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Volumn 64, Issue 1, 2009, Pages 1-7

A novel, generic and effective method for the rapid purification of G protein-coupled receptors

Author keywords

Cannabinoid; G protein coupled receptor; Ligand; Opioid; Pichia pastoris; Purification; Solubilization

Indexed keywords

G PROTEIN COUPLED RECEPTOR; HYBRID PROTEIN; MICROSPHERE; PROTEINASE; SEPHAROSE; STREPTAVIDIN; TEV PROTEASE;

EID: 57649102148     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.09.007     Document Type: Article
Times cited : (11)

References (25)
  • 1
    • 0038024615 scopus 로고    scopus 로고
    • The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints
    • Fredriksson R., Lagerström M.C., Lundin L.-G., and Schiöth H.B. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol. Pharmacol. 63 (2003) 1256-1272
    • (2003) Mol. Pharmacol. , vol.63 , pp. 1256-1272
    • Fredriksson, R.1    Lagerström, M.C.2    Lundin, L.-G.3    Schiöth, H.B.4
  • 2
    • 4344581120 scopus 로고    scopus 로고
    • The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure
    • Okada T., Sugihara M., Bondar A.N., Elstner M., Entel P., and Buss V. The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure. J. Mol. Biol. 342 (2004) 571-583
    • (2004) J. Mol. Biol. , vol.342 , pp. 571-583
    • Okada, T.1    Sugihara, M.2    Bondar, A.N.3    Elstner, M.4    Entel, P.5    Buss, V.6
  • 5
    • 34250636733 scopus 로고    scopus 로고
    • 2-adrenergic receptor expressed in methylotrophic yeast Pichia pastoris
    • 2-adrenergic receptor expressed in methylotrophic yeast Pichia pastoris. J. Biochem. 14 (2006) 799-804
    • (2006) J. Biochem. , vol.14 , pp. 799-804
    • Noguchi, S.1    Satow, Y.2
  • 6
    • 0031610110 scopus 로고    scopus 로고
    • H.M. Weiß, W. Haase, H. Reiländer, Expression of an integral membrane protein, in: Methods in Molecular Biology, Pichia Protocols, vol. 103, Humana press, 1998, pp 227-239.
    • H.M. Weiß, W. Haase, H. Reiländer, Expression of an integral membrane protein, in: Methods in Molecular Biology, Pichia Protocols, vol. 103, Humana press, 1998, pp 227-239.
  • 7
    • 33747780439 scopus 로고    scopus 로고
    • Expression of a glycosylation deficient version of the human adenosine 2a receptor for structural studies
    • Fraser N.J. Expression of a glycosylation deficient version of the human adenosine 2a receptor for structural studies. Protein Expr. Purif. 49 (2006) 129-137
    • (2006) Protein Expr. Purif. , vol.49 , pp. 129-137
    • Fraser, N.J.1
  • 8
    • 0041876269 scopus 로고    scopus 로고
    • Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification
    • Sarramegna V., Talmont F., Demange P., and Milon A. Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification. Cell. Mol. Life Sci. 60 (2003) 1529-1546
    • (2003) Cell. Mol. Life Sci. , vol.60 , pp. 1529-1546
    • Sarramegna, V.1    Talmont, F.2    Demange, P.3    Milon, A.4
  • 9
    • 33646155331 scopus 로고    scopus 로고
    • Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen
    • André N., Cherouati N., Prual C., Steffan T., Zeder-Lutz G., Magnin T., Pattus F., Michel H., Wagner R., and Reinhart C. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 15 (2006) 1115-1126
    • (2006) Protein Sci. , vol.15 , pp. 1115-1126
    • André, N.1    Cherouati, N.2    Prual, C.3    Steffan, T.4    Zeder-Lutz, G.5    Magnin, T.6    Pattus, F.7    Michel, H.8    Wagner, R.9    Reinhart, C.10
  • 11
    • 0032603760 scopus 로고    scopus 로고
    • Peptide sequencing of 2-DE gel-isolated proteins by nanoelectrospray tandem mass spectrometry
    • Jensen O.M., Wilm M., Shevchenko A., and Mann M. Peptide sequencing of 2-DE gel-isolated proteins by nanoelectrospray tandem mass spectrometry. Methods Mol. Biol. 112 (1999) 571-588
    • (1999) Methods Mol. Biol. , vol.112 , pp. 571-588
    • Jensen, O.M.1    Wilm, M.2    Shevchenko, A.3    Mann, M.4
  • 14
    • 0032521005 scopus 로고    scopus 로고
    • 2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris
    • 2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem. J. 330 (1998) 1137-1147
