The development of fertilized human ova to the blastocyst stage in KSOM(AA) medium: is a two-step protocol necessary?

Reproductive biomedicine online

Volumn 5, Issue 2, 2002, Pages 133-140

  Biggers, John D ^a   Racowsky, Catherine ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLASTOCYST; CELL DIFFERENTIATION; COMPARATIVE STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; FEMALE; FERTILIZATION IN VITRO; HUMAN; METHODOLOGY; OOCYTE; OVULATION INDUCTION; PHYSIOLOGY; PRENATAL DEVELOPMENT; TIME; ZYGOTE;

BLASTOCYST; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA; EMBRYONIC AND FETAL DEVELOPMENT; FEMALE; FERTILIZATION IN VITRO; HUMANS; OVULATION INDUCTION; OVUM; TIME FACTORS; ZYGOTE;

EID: 0036720148     PISSN: 14726483     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1472-6483(10)61615-X     Document Type: Article

References (24)

1. B Behr TB Pool AA Milki Preliminary clinical experience with human blastocyst development in vitro without co-culture Human Reproduction 14 1999 454 457
2. JD Biggers Reflections on the culture of the preimplantation embryo International Journal of Developmental Biology 42 1998 879 884
3. JD Biggers Thoughts on embryo culture conditions Reproductive BioMedicine Online 4 suppl. 1 2002 30 38
4. JD Biggers LM Rinaldini M Webb The study of growth factors in tissue culture Symposium of the Society for Experimental Biology 11 1957 264 297
5. JD Biggers LK McGinnis M Raffin Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium Biology of Reproduction 63 2000 281 293
6. M Deng XJ Yang Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection Molecular Reproduction and Development 59 2001 38 43
7. F Devreker D Brison M Van den Bergh Human blastocyst development in vitro in EBSS with glutamine or KSOM Fertility and Sterility 70 suppl. 1 1998 S17 (abstract).
8. YM Elhassan G Wu AC Leanez Amino acid concentrations in fluids from the bovine oviduct and uterus and in KSOM-based culture media Theriogenology 55 2001 1907 1918
9. DK Gardner Mammalian embryo culture in the absence of serum or somatic cell support Cell Biology International 18 1994 1163 1179
10. DK Gardner Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture Theriogenology 49 1998 3 95
11. DK Gardner M Lane Culture and selection of viable blastocyst: a feasible proposition for human IVF? Human Reproduction Update 3 1997 367 382
12. DK Gardner TB Pool M Lane Embryo nutrition and energy metabolism and its relationship to embryo growth, differentiation, and viability Seminars in Reproductive Medicine 18 2000 205 218
13. Y Ho K Wigglesworth JJ Eppig Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression Molecular Reproduction and Development 41 1995 232 238
14. MC Lavoir J Conaghan RA Pedersen Culture of human embryos for studies on the derivation of human pluripotent cells: a preliminary investigation Reproduction, Fertility and Development 10 1998 557 561
15. JA Lawitts JD Biggers Optimization of mouse embryo culture media using simplex methods Journal of Reproduction and Fertility 91 1991 543 556
16. HJ Leese Metabolism of the preimplantation mammalian embryo Oxford Reviews of Reproductive Biology 13 1991 35 72
17. HJ Leese Human embryo culture: back to nature Journal of Assisted Reproduction and Genetics 15 1998 466 468
18. Z Liu RH Foote Sodium chloride, osmolyte, and osmolarity effects on blastocyst formation in bovine embryos produced by in vitro fertilization (IVF) and cultured in simple serum-free media Journal of Assisted Reproduction and Genetics 13 1996 562 568
19. Z Liu RH Foote Effects of amino acids and alpha-amanitin on bovine embryo development in a simple protein-free medium Molecular Reproduction and Development 46 1997 278 285
20. Z Liu RH Foote ME Simkin Effects of amino acids and alpha-amanitin on the development of rabbit embryos in modified protein-free KSOM with HEPES Molecular Reproduction and Development 45 1996 157 162
21. Z Machaty BN Day RS Prather Development of early porcine embryos in vitro and in vivo Biology of Reproduction 59 1998 451 455
22. C Mehta N Patel StatXact 5 for Windows 2001 CATTAIL Software Corporation Cambridge, MA, USA
23. AL Smith Blastocyst culture in human IVF: the final destination or a stop along the way? Theriogenology 57 2002 97 107
24. AM Weston DP Wolf Differential preimplantation development of rhesus monkey embryos in serum-supplemented media Molecular Reproduction and Development 44 1996 88 92