Choosing a culture medium: making informed choices

Fertility and Sterility

Volumn 90, Issue 3, 2008, Pages 473-483

  Biggers, John D ^a   Summers, Michael C ^b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

amino acids; EDTA; Embryo culture; energy sources; glutamine; one step and two step protocols

Indexed keywords

AMINO ACID; AMMONIA; EDETIC ACID; GLUTAMINE;

BLASTOCYST; EMBRYO CULTURE; FERTILIZATION; HUMAN; INFERTILITY; NONHUMAN; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; QUALITY CONTROL; REVIEW; ZYGOTE;

BLASTOCYST; CULTURE MEDIA; EMBRYO CULTURE TECHNIQUES; HUMANS;

EID: 51449098885     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2008.08.010     Document Type: Review

References (95)

1. Biggers J.D. Fundamentals of the design of culture media that support human preimplantation development. In: Van Blerkom J. (Ed). Essential IVF (2003), Kluwer Academic Press, Norwell, MA 291-332
2. Gardner D.K., and Lane M. Culture and selection of viable human blastocysts: a feasible proposition for human IVF. Hum Reprod Update 3 (1997) 367-382
3. Gardner D.K., and Lane M. Development of viable mammalian embryos in vitro: evolution of sequential media. In: Cibelli J., Lanza R.P., Campbell K.H.S., and West M.D. (Eds). Principles of cloning (2002), Academic Press, New York 187-213
4. Gardner D.K., and Lane M. Towards a single embryo transfer. Reprod BioMed Online 6 (2003) 470-481
5. Pool T.B. An update on embryo culture for human assisted reproductive technology: media, performance, and safety. Semin Reprod Med 23 (2002) 309-318
6. Pool T.B. Recent advances in the production of viable human embryos in vitro. Reprod BioMed Online 4 (2005) 294-302
7. Summers M.C., and Biggers J.D. Chemically defined media and the culture of mammalian preimplantation embryos; historical perspective and current issues. Hum Reprod Update 9 (2003) 557-582
8. Biggers J.D. Thoughts on embryo culture conditions. Reprod BioMed Online 4 Suppl 1 (2002) 30-38
9. AA: is a two-step protocol necessary?. Reprod BioMed Online 5 (2002) 133-140
10. Biggers J.D., McGinnis L.K., and Lawitts J.A. One-step versus two-step culture of mouse reimplantation embryos: is there a difference?. Hum Reprod 20 (2005) 3376-3384
11. Biggers J.D., McGinnis L., and Summers M.C. One step versus two-step culture of mouse preimplantation embryos. Hum Reprod 21 (2006) 1936-1939
12. Whitten W.K. Culture of tubal ova. Nature 177 (1956) 96
13. Whitten W.K. Culture of tubal ova. Nature 179 (1957) 1081-1082
14. Biggers J.D., Whittingham D.G., and Donahue R.P. The pattern of energy metabolism in the mouse oocyte and zygote. Proc Natl Acad Sci USA 58 (1967) 560-567
15. Edwards R.G. Test tube babies. Nature 293 (1981) 253-256
16. Leese H.J., and Barton A.M. Pyruvate and glucose uptake by mouse ova and preimplantation embryos. J Reprod Fertil 72 (1984) 9-13
17. Leese H.J., Conaghan J., Martin K.L., and Hardy K. Early human embryo metabolism. BioEssays 15 (1993) 259-264
18. Hardy K., Hooper M.A.K., Handyside A.H., Rutherford A.J., Winston R.M.L., and Leese H.J. Noninvasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos. Hum Reprod 4 (1989) 188-191
19. Conaghan J., Handyside A.H., Winston R.M.L., and Leese H. Effects of pyruvate and glucose on the development of the human preimplantation embryo. J Reprod Fertil 99 (1993) 87-95
20. Gardner D.K., Lane M., Calderon I., and Leeton J. Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril 65 (1996) 349-353
21. Biggers J.D., and McGinnis L.K. Evidence that glucose is not always an inhibitor of mouse preimplantation development in vitro. Hum Reprod 16 (2001) 153-163
22. Devreker F., and Englert Y. In vitro development and metabolism of the human embryo up to the blastocyst stage. Eur J Obstet Gyn Reprod Biol 92 (2000) 51-56
23. Schini S.A., and Bavister B.D. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol Reprod 39 (1988) 1183-1192
