Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast

Biotechnology and Bioengineering

Volumn 84, Issue 3, 2003, Pages 292-304

  Butz, James A ^a   Niebauer, Ronald T ^a   Robinson, Anne Skaja ^a  

^a University of Delaware   (United States)

Author keywords

BiP; Calnexin; Folding bottleneck; GPCR; Heterologous expression; Neurokinin 1; NK 1; PDI

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; CHEMICAL BONDS; COBALT; PLASMAS; PROTEINS; YEAST;

RECEPTOR EXPRESSION;

BIOTECHNOLOGY;

ADENOSINE A2 RECEPTOR; CALNEXIN; CHAPERONE; G PROTEIN COUPLED RECEPTOR; PROTEIN DISULFIDE ISOMERASE; SUBSTANCE P RECEPTOR;

ARTICLE; CELL MEMBRANE; CELL SURFACE; DISULFIDE BOND; GLYCOSYLATION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; YEAST;

ANIMALS; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; MAMMALS; MICE; MOLECULAR CHAPERONES; PROTEIN ENGINEERING; RATS; RECEPTOR, ADENOSINE A2A; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, NEUROKININ-1; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

MAMMALIA;

EID: 0141533196     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10771     Document Type: Article

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