Hec1 overexpression hyperactivates the mitotic checkpoint and induces tumor formation in vivo

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 43, 2008, Pages 16719-16724

  Diaz Rodríguez, Elena ^a,b   Sotillo, Rocio ^a   Schvartzman, Juan Manuel ^a   Benezra, Robert ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b CSIC   (Spain)

Author keywords

Aneuploidy; Cancer; Chromosome instability

Indexed keywords

BUB3 PROTEIN; DOXYCYCLINE; HIGHLY EXPRESSED IN CANCER 1 PROTEIN; MEMBRANE PROTEIN; PROTEIN MAD2; UNCLASSIFIED DRUG;

ANEUPLOIDY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER GRADING; CARCINOGENESIS; CHROMOSOMAL INSTABILITY; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVATION; FEMALE; FIBROBLAST; GENE OVEREXPRESSION; GENOTYPE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VIVO STUDY; INTESTINE TUMOR; LIVER CELL CARCINOMA; LUNG ADENOMA; LYMPHOCYTE; LYMPHOMA; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROGNOSIS; PROSTATE TUMOR; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SARCOMA; SOUTHERN BLOTTING; TRANSGENIC MOUSE;

ANEUPLOIDY; ANIMALS; CELL CYCLE PROTEINS; CHROMOSOMAL INSTABILITY; DOXYCYCLINE; GENE EXPRESSION REGULATION; KINETOCHORES; MICE; MICE, TRANSGENIC; MITOSIS; NEOPLASMS; NUCLEAR PROTEINS; TISSUE DISTRIBUTION;

EID: 55949096605     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0803504105     Document Type: Article

References (35)

1. Bharadwaj R, Yu H (2004) The spindle checkpoint, aneuploidy, and cancer. Oncogene 23:2016-2027.
2. Wassmann K, Benezra R (2001) Mitotic checkpoints: From yeast to cancer. Curr Opin Genet Dev 11:83-90.
3. Ciferri C, Musacchio A, Petrovic A (2007) The Ndc80 complex: Hub of kinetochore activity. FEBS Lett 581:2862-2869.
4. Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A (2006) The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell 127:983-997.
5. DeLuca JG, et al. (2006) Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127:969-982.
6. Wei RR, Al-Bassam J, Harrison SC (2007) The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment. Nat Struct Mol Biol 14:54-59.
7. Bharadwaj R, Qi W, Yu H (2004) Identification of two novel components of the human NDC80 kinetochore complex. J Biol Chem 279:13076-13085.
8. Ciferri C, et al. (2005) Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. J Biol Chem 280:29088-29095.
9. Hori T, Haraguchi T, Hiraoka Y, Kimura H, Fukagawa T (2003) Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J Cell Sci 116:3347-3362.
10. McCleland ML, et al. (2004) The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. Curr Biol 14:131-137.
11. Musacchio A, Salmon ED (2007) The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol 8:379-393.
12. Martin-Lluesma S, Stucke VM, Nigg EA (2002) Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297:2267-2270.
13. Meraldi P, Draviam VM, Sorger PK (2004) Timing and checkpoints in the regulation of mitotic progression. Dev Cell 7:45-60.
14. Dobles M, Liberal V, Scott ML, Benezra R, Sorger PK (2000) Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2. Cell 101:635-645.
15. Rao CV, et al. (2005) Colonic tumorigenesis in BubR1+/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability. Proc Natl Acad Sci USA 102:4365-4370.
16. Weaver BA, Silk AD, Montagna C, Verdier-Pinard P, Cleveland DW (2007) Aneuploidy acts both oncogenically and as a tumor suppressor. Cancer Cell 11:25-36.
17. Sotillo R, et al. (2007) Mad2 overexpression promotes aneuploidy and tumorigenesis in mice. Cancer Cell 11:9-23.
18. Lehman NL, et al. (2007) Oncogenic regulators and substrates of the anaphase promoting complex/cyclosome are frequently overexpressed in malignant tumors. Am J Pathol 170:1793-1805.
19. Yuan B, et al. (2006) Increased expression of mitotic checkpoint genes in breast cancer cells with chromosomal instability. Clin Cancer Res 12:405-410.
20. Zheng L, Chen Y, Riley DJ, Chen PL, Lee WH (2000) Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p. Mol Cell Biol 20:3529-3537.
21. Chen Y, Riley DJ, Chen PL, Lee WH (1997) HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Mol Cell Biol 17:6049-6056.
22. Hayama S, et al. (2006) Activation of CDCA1-KNTC2, members of centromere protein complex, involved in pulmonary carcinogenesis. Cancer Res 66:10339-10348.
23. Lin YT, Chen Y, Wu G, Lee WH (2006) Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control. Oncogene 25:6901-6914.
24. DeLuca JG, et al. (2003) Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr Biol 13:2103-2109.
25. Furth PA, et al. (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci USA 91:9302-9306.
26. Shepard JL, et al. (2007) A mutation in separase causes genome instability and increased susceptibility to epithelial cancer. Genes Dev 21:55-59.
27. Garber ME, et al. (2001) Diversity of gene expression in adenocarcinoma of the lung. Proc Natl Acad Sci USA 98:13784-13789.
28. Hernando E, et al. (2004) Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature 430:797-802.
29. Tanaka K, et al. (2001) Mitotic checkpoint protein hsMAD2 as a marker predicting liver metastasis of human gastric cancers. Jpn J Cancer Res 92:952-958.
30. van't Veer LJ, et al. (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415:530-536.
31. Michel L, Benezra R, Diaz-Rodriguez E (2004) MAD2-dependent mitotic checkpoint defects in tumorigenesis and tumor cell death: A double-edged sword. Cell Cycle 3:990-992.
32. Michel LS, et al. (2001) MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. Nature 409:355-359.
33. Dai W, et al. (2004) Slippage of mitotic arrest and enhanced tumor development in mice with BubR1 haploinsufficiency. Cancer Res 64:440-445.
34. Harlow E, Lane D (1988) Antibodies: A Laboratory Manual (Cold Spring Harbor Lab Press, New York).
35. Diaz-Rodriguez E, Montero JC, Esparis-Ogando A, Yuste L, Pandiella A (2002) Extracellular signal-regulated kinase phosphorylates tumor necrosis factor α-converting enzyme at threonine 735: A potential role in regulated shedding. Mol Biol Cell 13:2031-2044.