Aromatic residues within the substrate-binding cleft of Bacillus circulans chitinase A1 are essential for hydrolysis of crystalline chitin

Biochemical Journal

Volumn 376, Issue 1, 2003, Pages 237-244

  Watanabe, Takeshi ^a   Ariga, Yumiko ^a   Sato, Urara ^a   Toratani, Tadayuki ^a   Hashimoto, Masayuki ^a   Nikaidou, Naoki ^a   Kezuka, Yuichiro ^b   Nonaka, Takamasa ^b   Sugiyama, Junji ^c  

^a NIIGATA UNIVERSITY   (Japan)

^b NAGAOKA UNIVERSITY OF TECHNOLOGY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Aromatic amino acid; Catalytic domain; Crystalline chitin hydrolysis; Site directed mutagenesis; Substrate binding cleft

Indexed keywords

AROMATIC COMPOUNDS; CATALYSIS; COLLOIDS; CRYSTALLINE MATERIALS; ENZYMES; HYDROLYSIS; MUTAGENESIS;

MUTATIONS; OLIGOSACCHARIDES;

BIOCHEMISTRY;

AROMATIC COMPOUND; CHITIN; CHITINASE; CHITINASE A1; CRYSTALLIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS CIRCULANS; CATALYSIS; CATALYST; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE MUTATION; HYDROLYSIS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; REDUCTION; WILD TYPE;

AMINO ACIDS, AROMATIC; BACILLUS; BINDING SITES; CATALYTIC DOMAIN; CHITIN; CHITINASE; CRYSTALLIZATION; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; TRYPTOPHAN; TYROSINE;

BACILLUS CIRCULANS;

EID: 0344825227     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030419     Document Type: Article

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