Heating greatly speeds coomassie blue staining and destaining

BioTechniques

Volumn 28, Issue 3, 2000, Pages 426-432

  Wong, Connie ^a   Sridhara, Srinavas ^a   Bardwell, James C A ^a   Jakob, Ursula ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 PROPANOL; ANAZOLENE SODIUM;

ANALYTIC METHOD; ARTICLE; HEATING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; STAINING; ULTRAVIOLET RADIATION;

EID: 0034012213     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283bm07     Document Type: Article

References (8)

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2. Kurien, B.J. and R.H. Scofield. 1998. Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gels. Indian J. Biochem. Biophys. 35:385-389.
3. Meyer, T. S. and B.L. Lambert. 1965. Use of coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips. Biochim. Biophys. Acta 107:144-145.
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5. Sreeramulu, G. and N.K. Singh. 1995. Destaining of Coomassie brilliant blue R-250-stained polyacrylamide gels with sodium chloride solutions. Electrophoresis 16:362-365.
6. Syrovy, I. and Z. Hodny. 1991. Staining and quantification of proteins separated by polyacrylamide gel electrophoresis. J. Chromatog. 569:175-196.
7. Wu, W. and M.J. Welsh. 1995. Rapid Coomassie blue staining and destaining of polyacrylamide gels. BioTechniques 20:386-388.
8. Merril, C.R. 1990. Guide to Protein Purification. Methods Enzymol. 182:477-488.