24. Chatot C.L., Ziomek C.A., Bavister B.D., Lewis J.L., and Torres I. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 86 (1989) 679-688
25. Quinn P., Moinipanah R., Steinberg J.M., and Weathersbee P. Successful human in vitro fertilization using a modified human human tubal fluid medium lacking glucose and phosphate ions. Fertil Steril 63 (1995) 922-924
26. Gardner D.K., Pool T.B., and Lane M. Embryo nutrition and energy metabolism and its relationship to embryo growth, differentiation, and viability. Semin Reprod Med 18 (2000) 205-218
27. Dickens C.J., Maguiness S.D., Comer M.T., Palmer A., Rutherford A.J., and Leese H.J. Human tubal fluid: formation and composition during vascular perfusion of the Fallopian tube. Hum Reprod 10 (1995) 505-508
28. Tay J.I., Rutherford A.J., Killick S.R., Maguiness S.D., Partridge R.J., and Leese H.J. Human tubal fluid: production, nutrient composition and response to adrenergic agents. Hum Reprod 12 (1997) 2451-2456
29. Gardner D.K., and Leese H.J. Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro. J Reprod Fertil 88 (1990) 361-368
30. Barak Y., Goldman S., Gonen Y., Nevo Z., Bartoov B., and Kogosowski A. Does glucose affect fertilization, development and pregnancy rates of human in-vitro fertilized oocytes?. Hum Reprod 13 Suppl 4 (1998) 203-211
31. Coates A., Rutheford A.J., Hunter H., and Leese H. Glucose-free medium in human in vitro fertilization and embryo transfer: a large-scale, prospective, randomized clinical trial. Fertil Steril 72 (1999) 229-232
32. Macklon N.S., Pieters M.H.E.C., Hassan M.A., Jeucken P.H.M., Eijkemans M.J.C., and Fauser B.C.J.M. A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development. Hum Reprod 17 (2002) 2700-2705
33. Biggers J.D. Reflections on the culture of the preimplantation embryo. Int J Dev Biol 42 (1988) 879-884
34. Biggers J.D. The culture of the preimplantation mammalian embryo. In: Gianaroli L., Campana A., and Trounson A.O. (Eds). Implantation in mammals (1993), Raven Press, New York 123-136
35. Abramczuk J., Solter D., and Koprowski H. The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev Biol 61 (1977) 378-383
36. Hoshi M., and Toyoda Y. Effect of EDTA on the preimplantation development of mouse embryos fertilized in vitro. Jpn J Zootech Sci 56 (1985) 931-937
37. Loutradis D., John D., and Keissling A.A. Hypoxanthine causes a 2-cell block in random-bred mouse embryos. Biol Reprod 37 (1987) 311-316
38. Fissore R.A., Jackson K.V., and Kiessling A.A. Mouse zygote development in culture medium without protein in the presence of ethylenediamine-tetraacetic acid. Biol Reprod 41 (1989) 835-841
39. Nasr-Esfahani N.H., Winston N.J., and Johnson M.H. Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. J Reprod Fertil 96 (1992) 219-231
40. Gardner D.K., and Lane M. Alleviation of the "2-cell block" and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod 11 (1996) 2703-2712
41. Gardner D.K., Lane M.W., and Lane M. EDTA stimulates cleavage stage bovine embryo development in culture but inhibits blastocyst development and differentiation. Mol Reprod Dev 57 (2000) 256-261
42. Lawitts J.A., and Biggers J.D. Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil 91 (1991) 543-556
43. Gardner D.K., and Lane M. Amino acids and ammonium production regulate mouse embryo development in culture. Biol Reprod 48 (1993) 377-385
44. Lane M., and Gardner D.K. Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J Reprod Fertil 102 (1994) 305-312
45. Biggers J.D., McGinnis L.K., and Summers M.C. Discrepancies between the effects of glutamine in cultures of preimplantation mouse embryos. Reprod BioMed Online 9 (2004) 70-73
46. Summers M.C., McGinnis L.K., Lawitts J.A., and Biggers J.D. Mouse embryo development following IVF in media containing either L-glutamine or glycyl-L-glutamine. Hum Reprod 20 (2005) 1364-1371
47. Daniel J.C. Studies on the growth of 5-day old rabbit blastocysts in vitro. J Embryol Exp Morph 13 (1965) 83-96