    • (1998) Biochem. J. , vol.330 , pp. 1137-1147
    • Weiß, H.M.1    Haase, W.2    Michel, H.3    Reiländer, H.4
  • 15
    • 0034635187 scopus 로고    scopus 로고
    • Simple purification of highly active biotinylated P-glycoprotein: enantiomer-specific modulation of drug-stimulated ATPase activity
    • Julien M., Kajiji S., Kaback R.H., and Gros P. Simple purification of highly active biotinylated P-glycoprotein: enantiomer-specific modulation of drug-stimulated ATPase activity. Biochemistry 39 (2000) 75-85
    • (2000) Biochemistry , vol.39 , pp. 75-85
    • Julien, M.1    Kajiji, S.2    Kaback, R.H.3    Gros, P.4
  • 16
    • 6944237210 scopus 로고    scopus 로고
    • The ants go marching two by two: oligomeric structure of G-protein-coupled receptors
    • Javitch J.A. The ants go marching two by two: oligomeric structure of G-protein-coupled receptors. Mol. Pharmacol. 66 (2004) 1077-1082
    • (2004) Mol. Pharmacol. , vol.66 , pp. 1077-1082
    • Javitch, J.A.1
  • 18
    • 1942468187 scopus 로고    scopus 로고
    • Automated large-scale purification of a G protein-coupled receptor for neurotensin
    • White J.F., Trinh L.B., Shiloach J., and Grisshammer R. Automated large-scale purification of a G protein-coupled receptor for neurotensin. FEBS Lett. 564 (2004) 289-293
    • (2004) FEBS Lett. , vol.564 , pp. 289-293
    • White, J.F.1    Trinh, L.B.2    Shiloach, J.3    Grisshammer, R.4
  • 19
    • 33745293916 scopus 로고    scopus 로고
    • Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure
    • Sarramegna V., Muller I., Milon A., and Talmont F. Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure. Cell. Mol. Life Sci. 63 (2006) 1149-1164
    • (2006) Cell. Mol. Life Sci. , vol.63 , pp. 1149-1164
    • Sarramegna, V.1    Muller, I.2    Milon, A.3    Talmont, F.4
  • 20
    • 34347391677 scopus 로고    scopus 로고
    • Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation
    • Klammt C., Srivastava A., Eifler N., Junge F., Beyermann M., Schwarz D., Michel H., Doetsch V., and Bernhard F. Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS J. 274 (2007) 3257-3269
    • (2007) FEBS J. , vol.274 , pp. 3257-3269
    • Klammt, C.1    Srivastava, A.2    Eifler, N.3    Junge, F.4    Beyermann, M.5    Schwarz, D.6    Michel, H.7    Doetsch, V.8    Bernhard, F.9
  • 22
    • 15744391469 scopus 로고    scopus 로고
    • Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors
    • Ishihara G., Goto M., Saeki M., Ito K., Hori T., Kigawa T., Shirouzu M., and Yokoyama S. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. Protein Expr. Purif. 41 (2005) 27-37
    • (2005) Protein Expr. Purif. , vol.41 , pp. 27-37
    • Ishihara, G.1    Goto, M.2    Saeki, M.3    Ito, K.4    Hori, T.5    Kigawa, T.6    Shirouzu, M.7    Yokoyama, S.8
  • 23
    • 33747787335 scopus 로고    scopus 로고
    • Bacterial expression of functional, biotinylated peripheral cannabinoid receptor CB2
    • Krepkiy D., Wong K., Gawrisch K., and Yeliseev A. Bacterial expression of functional, biotinylated peripheral cannabinoid receptor CB2. Protein Expr. Purif. 49 (2006) 60-70
    • (2006) Protein Expr. Purif. , vol.49 , pp. 60-70
    • Krepkiy, D.1    Wong, K.2    Gawrisch, K.3    Yeliseev, A.4
  • 24
    • 33744491058 scopus 로고    scopus 로고
    • Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain
    • Jidenko M., Lenoir G., Fuentes J.M., le Maire M., and Jaxel C. Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain. Protein Expr. Purif. 48 (2006) 32-42
    • (2006) Protein Expr. Purif. , vol.48 , pp. 32-42
    • Jidenko, M.1    Lenoir, G.2    Fuentes, J.M.3    le Maire, M.4    Jaxel, C.5
  • 25
    • 0013091910 scopus 로고    scopus 로고
    • Inhibition of tobacco etch virus protease activity by detergents
    • Mohanty A.K., Simmons C.R., and Wiener M.C. Inhibition of tobacco etch virus protease activity by detergents. Protein Expr. Purif. 27 (2003) 109-114
    • (2003) Protein Expr. Purif. , vol.27 , pp. 109-114
    • Mohanty, A.K.1    Simmons, C.R.2    Wiener, M.C.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.