Differential effects of diazepam treatment and withdrawal on recombinant GABAA receptor expression and functional coupling

Brain Research

Volumn 1246, Issue , 2008, Pages 29-40

  Švob Štrac, Dubravka ^a   Vlainić, Josipa ^a   Jazvinšćak Jembrek, Maja ^a   Peričić, Danka ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

Diazepam treatment; HEK 293 cell; Ligand binding; mRNA and protein expression; Recombinant GABAA receptor; Withdrawal

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; 4 AMINOBUTYRIC ACID A RECEPTOR ALPHA1; 4 AMINOBUTYRIC ACID A RECEPTOR BETA2; 4 AMINOBUTYRIC ACID A RECEPTOR GAMMA2; DIAZEPAM; FLUNITRAZEPAM; FLUNITRAZEPAM H 3; MESSENGER RNA; MUSCIMOL; MUSCIMOL H 3; TERT BUTYLBICYCLOORTHOBENZOATE; TERT BUTYLBICYCLOORTHOBENZOATE H 3; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; BINDING SITE; CELL COUNT; CELL GROWTH; CELL MEMBRANE; CELL PROLIFERATION; CELL STRAIN HEK293; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG BINDING; DRUG EFFECT; DRUG TOLERABILITY; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LONG TERM EXPOSURE; MEMBRANE BINDING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RECEPTOR AFFINITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSLATION; UPREGULATION; WESTERN BLOTTING;

BICYCLO COMPOUNDS, HETEROCYCLIC; BINDING SITES; CELL COUNT; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; DIAZEPAM; FLUNITRAZEPAM; GABA AGONISTS; GABA MODULATORS; GENE EXPRESSION; HUMANS; MUSCIMOL; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RECEPTORS, GABA-A; TRANSCRIPTIONAL ACTIVATION;

EID: 57249095716     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2008.09.093     Document Type: Article

References (67)

