Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain

Protein Expression and Purification

Volumn 48, Issue 1, 2006, Pages 32-42

  Jidenko, Marie ^a   Lenoir, Guillaume ^a   Fuentes, José M ^a   Maire, Marc le ^a   Jaxel, Christine ^a  

^a CNRS   (France)

Author keywords

Affinity purification; Biotinylated fusion protein; Expression in yeast; Membrane protein

Indexed keywords

AVIDIN; HYBRID PROTEIN; MEMBRANE PROTEIN; SARCOPLASMIC RETICULUM CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; THROMBIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BINDING SITE; BIOTINYLATION; GEL CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN TERTIARY STRUCTURE; RABBIT; SACCHAROMYCES CEREVISIAE; SARCOPLASMIC RETICULUM; SOLUBILITY; SPECTROFLUOROMETRY;

ANIMALS; AVIDIN; BINDING SITES; BIOTINYLATION; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; MEMBRANE PROTEINS; PROTEIN STRUCTURE, TERTIARY; RABBITS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SARCOPLASMIC RETICULUM; SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; THROMBIN;

MAMMALIA; ORYCTOLAGUS CUNICULUS; SACCHAROMYCES CEREVISIAE;

EID: 33744491058     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.03.001     Document Type: Article

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