Analysis of the human casein phosphoproteome by 2-D electrophoresis and MALDI-TOF/TOF MS reveals new phosphoforms

Journal of Proteome Research

Volumn 7, Issue 11, 2008, Pages 5017-5027

  Poth, Aaron G ^a   Deeth, Hilton C ^a   Alewood, Paul F ^a   Holland, John W ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

s1 casein; casein; Deamidation; Human milk; Phosphorylation

Indexed keywords

ALPHA CASEIN; BETA CASEIN; PHOSPHATE; PHOSPHOPEPTIDE; CASEIN; ISOPROTEIN; PROTEOME;

ARTICLE; BREAST MILK; CONTROLLED STUDY; DEAMINATION; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; CHEMISTRY; GENETICS; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; PHOSPHORYLATION;

MAMMALIA;

AMINO ACID SEQUENCE; CASEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; MILK, HUMAN; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN ISOFORMS; PROTEOME; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 58149394487     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr800387s     Document Type: Article

References (34)

1. Clare, D. A.; Swaisgood, H. E. Bioactive milk peptides: a prospectus. J. Dairy Sci. 2000, 83, 1187-1195.
2. Meisel, H. Biochemical properties of peptides encrypted in bovine milk proteins. Curr. Med. Chem. 2005, 12, 1905-19.
3. Meisel, H.; FitzGerald, R. J. Biofunctional peptides from milk proteins: mineral binding and cytomodulatory effects. Curr. Pharm. Des. 2003, 9, 1289-95.
4. Silva, S. V.; Malcata, F. X. Caseins as source of bioactive peptides. Int. Dairy J. 2005, 15, 1-15.
5. Cox, D. M.; Zhong, F.; Du, M.; Duchoslav, E.; Sakuma, T.; McDermott, J. C. Multiple reaction monitoring as a method for identifying protein posttranslational modifications. J. BiomoL Tech. 2005, 16, 83-90.
6. Kapkova, P.; Lattova, E.; Perreault, H. Nonretentive solid-phase extraction of phosphorylated peptides from complex peptide mixtures for detection by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 2006, 78, 7027-33.
7. Sweet, S. M. M.; Creese, A. J.; Cooper, H. J. Strategy for the identification of sites of phosphorylation in proteins: neutral loss triggered electron capture dissociation. Anal. Chem. 2006, 78, 7563-7569.
8. Wu, H. Y.; Tseng, V. S. M.; Liao, P. C. Mining phosphopeptide signals in liquid chromatography-mass spectrometry data for protein phosphorylation analysis. J. Proteome Res. 2007, 6, 1812- 1821.
9. Zhou, H.; Xu, S.; Ye, M.; Feng, S.; Pan, C; Jiang, X.; Li, X.; Han, G.; Fu, Y.; Zou, H. Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis. J. Proteome Res. 2006, 5, 2431-7.
10. Farrell, H. M., Jr.; Jimenez-Flores, R.; Bleck, G. T.; Brown, E. M.; Butler, J. E.; Creamer, L. K.; Hicks, C. L.; Hollar, C. M.; Ng-Kwai-Hang, K. F.; Swaisgood, H. E. Nomenclature of the proteins of cows' milk-sixth revision. J. Dairy Sci. 2004, 87, 1641-1674.
11. s1, β-, κ-caseins, γ-casein-like, and proteose peptone component 5-like peptides. J. Dairy Sci. 2002, 85, 697-706.
12. Ferranti, P.; Pizzano, R.; Garro, G.; Caira, S.; Chianese, L.; Addeo, F. Mass spectrometry-based procedure for the identification of ovine casein heterogeneity. J. Dairy Res. 2001, 68, 35-51.
13. Ginger, M. R.; Piotte, C. P.; Otter, D. E.; Grigor, M. R. Identification, characterisation and cDNA cloning of two caseins from the common brush tail possum (Trichosurus vulpecula). Biochim. Biophys. Acta 1999, 1427, 92-104.
14. Home, D. S.; Anema, S.; Zhu, X.; Nicholas, K. R.; Singh, H. A lactational study of the composition and integrity of casein micelles from the milk of the tammar wallaby (Macropus eugenii). Arch. Biochem. Biophys. 2007, 467, 107-118.