1. A receptors fail to show anticonvulsant cross tolerance to diazepam or zolpidem. Neuropharmacology 55 (2008) 148-153
2. A receptors uncouples allosteric binding: studies on possible mechanisms. J. Neurochem. 79 (2001) 1100-1108
3. Barnard E.A., Skolnick P., Olsen R.W., Möhler H., Sieghart W., Biggio G., Braestrup C., Bateson A.N., and Langer Z. International union of pharmacology: XV. Subtypes of γ-aminobutyric acidA receptors: classification on the basis of subunit structure and receptor function. Pharmacol. Rev. 50 (1998) 291-313
4. Bateson A.N. Basic pharmacological mechanisms involved in benzodiazepine tolerance and withdrawal. Curr. Pharm. Des. 8 (2002) 5-21
5. A receptors detected with a novel technique. J. Neurochem. 92 (2005) 859-866
6. A receptors. J. Recept. Signal. Transduct. Res. 17 (1997) 99-113
7. A receptor subunits. Mol. Pharmacol. 53 (1998) 295-303
8. A receptor function and cell surface expression. Pharmacol. Ther. 94 (2002) 113-122
9. 1 subunit protein in rat cerebellar granule cells. Br. J. Pharm. 118 (1996) 1103-1110
10. Busto U.E., Sykora K., and Sellers E.M. A clinical scale to assess benzodiazepine withdrawal. J. Clin. Psychopharmacol. 9 (1989) 412-416
11. A receptor subunit mRNAs in rat cerebral cortex: competitive, quantitative reverse transcriptase-polymerase chain reaction analysis. Mol. Pharmacol. 48 (1995) 861-868
12. 4 subunit gene induced by withdrawal of, but not by long-term treatment with, benzodiazepine full or partial agonists. Mol. Brain Res. 92 (2001) 138-148
13. Fuchs K., and Sieghart W. Evidence for the existence of several different α- and β-subunits of the GABA/benzodiazepine receptor complex from rat brain. Neurosci. Lett. 97 (1989) 329-333
14. 3H]flunitrazepam binding in human embryonic kidney cell. Eur. J. Pharmacol. Mol. Pharmacol. Section 289 (1995) 87-95
15. Gallager D.W., Lakoski J.M., Gonsalves S.F., and Rauch S.L. Chronic benzodiazepine treatment decreases postsynaptic GABA sensitivity. Nature 308 (1984) 74-77
16. Garattini S., Mussini E., Marcucci F., and Guaitani A. Metabolic studies on benzodiazepines in various animal species. In: Garattini S., Mussini E., and Randall L.O. (Eds). Benzodiazepines (1973), Raven Press, New York 75-98
17. Gonsalves S.F., and Gallager D.W. Time course for development of anticonvulsant tolerance and GABAergic subsensitivity after chronic diazepam. Brain Res. 405 (1987) 94-99
18. Gou D.M., Chow L.M., Chen N.Q., Jiang D.H., and Li W.X. Construction and characterization of a cDNA library from 4-week-old human embryo. Gene 278 (2001) 141-147
19. Haefely W., Kulcsár A., Möhler H., Pieri L., Polc P., and Schaffner R. Possible involvement of GABA in the central actions of benzodiazepines. Adv. Biochem. Psychopharmacol. 14 (1975) 131-151
20. A receptor. J. Neurosci. 28 (2008) 3490-3499
21. A subunit mRNA in rat brain. J. Mol. Neurosci. 2 (1990) 101-107
22. Hollister L.E., Conley F.K., Britt R.H., and Shuer L. Long-term use of diazepam. J. Am. Med. Assoc. 246 (1981) 1568-1570
23. A receptor subunit mRNAs in the rat cortex. Neuropharmacology 35 (1996) 1457-1463
24. 2-subunit gene. Mol. Brain Res. 48 (1997) 164-166
25. Hu X.-J., and Ticku M.K. Chronic benzodiazepine agonist treatment produces functional uncoupling of the γ-aminobutyric acid-benzodiazepine receptor ionophore complex in cortical neurons. Mol. Pharmacol. 45 (1994) 618-625
26. A receptor subunit expression in rat neocortex during tolerance to diazepam. Mol. Pharm. 49 (1996) 822-831
27. A receptor trafficking and its role in the dynamic modulation of neuronal inhibition. Nat. Rev. Neurosci. 9 (2008) 331-343
28. Jatlow P., Dobular K., and Bailey D. Serum diazepam concentrations in overdose. Am. J. Clin. Pathol. 72 (1979) 571-577
29. Jones A.W., Holmgren A., and Holgren P. High concentrations of diazepam and nordiazepam in blood of impaired drivers: association with age, gender and spectrum of other drugs present. Forensic Sci. Int. 146 (2004) 1-7
30. 1 subunit gene. J. Neurochem. 62 (1994) 1643-1646
31. A receptors expressed in stably transfected cells. J. Neurochem. 63 (1994) 2349-2352
