메뉴 건너뛰기




Volumn 22, Issue 5, 2006, Pages 1249-1255

Optimization of the human adenosine A2a receptor yields in Saccharomyces cerevisiae

Author keywords

[No Author keywords available]

Indexed keywords

CELL DENSITY; G-PROTEIN COUPLED RECEPTORS (GPCR); SACCHAROMYCES CEREVISIAE;

EID: 33749987003     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp050431r     Document Type: Article
Times cited : (30)

References (28)
  • 1
    • 12344250438 scopus 로고    scopus 로고
    • Structural genomics of GPCRs
    • Lundstrom, K. Structural genomics of GPCRs. Trends Biotechnol. 2005, 23(2), 103-108.
    • (2005) Trends Biotechnol. , vol.23 , Issue.2 , pp. 103-108
    • Lundstrom, K.1
  • 2
    • 0037020329 scopus 로고    scopus 로고
    • Drug design strategies for targeting G-protein-coupled receptors
    • Kablunde, T.; Hessler, G. Drug design strategies for targeting G-protein-coupled receptors. ChemBioChem 2002, 3, 928-944.
    • (2002) ChemBioChem , vol.3 , pp. 928-944
    • Kablunde, T.1    Hessler, G.2
  • 3
    • 0041876269 scopus 로고    scopus 로고
    • Heterologous expression of G protein-coupled receptors: Comparison of expression systems from the standpoint of large-scale production and purification
    • Sarramegna, V.; Talmont, F.; Demange, P.; Milon, A. Heterologous expression of G protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification. Cell. Mol. Life Sci. 2003, 60, 1529-1546.
    • (2003) Cell. Mol. Life Sci. , vol.60 , pp. 1529-1546
    • Sarramegna, V.1    Talmont, F.2    Demange, P.3    Milon, A.4
  • 4
    • 12244292640 scopus 로고    scopus 로고
    • Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli
    • Weiss, H. M.; Grisshammer, R. Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli. Eur. J. Biochem. 2002, 269 (1), 82-92.
    • (2002) Eur. J. Biochem. , vol.269 , Issue.1 , pp. 82-92
    • Weiss, H.M.1    Grisshammer, R.2
  • 5
    • 4344579363 scopus 로고    scopus 로고
    • A pedestrian guide to membrane protein crystallization
    • Wiener, M. C. A pedestrian guide to membrane protein crystallization. Methods 2004, 34, 364-372.
    • (2004) Methods , vol.34 , pp. 364-372
    • Wiener, M.C.1
  • 6
    • 0037450520 scopus 로고    scopus 로고
    • Preface: Overexpression of integral membrane proteins
    • Grisshammer, R.; Tate, C. Preface: overexpression of integral membrane proteins. Biochim. Biophis. Acta 2003, 1610 (1), 1.
    • (2003) Biochim. Biophis. Acta , vol.1610 , Issue.1 , pp. 1
    • Grisshammer, R.1    Tate, C.2
  • 7
    • 0034604351 scopus 로고    scopus 로고
    • Structure-rhodopsin sees the light
    • Bourne, H. R.; Meng, E. C. Structure-rhodopsin sees the light. Science 2000, 289, 733-734.
    • (2000) Science , vol.289 , pp. 733-734
    • Bourne, H.R.1    Meng, E.C.2
  • 9
    • 0031833150 scopus 로고    scopus 로고
    • Adenosine receptors: New opportunities for future drugs
    • Poulson, S. A.; Quinn, R. J. Adenosine receptors: new opportunities for future drugs. Bioorg. Med. Chem. 1998, 6, 619-641.
    • (1998) Bioorg. Med. Chem. , vol.6 , pp. 619-641
    • Poulson, S.A.1    Quinn, R.J.2
  • 10
    • 0034846624 scopus 로고    scopus 로고
    • Adenosine, adenosine receptors and myocardial protection: An updated overview
    • Mubagwa, K.; Flameng, W. Adenosine, adenosine receptors and myocardial protection: an updated overview. Cardiovasc. Res. 2001, 52, 25-39.
    • (2001) Cardiovasc. Res. , vol.52 , pp. 25-39
    • Mubagwa, K.1    Flameng, W.2
  • 11
    • 3543141563 scopus 로고    scopus 로고
    • Saccharomyces cerevisiae protein expression: From protein production to protein engineering
    • Horizon Scientific Press: Norwich, U.K.
    • Niebauer, R. T.; Robinson, A. S. Saccharomyces cerevisiae protein expression: From protein production to protein engineering. In Expression Technologies, Horizon Scientific Press: Norwich, U.K., 2004.
    • (2004) Expression Technologies
    • Niebauer, R.T.1    Robinson, A.S.2
  • 12
    • 0031663369 scopus 로고    scopus 로고
    • The green fluorescent protein
    • Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 1998, 67, 509-544.
    • (1998) Annu. Rev. Biochem. , vol.67 , pp. 509-544
    • Tsien, R.Y.1
  • 14
