Phe120 contributes to the regiospecificity of cytochrome P450 2D6: Mutation leads to the formation of a novel dextromethorphan metabolite

Biochemical Journal

Volumn 380, Issue 2, 2004, Pages 353-360

  Flanagan, Jack U ^a   Maréchal, Jean Didier ^b   Ward, Richard ^b   Kemp, Carol A ^b   McLaughlin, Lesley A ^a   Sutcliffe, Michael J ^b   Roberts, Gordon C K ^b   Paine, Mark J I ^a   Wolf, C Roland ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Cytochrome P450; Cytochrome P450 2D6 (CYP2D6); Dextromethorphan; Drug metabolism; Ligand binding property; Mutagenesis

Indexed keywords

CHEMICAL BONDS; METABOLITES; OXIDATION; SUBSTITUTION REACTIONS; SUBSTRATES;

DEMETHYLATION; REGIOSPECIFICITY;

BIOCHEMISTRY;

ALANINE; BUFURALOL; CYTOCHROME P450 2D6; DEXTROMETHORPHAN; DRUG METABOLITE; HISTIDINE; LEUCINE; METHIONINE; PHENYLALANINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; SERINE; TRYPTOPHAN; TYROSINE; BUFURALOL 1' HYDROXYLASE; BUFURALOL 1'-HYDROXYLASE; CYTOCHROME P450; ISOENZYME; MIXED FUNCTION OXIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL MEMBRANE; DEMETHYLATION; DRUG PROTEIN BINDING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HYDROXYLATION; LIGAND BINDING; MUTATION; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEREOSPECIFICITY; BIOLOGICAL MODEL; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; HUMAN; KINETICS; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PHYSIOLOGY; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS);

CYTOCHROME P-450 CYP2D6; CYTOCHROME P-450 ENZYME SYSTEM; DEXTROMETHORPHAN; HUMANS; ISOENZYMES; KINETICS; MIXED FUNCTION OXYGENASES; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHENYLALANINE; SUBSTRATE SPECIFICITY;

EID: 5044245324     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040062     Document Type: Article

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