATERS, R.3    SCHAEFER, E.F.4    BURKHOLDER, J.M.5
  • 44
    • 0036840289 scopus 로고    scopus 로고
    • Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the non-transcribed spacer
    • SAITO K., DRGON T., ROBLEDO J.A.F., KRUPATKINA D.N. & VASTA G.R. 2002. Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the non-transcribed spacer. Applied and Environmental Microbiology 68: 5394-5407.
    • (2002) Applied and Environmental Microbiology , vol.68 , pp. 5394-5407
    • SAITO, K.1    DRGON, T.2    ROBLEDO, J.A.F.3    KRUPATKINA, D.N.4    VASTA, G.R.5
  • 45
    • 8744263850 scopus 로고    scopus 로고
    • Real-time quantitative PCR: A new technology to detect and study phytopathogenic and antagonistic fungi
    • SCHENA L., NIGRO F., IPPOLITO A. & GALLITELLI D. 2004. Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. European Journal of Plant Pathology 110: 893-908.
    • (2004) European Journal of Plant Pathology , vol.110 , pp. 893-908
    • SCHENA, L.1    NIGRO, F.2    IPPOLITO, A.3    GALLITELLI, D.4
  • 46
    • 33746403711 scopus 로고    scopus 로고
    • Cryptoperidiniopsis brodyi gen. Et sp. nov. (Dinophyceae) a small lightly armored dinoflagellate in the Pfiesteriaccae
    • STEIDINGER K.A., LANDSBERG J.H., MASON P.L., VOGELBEIN W.K., TESTER P.A. & LITAKER R.W. 2006. Cryptoperidiniopsis brodyi gen. Et sp. nov. (Dinophyceae) a small lightly armored dinoflagellate in the Pfiesteriaccae. Journal of Phycology 42: 951-961.
    • (2006) Journal of Phycology , vol.42 , pp. 951-961
    • STEIDINGER, K.A.1    LANDSBERG, J.H.2    MASON, P.L.3    VOGELBEIN, W.K.4    TESTER, P.A.5    LITAKER, R.W.6
  • 47
    • 0031574072 scopus 로고    scopus 로고
    • The CLUSTALX windows interface: Flexible strategies for multiple sequence alignment aided by quality tools
    • THOMPSON J.D., GIBSON T.J., PLEWNAIK F., JEANMOUGIN F. & HIGGINS D.G. 1997. The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality tools. Nucleic Acids Research 25: 4876-4882.
    • (1997) Nucleic Acids Research , vol.25 , pp. 4876-4882
    • THOMPSON, J.D.1    GIBSON, T.J.2    PLEWNAIK, F.3    JEANMOUGIN, F.4    HIGGINS, D.G.5
  • 48
    • 0036116388 scopus 로고    scopus 로고
    • Quantifying the phenolic content of frcshwaters using simple assays with different underlying reaction mechanisms
    • THOSS V., BAIRD M.S., LOCK M.A. & COURTY P.V. 2002. Quantifying the phenolic content of frcshwaters using simple assays with different underlying reaction mechanisms. Journal of Environmental Monitoring 4: 270-275.
    • (2002) Journal of Environmental Monitoring , vol.4 , pp. 270-275
    • THOSS, V.1    BAIRD, M.S.2    LOCK, M.A.3    COURTY, P.V.4
  • 49
    • 34250882734 scopus 로고    scopus 로고
    • Faster, simplier, more-specific methods for improved molecular detection of Phytophthora ramorum in the field
    • TOMLINSON J.A., BARKER I. & BOONHAM N. 2007. Faster, simplier, more-specific methods for improved molecular detection of Phytophthora ramorum in the field. Applied and Environmental Microbiology 73: 4040-4047.
    • (2007) Applied and Environmental Microbiology , vol.73 , pp. 4040-4047
    • TOMLINSON, J.A.1    BARKER, I.2    BOONHAM, N.3
  • 50
    • 0037134017 scopus 로고    scopus 로고
    • Tech. Sight. A technique whose time has come
    • WALKER N.J. 2002. Tech. Sight. A technique whose time has come. Science 296: 557-559.
    • (2002) Science , vol.296 , pp. 557-559
    • WALKER, N.J.1
  • 51
    • 0030748867 scopus 로고    scopus 로고
    • Inhibition and facilitation of nucleic acid amplification
    • WILSON I.G. 1997. Inhibition and facilitation of nucleic acid amplification. Applied and Environmental Microbiology 63: 3741-3751.
    • (1997) Applied and Environmental Microbiology , vol.63 , pp. 3741-3751
    • WILSON, I.G.1
  • 53
    • 32044446476 scopus 로고    scopus 로고
    • Development of a cob-18S rDNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment
    • ZHANG H. & LIN S. 2005. Development of a cob-18S rDNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment. Applied and Environmental Microbiology 71: 7053-7063.
    • (2005) Applied and Environmental Microbiology , vol.71 , pp. 7053-7063
    • ZHANG, H.1    LIN, S.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.