    • 0141533196 scopus 로고    scopus 로고
    • Coexpression of molecular chaperones does not improve the heterologous expression of mammalian G protein-coupled receptor expression in yeast
    • Butz, J.; Niebauer, R. T.; Robinson, A. S. Coexpression of molecular chaperones does not improve the heterologous expression of mammalian G protein-coupled receptor expression in yeast. Biotechnol. Bioeng. 2003, 84 (3), 292-304.
    • (2003) Biotechnol. Bioeng. , vol.84 , Issue.3 , pp. 292-304
    • Butz, J.1    Niebauer, R.T.2    Robinson, A.S.3
  • 15
    • 3543088051 scopus 로고    scopus 로고
    • Decreases in yeast expression yields of the human adenosine A2a receptor are a result of translational or post-translational events
    • Niebauer, R. T.; Wedekind, A.; Robinson, A. S. Decreases in yeast expression yields of the human adenosine A2a receptor are a result of translational or post-translational events. Protein Expression Purif. 2004, 57, 134-143.
    • (2004) Protein Expression Purif. , vol.57 , pp. 134-143
    • Niebauer, R.T.1    Wedekind, A.2    Robinson, A.S.3
  • 16
    • 33645415883 scopus 로고    scopus 로고
    • 2005 Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae
    • Niebauer, R. T.; Robinson, A. S. 2005 Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae. Protein Expression Purif. 2005, 46 (2), 204-211.
    • (2005) Protein Expression Purif. , vol.46 , Issue.2 , pp. 204-211
    • Niebauer, R.T.1    Robinson, A.S.2
  • 17
    • 33646155331 scopus 로고    scopus 로고
    • Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen
    • André, N.; Cherouati, N.; Prual, C.; Steffan, T.; Zeder-Lutz, G.; Magnin, T.; Pattus, F.; Michel, H.; Wagner, R.; Reinhart, C. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 2000, 15, 1115-1126.
    • (2000) Protein Sci. , vol.15 , pp. 1115-1126
    • André, N.1    Cherouati, N.2    Prual, C.3    Steffan, T.4    Zeder-Lutz, G.5    Magnin, T.6    Pattus, F.7    Michel, H.8    Wagner, R.9    Reinhart, C.10
  • 18
    • 0031104672 scopus 로고    scopus 로고
    • Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae
    • Parekh, R. N.; Wittrup, K. D. Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae. Biotechnol. Prog. 1997, 13 (2), 117-122.
    • (1997) Biotechnol. Prog. , vol.13 , Issue.2 , pp. 117-122
    • Parekh, R.N.1    Wittrup, K.D.2
  • 19
    • 0031879861 scopus 로고    scopus 로고
    • 1998 Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments
    • Shusta, E.; Raines, R. T.; Pluckthun, A.; Wittrup, K. D. 1998 Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat. Biotechnol. 1998, 16, 773-777.
    • (1998) Nat. Biotechnol. , vol.16 , pp. 773-777
    • Shusta, E.1    Raines, R.T.2    Pluckthun, A.3    Wittrup, K.D.4
  • 20
    • 0036514182 scopus 로고    scopus 로고
    • Optimizing functional versus total expression of the human μ-opioid receptor in Pichia pastoris
    • Sarramegna, V.; Demage, P.; Milon, A.; Talmont, F. Optimizing functional versus total expression of the human μ-opioid receptor in Pichia pastoris. Protein Expression Purif. 2002, 24, 212-220.
    • (2002) Protein Expression Purif. , vol.24 , pp. 212-220
    • Sarramegna, V.1    Demage, P.2    Milon, A.3    Talmont, F.4
  • 24
    • 4243638669 scopus 로고    scopus 로고
    • Clontech Laboratories, Palo Alto, CA
    • Living Color User Manual; Clontech Laboratories, Palo Alto, CA, 1999.
    • (1999) Living Color User Manual
  • 26
    • 0025168284 scopus 로고
    • Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: Bovine lysozyme
    • Brierley, R. A.; Bussineau, C.; Kosson, R.; Melton, A.; Siegel, R. S. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann. N.Y. Acad. Sci. 1990, 589, 350-362.
    • (1990) Ann. N.Y. Acad. Sci. , vol.589 , pp. 350-362
    • Brierley, R.A.1    Bussineau, C.2    Kosson, R.3    Melton, A.4    Siegel, R.S.5
  • 27
    • 0001078966 scopus 로고
    • Production of foreign proteins in the yeast Pichia pastoris
    • Cregg, J. M.; Higgins, D. R. Production of foreign proteins in the yeast Pichia pastoris. Can. J. Bot. 1995, 73, 91-97.
    • (1995) Can. J. Bot. , vol.73 , pp. 91-97
    • Cregg, J.M.1    Higgins, D.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.