48. Naglee D.L., Maurer R.R., and Foote R.H. Effect of osmolarity on the in vitro development of rabbit embryos in a chemically defined medium. Exp Cell Res 58 (1969) 331-333
49. Kane M.T., and Foote R.H. Culture of 2- and 4-cell rabbit embryos to the expanding blastocyst stage in synthetic medium. Proc Soc Exp Biol Med 133 (1970) 921-925
50. Menezo Y.J.R. Milieu synthetique pour la survie, et maturation des gametes et pour la culture de l'oeuf feconde. C R Acad Sci (Paris) 282 (1976) D1967-D1970
51. Menezo Y. Serum is not necessary in human in vitro fertilization, early embryo culture and transfer. Fertil Steril 42 (1984) 750-755
52. Carney E., and Bavister B.D. Stimulatory and inhibitory effects of amino acids on the development of hamster eight-cell embryos in vitro. J In Vitro Fertil Embryo Transfer 4 (1987) 162-167
53. Lawitts J.A., and Biggers J.D. Culture of preimplantation embryos. Methods Enzymol 225 (1993) 153-164
54. Gardner D.K., Rodriegez-Martinez H., and Lane M. Fetal development after transfer is increased by replacing protein with glycosoaminoglycan hyaluronan for mouse embryo culture and transfer. Hum Reprod 14 (1999) 2575-2580
55. Lane M., and Gardner D.K. Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression, and subsequently alters fetal development in the mouse. Biol Reprod 69 (2003) 1109-1117
56. Nakazawa T., Ohashi K., Yamada M., Shinoda S., Saji F., Murata Y., and Araki H. Effect of different concentrations of amino acids in human serum and follicular fluid on the development of one-cell mouse embryos in vitro. J Reprod Fertil 111 (1997) 327-332
57. Whitten W.K., and Biggers J.D. Complete development in vitro of the preimplantation stages of the mouse in a simple chemically defined medium. J Reprod Fertil 17 (1968) 399-401
58. Brinster R.L. Studies on the development of mouse embryos in vitro. III. The effects of fixed-nitrogen source. J Exp Zool 158 (1965) 69-78
59. Mehta T.S., and Kiessling A.A. Development potential of mouse embryos conceived in vitro and cultured in ethylenediaminetetraacetic acid with or without amino acids or serum. Biol Reprod 43 (1990) 600-606
60. Caro C.M., and Trounson A. The effect of protein on preimplantation mouse embryo development in vitro. J In Vitro Embryo Transfer 1 (1984) 183-187
61. Caro C.M., and Trounson A. Successful fertilization, embryo development, and pregnancy in human in vitro fertilization (IVF) using a chemically defined culture medium containing no protein. J In Vitro Embryo Transfer 3 (1986) 215-217
62. Bavister B.D. Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update 1 (1995) 91-148
63. Gardner D.K. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biol Int 18 (1994) 1163-1179
64. Biggers J.D. Pioneering mammalian embryo culture. In: Bavister B.D. (Ed). The mammalian preimplantation embryo (1987), Plenum Publishing, New York 1-22
65. Ham R.G. An improved nutrient solution for diploid Chinese hamster cells in a synthetic medium supplemented with purified protein fractions. Exp Cell Res 29 (1963) 515-526
66. Ho Y., Wigglesworth K., Eppig J.J., and Schultz R. Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Mol Reprod Dev 38 (1995) 131-141
67. Eagle H. Amino acid metabolism in mammalian cell cultures. Science 130 (1959) 432-437
68. Cholewa J.A., and Whitten W.K. Devlopment of two-cell mouse embryos in the absence of a fixed nitrogen source. J Reprod Fertil 22 (1970) 553-555
69. Biggers J.D., Summers M.C., and McGinnis L.K. Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in mouse preimplantation embryo culture media. Hum Reprod Update 3 (1997) 125-135
70. Biggers J.D., McGinnis L.K., and Raffin M. Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol Reprod 63 (2000) 281-293
71. Walker A.K., Hill J.L., Kleeman D.O., and Nancarrow C.D. Development of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentrations. Biol Reprod 55 (1996) 701-708
