Increasing yeast secretion of heterologous proteins by regulating expression rates and post-secretory loss

Biotechnology and Bioengineering

Volumn 101, Issue 6, 2008, Pages 1264-1275

  Huang, Dagang ^a   Gore, Patrick R ^a   Shusta, Eric V ^a  

^a University of Wisconsin Madison   (United States)

Author keywords

Heterologous protein secretion; Saccharomyces cerevisiae; Yeast

Indexed keywords

ACIDS; AGGLOMERATION; OPTIMIZATION; YEAST;

ADDITIVE EFFECTS; CASAMINO ACIDS; CELLULAR RESPONSES; COUPLED PROCESSES; DIRECT OPTIMIZATIONS; HETEROLOGOUS PROTEIN SECRETION; HETEROLOGOUS PROTEINS; INDUCTION TEMPERATURES; INTRACELLULAR PROTEINS; NUTRIENT DEPLETIONS; PROTEIN EXPRESSIONS; PROTEIN LOSSES; PROTEIN SYNTHESES; REFEEDING; SACCHAROMYCES CEREVISIAE; SECRETED PROTEINS; SECRETION SYSTEMS; SECRETORY PATHWAYS; UNFOLDED PROTEIN RESPONSES; YEAST CELLS; YEAST NITROGEN BASE;

PROTEIN FOLDING;

CASAMINO ACID; NITROGEN; PROTEIN;

ARTICLE; FUNGAL METABOLISM; FUNGUS GROWTH; HUMAN; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN INDUCTION; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; TEMPERATURE DEPENDENCE;

AMINO ACIDS; CULTURE MEDIA; CYTOPLASM; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; NITROGEN; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE;

SACCHAROMYCES CEREVISIAE;

EID: 56749112212     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22019     Document Type: Article

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