32. A receptors. Prog. Neurobiol. 67 (2002) 113-159
33. - flux. Pharmacol. Biochem. Behav. 33 (1989) 465-468
34. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
35. A receptor subtypes really occur in the brain. Trends Neurosci. 19 (1996) 139-143
36. A receptor complex binding and function. J. Pharmacol. Exp. Ther. 246 (1988) 177-182
37. Nusser Z., Hajos N., Somogyi P., and Mody I. Increased number of synaptic GABA(A) receptors underlies potentiation at hippocampal inhibitory synapses. Nature 395 (1998) 172-177
38. O'Brien C.P. Benzodiazepine use, abuse and dependence. J. Clin. Psychiat. 66 (2005) 22-33
39. 3H]muscimol binding in the human brain post mortem. Eur. J. Pharmacol. 482 (1993) 117-125
40. A receptors. Eur. J. Pharmacol. 360 (1998) 99-104
41. A receptors stably expressed in HEK 293 cells. Biomed. Pharmacother. 55 (2001) 221-228
42. A receptors. Eur. J. Pharmacol. 482 (2003) 117-125
43. Peričić D., Jazvinšćak Jembrek M., Švob Štrac D., Lazić J., and Rajčan Špoljarić I. Enhancement of benzodiazepine binding sites following chronic treatment with flumazenil. Eur. J. Pharmacol. 507 (2005) 7-13
44. A receptors. Naunyn-Schmiedeberg's Arch. Pharmacol. 375 (2007) 177-187
45. A receptor subunit expression in rat neocortical areas. Neuroscience 79 (1997) 477-487
46. A receptors expressed in Sf9 cells: ligand efficacy and subtype selectivity. J. Pharmacol. Exp. Ther. 276 (1996) 882-890
47. Robertson J.R., and Treasure W. Benzodiazepine abuse. Nature and extent of the problem. CNS Drugs 5 (1996) 137-146
48. A receptor regulation in culture: altered allosteric interactions following prolonged exposure to benzodiazepines, barbiturates, and methylxanthines. Mol. Pharmacol. 37 (1990) 710-719
49. Rosenberg H.C., and Chiu T.H. Time course for development of benzodiazepine tolerance and physical dependence. Neurosci. Biobehav. Rev. 9 (1985) 123-131
50. A receptor subtypes mediate the anxiolytic, abuse-related, and motor effects of benzodiazepine-like drugs in primates. Proc. Natl. Acad. Sci. U.S.A. 102 (2005) 915-920
51. A receptor subtypes: dissecting their pharmacological functions. Trends Pharmacol. Sci. 22 (2001) 188-194
52. Seivewright N., and Dougal W. Withdrawal symptoms from high dose benzodiazepines in poly drug users. Drug Alcohol Depend. 32 (1993) 15-23
53. Shaw G., Morse S., Ararat M., and Graham F.L. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16 (2002) 869-871
54. Stephens D.N., and Schneider H.H. Tolerance to the benzodiazepine diazepam in an animal model of anxiolytic activity. Psychopharmacology 87 (1985) 322-327
55. Suzuki T., Higgins P.J., and Crawford D.R. Control selection for RNA quantitation. BioTechniques 29 (2000) 332-337
56. Thomas P., and Smart T.G. HEK293 cell line: a vehicle for the expression of recombinant proteins. J. Pharmacol. Toxicol. Meth. 51 (2005) 187-200
57. Tietz E.I., Rosenberg H.C., and Chiu T.H. Autoradiographic localization of benzodiazepine receptor down-regulation. J. Pharmacol. Exp. Ther. 236 (1986) 284-292
58. Tietz E.I., Costa T.H., and Rosenberg H.C. Regional GABA/benzodiazepine receptor/chloride channel opening after acute and chronic benzodiazepine treatment. Eur. J. Pharmacol. 167 (1989) 57-65
59. A receptor function. Life Sci. 59 (1996) 1631-1641
60. A receptors for the development of tolerance to the sedative action of diazepam in mice. J. Neurosci. 24 (2004) 6785-6790
61. A receptor subtypes: any clues to the mechanism of benzodiazepine dependence?. Curr. Opin. Pharmacol. 5 (2005) 47-52
62. A receptors containing the alpha 4 subunit. Mol. Pharmacol. 50 (1996) 670-678
63. A receptors via two distinct and separable mechanisms. Nat. Neurosci. 3 (2000) 1274-1281
64. A receptors. Neuropharmacology 41 (2001) 539-545
65. A receptor alpha 4 subunit. FEBS Lett. 289 (1991) 227-230
66. Wong G., Lyon T., and Skolnick P. Chronic exposure to benzodiazepine receptor ligands uncouples the γ-aminobutyric acid type A receptor in WSS-1 cells. Mol. Pharmacol. 46 (1994) 1056-1062
67. A receptor subunit mRNAs during chronic treatment of rats with diazepam. J. Mol. Neurosci. 5 (1994) 105-120