Biochemical characterization of engineered amylopullulanase from Thermoanaerobacter ethanolicus 39E-implicating the non-necessity of its 100 C-terminal amino acid residues

Extremophiles

Volumn 12, Issue 5, 2008, Pages 641-650

  Lin, Hsu Yang ^a   Chuang, Hsu Han ^a   Lin, Fu Pang ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Amylopullulanase; C terminal truncation; Circular dichroism; Thermoanaerobacter ethanolicus 39E; Thermostability

Indexed keywords

AMYLOPULLULANASE; BACTERIAL PROTEIN; GLYCOSIDASE; PRIMER DNA; RECOMBINANT PROTEIN; STARCH;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN DENATURATION; RESTRICTION MAPPING; SPECTROPHOTOMETRY; THERMOANAEROBACTER; THERMODYNAMICS; THIN LAYER CHROMATOGRAPHY;

BACTERIAL PROTEINS; CHROMATOGRAPHY, THIN LAYER; CLONING, MOLECULAR; DNA PRIMERS; ENZYME STABILITY; GENETIC ENGINEERING; GLYCOSIDE HYDROLASES; KINETICS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN DENATURATION; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SPECTROPHOTOMETRY; STARCH; THERMOANAEROBACTER; THERMODYNAMICS;

THERMOANAEROBACTER; THERMOANAEROBACTER ETHANOLICUS ATCC 33223;

EID: 50649108593     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-008-0168-4     Document Type: Article

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