15. Greenberg, R.; Groves, M. L.; Dower, H. J. Human β-casein. Amino acid sequence and identification of phosphorylation sites. J. Biol. Chem. 1984, 259, 5132-5138.
16. s1-casein is markedly different from the ruminant species. Eur. J. Biochem. 2003, 270, 3651-3655.
17. Kjeldsen, F.; Savitski, M. M.; Nielsen, M. L.; Shi, L.; Zubarev, R. A. On studying protein phosphorylation patterns using bottom-up LC-MS/MS: the case of human a-casein. Analyst 2007, 132, 768-76.
18. Steen, H.; Jebanathirajah, J. A.; Rush, J.; Morrice, N.; Kirschner, M. W. Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements. Mol. Cell. Proteomics 2006, 5, 172-181.
19. Bodenmiller, B.; Mueller, L. N.; Mueller, M.; Domon, B.; Aebersold, R. Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Nat. Methods 2007, 4, 231-237.
20. Girardet, J.-M.; Miclo, L.; Florent, S.; Mollé, D.; Gaillard, J.-L. Determination of the phosphorylation level and deamidation susceptibility of equine βcasein. Proteomics 2006, 6, 3707-3717.
21. Kunz, C; Lonnerdal, B. Human milk proteins: separation of whey proxeins and their analysis by polyacrylamide gel electrophoresis, fast protein liquid chromatography (FPLC) gel filtration, and anion-exchange chromatography. Am. J. Clin. Nutr. 1989, 49, 464-470.
22. Holland, J. W.; Deeth, H. C; Alewood, P. F. Resolution and characterisation of multiple isoforms of bovine κ-casein by 2-DE following a reversible cysteine-tagging enrichment strategy. Proteomics 2006, 6, 3087-95.
23. Kjellstrom, S.; Jensen, O. N. Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins. Anal. Chem. 2004, 76, 5109-5117.
24. Stensballe, A.; Jensen, O. N. Phosphoric acid enhances the performance of Fe(HI) affinity chromatography and matrix-assisted laser desorption/ionization tandem mass spectrometry for recovery, detection and sequencing of phosphopeptides. Rapid Commun. Mass Spectrom. 2004, 18, 1721-1730.
25. Lasa-Benito, M.; Marin, O.; Meggio, F.; Pinna, L. A. Golgi apparatus mammary gland casein kinase: monitoring by a specific peptide substrate and definition of specificity determinants. FEBS Lett. 1996, 382, 149-52.
26. Mercier, J. C. Phosphorylation of caseins, present evidence for an amino acid triplet code posttranslationally recognized by specific kinases. Biochimie 1981, 63, 1-17.
27. Boyle, W. J.; van der Geer, P.; Hunter, T. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates. Methods Enzymol. 1991, 201, 110-149.
28. Schlosser, A.; Pipkorn, R.; Bossemeyer, D.; Lehmann, W. D. Analysis of protein phosphorylation by a combination of elastase digestion and neutral loss tandem mass spectrometry. Anal. Chem. 2001, 73, 170-176.
29. Wright, H. T. Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins. Git Rev. Biochem. Mol. Biol 1991, 26, 1-52.
30. Ferranti, P.; Traisci, M. V.; Picariello, G.; Nasi, A.; Boschi, V.; Siervo, M.; Falconi, C; Chianese, L.; Addeo, F. Casein proteolysis in human milk: tracing the pattern of casein breakdown and the formation of potential bioactive peptides. J. Dairy Res. 2004, 71, 74-87.
31. McMahon, D. J.; Oommen, B. S. Supramolecular Structure of the Casein Micelle. J. Dairy Sci. 2008, 91, 1709-1721.
32. Home, D. S. Casein micelle structure: Models and muddles. Curr. Opin. Colloid Interface Sci. 2006, 11, 148-153.
33. Farrell, J. H. M.; Malin, E. L.; Brown, E. M.; Qi, P. X. Casein micelle structure: What can be learned from milk synthesis and structural biology. Curr. Opin. Colloid Interface Sci. 2006, 11, 135-147.
34. Lonnerdal, B. Nutritional and physiologic significance of human milk proteins. Am. I. Clin. Nutr. 2003, 77, 1537S-1543S.