Molecular quantification of toxic Alexandrium fundyense in the Gulf of Maine using real-time PCR

Harmful Algae

Volumn 5, Issue 3, 2006, Pages 242-250

  Dyhrman, Sonya T ^a   Erdner, Deana ^a   Du, Jane La ^a   Galac, Madeline ^a   Anderson, Donald M ^a  

^a WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

Author keywords

Alexandrium; Gulf of Maine; Paralytic shellfish poisoning; qPCR

Indexed keywords

ALEXANDRIUM; ALEXANDRIUM FUNDYENSE; ALEXANDRIUM OSTENFELDII; ALGAE; DINOPHYCEAE;

EID: 33644852180     PISSN: 15689883     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.hal.2005.07.005     Document Type: Article

References (30)

1. D.M. Anderson Bloom dynamics of toxic Alexandrium species in the northeastern U.S. Limnol. Oceanogr. 42 1997 1009 1022
2. D.M. Anderson, D.M. Kulis, G.J. Doucette, J.C. Gallagher, and E. Balech Biogeography of toxic dinoflagellates in the genus Alexandrium from the northeastern United States and Canada Mar. Biol. 120 1994 467 478
3. D.M. Anderson, D.M. Kulis, B.A. Keafer, and E. Berdalet Detection of the toxic dinoflagellate Alexandrium fundyense (Dinophyceae) with oligonucleotides and antibody probes: variability in labelling intensity with physiological condition J. Phycol. 35 1999 870 883
4. Anderson, D.M., Kulis, D.M., Keafer, B.A., Gribble, K.E., Marin, R., Scholin, C.A. Identification and enumeration of Alexandrium spp. from the Gulf of Maine using molecular probes. Deep Sea Research II, in press.
5. H. Bowers, T. Tengs, H. Glasgow, J. Burkholder, P. Rublee, and D. Oldach Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates Appl. Environ. Microbiol. 66 2000 4641 4648
6. E.T. Casper, J.H. Paul, M.C. Smith, and M. Gray Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification Appl. Environ. Microbiol. 70 2004 4727 4732
7. Coyne, K.J., Handy, S.M., Demir, E., Whereat, E.B., Hutchins, D.A., Portune, K.J., Doblin, M.A., Cary, S.C. Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnol. Oceanogr.: Methods, in press.
8. P.F. Culverhouse, R. Williams, B. Reguera, V. Henry, and S. Gonzalez-Gil Do experts make mistakes? A comparison of human and machine identification of dinoflagellates Mar. Ecol. Prog. Ser. 247 2003 17 25
9. S.T. Dyhrman, and D.M. Anderson Urease activity in cultures and field populations of the toxic dinoflagellate Alexandrium Limnol. Oceanogr. 48 2003 647 655
10. W.M. Freeman, S.J. Walker, and K.E. Vrana Quantitative RT-PCR: pitfalls and potential Biotechniques 26 1999 112 125
11. L. Galluzzi, A. Penna, E. Bertozzini, M. Vila, E. Garces, and M. Magnani Development of a real-time assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate) Appl. Environ. Microbiol. 70 2004 1199 1206
12. M. Gray, B. Wawrik, J. Paul, and E. Casper Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment Appl. Environ. Microbiol. 69 2003 5726 5730
13. Gribble, K.E., Keafer, B.A., Quilliam, M., Cembella, A.D., Kulis, D.M., Manahan, A., Anderson, D.M. Distribution and toxicity of Alexandrium ostenfeldii in the Gulf of Maine, USA. Deep Sea Research II, in press.
14. R.R.L. Guillard Culture of phytoplankton for feeding marine invertebrates W.L. Smith M.H. Chanley Culture of Marine Invertebrate Animals 1975 Plenum New York 29 60
15. G.M. Hallegraeff A review of harmful algal blooms and their apparent increase Phycologia 32 1993 79 99
16. W.A. Higman, D.M. Stone, and J.M. Lewis Sequence comparisons of toxic and non-toxic Alexandrium tamarense (Dinophyceae) isolates from UK waters Phycologia 40 2001 256 262
17. P. Hoagland, D.M. Anderson, Y. Kaoru, and A.W. White The economic effects of harmful algal blooms in the United States: estimates, assessment issues, and information needs Estuaries 25 2002 819 837
18. U. John Discrimination of the toxigenic dinoflagellates Alexandrium tamarense and A. ostenfeldii in co-occurring natural populations from Scottish coastal waters Eur. J. Phycol. 38 2003 25 40
19. L.K. Medlin, M. Lange, U. Wellbrock, G. Donner, M. Elbrächter, C. Hummert, and B. Luckas Sequence comparisons link toxic European isolates of A. tamarense from the Orkney Islands to toxic North American stocks Eur. J. Protistol. 34 1998 329 335
20. A. Penna, and M. Magnani Identification of Alexandrium (Dinophyceae) species using PCR and rDNA-targeted probes J. Phycol. 35 1999 615 621
21. A. Penna, and M. Magnani A PCR immunoassay method for the detection of Alexandrium (Dinophyceae) species J. Phycol. 36 2000 1183 1186
22. M.W. Pfaffl A new mathematical model for relative quantification for real-time RT-PCR Nucleic Acids Res. 29 2001 2002 2007
23. L.C. Popels, S.C. Cary, D.A. Hutchins, R. Forbes, F. Pustizzi, C.J. Gobler, and K.J. Coyne The use of quantitative polymerase chain reaction for the detection and enumeration of the harmful alga Aureococcus anophagefferens in environmental samples along the United States East Coast Limnol. Oceanogr.: Methods 1 2003 92 102
24. K. Saito, T. Drgon, J.A. Robledo, D.N. Krupatkina, and G. Vasta Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the nontranscribed spacer Appl. Environ. Microbiol. 68 2002 5394 5407
25. C.A. Scholin, M. Herzog, M. Sogin, and D.M. Anderson Identification of group- and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the large subunit ribosomal RNA gene J. Phycol. 30 1994 999 1011
26. C.A. Scholin, and D.M. Anderson Detection and quantification of HAB species using antibody and DNA probes: progress to date and future objectives B. Reguera J. Blanco M.L. Fernandez T. Wyatt Harmful Algae 1998 IOC UNESCO Paris 253 257
27. C.A. Scholin, K.R. Buck, T. Britschgi, G. Cangelosi, and F.P. Chavez Identification of Pseudo-nitzschia australis (Bacillariophyceae) using rRNA-targeted probes in whole cell and sandwich hybridization formats Phycologia 35 1996 190 197
28. C.A. Scholin, G.M. Hallegraeff, and D.M. Anderson Molecular evolution of the Alexandrium tamarense 'species complex' (Dinophyceae): dispersal in the North American and west Pacific regions Phycologia 34 1995 472 485
29. H. Utermöhl Zur vervolkommung der quantitativenphytoplankton- methodik Mitt. Int. Ver. Theor. Angew. Limnol. 9 1958 1 38
30. F. Van Dolah Marine algal toxins: origins, health effects, and their increased occurrence Environ. Health Perspect. 108 2000 133 141