Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by real-time PCR assay

Marine Biotechnology

Volumn 7, Issue 5, 2005, Pages 506-514

  Hosoi Tanabe, Shoko ^a,b   Sako, Yoshihiko ^a  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIGA PREFECTURE   (Japan)

Author keywords

Alexandrium; Paralytic shellfish poisoning (PSP); Quantification; Real time PCR; Species specific identification; Toxic dinoflagellate

Indexed keywords

ALGAE; AMPLIFICATION; BIODIVERSITY; DNA; OPTICAL MICROSCOPY;

ALEXANDRIUM; PARALYTIC SHELLFISH POISONING (PSP); QUANTIFICATION; REAL-TIME PCR; SPECIES-SPECIFIC IDENTIFICATION; TOXIC DINOFLAGELLATE;

REAL TIME SYSTEMS;

PRIMER DNA; PROTOZOAL DNA; RIBOSOME DNA; RNA 28S;

DETECTION METHOD; DINOFLAGELLATE; PARALYTIC SHELLFISH POISONING; POLYMERASE CHAIN REACTION; TOXIC ORGANISM;

ANIMAL; ARTICLE; CELL COUNT; CLASSIFICATION; COMPARATIVE STUDY; DINOFLAGELLATE; DNA PROBE; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE;

ANIMALS; CELL COUNT; DINOFLAGELLIDA; DNA PRIMERS; DNA PROBES; DNA, PROTOZOAN; DNA, RIBOSOMAL; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 28S; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

ALEXANDRIUM; ALEXANDRIUM CATENELLA; ALEXANDRIUM TAMARENSE; DINOPHYCEAE;

EID: 27644593262     PISSN: 14362228     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10126-004-4128-4     Document Type: Article

References (26)

1. Adachi M, Sako Y, Ishida Y (1994) Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae). J Phycol 30, 857-863
2. Adachi M, Sako Y, Ishida Y (1996) Identification of the toxic dinoflagellates Alexandrium catenella and A. tamarense (Dinophyceae) using DNA probes and whole-cell hybridization. J Phycol 32, 1049-1052
3. Balech E (1995) The Genus Alexandrium Halim (Dinoflagellate). (Cork, Ireland: Sherwin Island Press)
4. Bowers HA, Tengs T, Glasgow HB Jr, Burkholder JM, Rublee PA, Oldach DW (2000) Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Appl Environ Microbiol 66, 4641-4648
5. Chen LCM, Edelstein T, McLachlan J (1969) Bonnemaisonia hamifera Hariot in nature and in culture. J Phycol 5, 211-220
6. Cocolin L, Manzano M, Cantoni C, Comi G (1998) Use of polymerase chain reaction and restriction enzyme analysis to directly detect and identify Salmonella typhimurium in food. J Appl Microbiol 85, 673-677
7. Galluzzi L, Penna A, Bertozzini E, Vila M, Garces E, Magnani M (2004) Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate). Appl Environ Microbiol 70, 1199-1206
8. Guiver M, Levi K, Oppenheim BA (2001) Rapid identification of Candida species by Taq Man PCR. J Clin Pathol 54, 362-366
9. Heley ST, Cavender JF, Murray TE (1999) Detection of Alexandrium tamarensis by rapid PCR analysis. Biotechques 26, 88-91
10. Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Nacl Acad Sci U S A 88, 7276-7280
11. Lee LG, Connell CR, Bloch W (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nuceleic Acids Res 21, 3761-3766
12. Lenaers G, Scholin CA, Bhaud Y, Saint-Hilarie D, Herzog M (1991) A molecular phylogeny of dinoflagellate protists (Pyrrophyta) inferred from the sequence of 24S ribosomal DNA divergent domains D1 and D8. J Mol Evol 32, 53-63
13. Lofstrom C, Knutsson R, Axelsson CE, Radstrom P (2004) Rapid and specific detection of Salmonella spp. in animal feed samples by PCR after culture enrichment. Appl Environ Microbiol 70, 69-75
14. Mauchline TH, Kerry BR, Hirsch PR (2002) Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Appl Environ Microbiol 68, 1846-1853
15. Muller JR, Janz S, Goedert JJ, Potter M, Rabkin CS (1995) Persistence of immunoglobulin heavy chain/c-myc recombination-positive lymphocyte clones in the blood of human immunodeficiency virus-infected homosexual men. Proc Natl Acad Sci U S A 92, 6577-6581
16. Oberst RD, Hays MP, Bohra LK, Phebus RK, Yamahiro CT, Paszko-Kolva C, Flood SJA, Sargeant JM, Gillespie JR (1998) PCR-based DNA amplification and presumptive detection of Escherichia coli O157 : H7 with an internal fluorogenic probe and the 5′ nuclease (Taq Man) assay. Appl Environ Microbiol 64, 3389-3396
17. Penna A, Magnani M (1999) Identification of Alexandrium (Dinophyceae) species using PCR and rDNA-targeted probes. J Phycol 35, 615-621
18. Penna A, Magnani M (2000) A PCR immunoassay method for the detection of Alexandrium (Dinophyceae) species. J Phycol 36, 1183-1186
19. Sako Y, Kim C-H, Ninomiya H, Adachi M, Ishida Y (1990) Isozyme and cross analysis of mating population in the Alexandrium catenella / tamarense species complex. In: Toxic Marine Phytoplankton, Graneli E, Sundstrom B, Edler L, Anderson DM, eds. (NewYork, N.Y.: Elsevier) 320-323
20. Sako Y, Hosoi-Tanabe S, Uchida (2004) Fluorescence in situ hybridization using rRNA-targeted probes for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and A. catenella. J Phycol 40, 598-605
21. Schantz EJ, Lynch JM, Vayvanda G, Matsumoto K, Rapoport H (1966) The purification and characterization of the poison produced by Gonyaulax catenella in axenic culture. Biochemistry 5, 1191-1195
22. Scholin CA, Herzog M, Sogin M, Anderson DM (1994) Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae), II: sequences analysis of a fragment of the LSU rRNA gene. J Phycol 30, 999-1011
23. Sharma VK, Carlson SA (2000) Simultaneous detection of Salmonella strains and Escherichia coli O157:H7 with fluorogenic PCR and single-enrichment-broth culture. Appl Environ Microbiol 66, 5472-5476
24. Shin JH, Nolte FS, Holloway BP, Morrison CJ (1999) Rapid identification of up to three Candida species in a single reaction tube by a 5′ exonuclease assay using fluorescent DNA probes. J Clin Microbial 37, 165-170
25. Shumway SE (1990) A review of the effects of algal blooms on shellfish and aquaculture. J World Aquacult Soc 21, 65-104
26. Zhang H, Lin S (2002) Detection and quantification of Pfiesteria piscicida by using the mitochondrial cytochrome b gene. Appl Environ Microbiol 68, 989-994