72. Houghton F.D., Hawkhead J.A., Humpherson P.G., Hogg J.E., Balen A.H., Rutherford A.J., and Leese H.J. Non-invasive amino acid turnover predicts human embryo developmental capacity. Hum Reprod 17 (2002) 999-1005
73. Brison D.R., Houghton F.D., Falconer D., Roberts S.A., Hawkhead J., Humpherson P.G., et al. Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover. Hum Reprod 19 (2004) 2319-2324
74. Stokes P.J., Hawkhead J.A., Fawthrop R.K., Picton H.M., Sharma V., Leese H.L., et al. Metabolism of human embryos following cryopreservation: Implications for the safety and selection of embryos for transfer in clinical IVF. Hum Reprod 22 (2007) 829-835
75. Lane M., and Gardner D.K. Differential regulation of mouse embryo development and viability by amino acids. J Reprod Fertil 109 (1997) 153-164
76. Lane M., Hooper K., and Gardner D.K. Effect of essential amino acids on mouse embryo viability and ammonium production. J Assist Reprod Genet 18 (2001) 519-525
77. Jozwik M., Teng C., and Battaglia F.C. Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Hum Reprod 21 (2006) 2776-2782
78. Lamb V.K., and Leese H.J. Uptake of a mixture of amino acids by mouse blastocysts. J Reprod Fertil 102 (1994) 169-175
79. Orsi N.M., and Leese H.J. Ammonium exposure and pyruvate affect the amino acid metabolism of bovine blastocysts in vitro. Reproduction 127 (2004) 131-140
80. Booth P.J., Humpherson P.G., Watson T.J., and Leese H.J. Amino acid depletion and appearance during porcine preimplantation embryo development in vitro. Reproduction 130 (2005) 655-668
81. Booth P.J., Watson T.J., and Leese H.J. Prediction of porcine blastocyst formation using morphological, kinetic, and amino acid depletion and appearance criteria determined during the early cleavage of in vitro-produced embryos. Biol Reprod 77 (2007) 765-779
82. Leese H.J. Quiet please, do not disturb: a hypothesis of embryo metabolism and viability. BioEssays 24 (2002) 845-849
83. Leese H.J. What does an embryo need?. Hum Fertil 6 (2003) 180-185
84. Leese H.J., Sturmey R.G., Baumann C.G., and McEvoy T.G. Embryo viability and metabolism: obeying the quiet rules. Hum Reprod 22 (2007) 3047-3050
85. Biggers J.D., Rinaldini L.M., and Webb M. The study of growth factors in tissue culture. Soc Exp Biol 11 (1957) 264-297
86. Barnes F.L., Crombie A., Gardner D.K., Kausche A., Lacham-Kaplan O., Suikkari A.-M., et al. Blastocyst development and birth after in vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 10 (1995) 3243-3247
87. Jones G.M., Trounson A.O., Gardner D.K., Kausche A., Lolatgis N., and Wood C. Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod 13 (1998) 169-177
88. Perin P.M., Malif M., Januário D.A.N.F., and Saldiva P.H.N. Comparison of the efficacy of two commercially available media for culturing one-cell embryos in the in vitro fertilization mouse model. Fertil Steril (2008) Published online 25 January 2008 [Epub ahead of print]
89. Gardner D.K., Reed L., Linck D., Sheehan C., and Lane M. Quality control in human in vitro fertilization. Semin Reprod Med 23 (2005) 319-324
90. Niemitz E., and Feinberg A.P. Epigenetics and assisted reproductive technology: a call for investigation. Am J Hum Genet 74 (2004) 599-609
91. Young L. Human embryonic stem cell methyl cycle enzyme expression: modeling programming in assisted reproduction. Reprod BioMed Online 10 (2005) 755-766
92. Johnson M. The problematic in vitro embryo in the age of epigenetics. Reprod BioMed Online 10 Suppl 1 (2005) 88-96
93. DeRisi J.L., Vishwanath R., Iyer R., and Brown P.O. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278 (1997) 680-686
94. Rinaudo P., and Schultz R.M. Effects of embryo culture on global pattern of gene expression in preimplantation mouse embryos. Reproduction 128 (2004) 301-311
95. Evsikov A.V., de Vries W.N., Peaston A.E., Radford E.E., Fancher K.S., Chen F.H., et al. Systems biology of the 2-cell mouse embryo. Cytogenet Genome Res 105 (2004) 240-250