Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 26, 2008, Pages 8956-8961

  Ibarra, Arkaitz ^a   Schwob, Etienne ^b   Méndez, Juan ^a  

^a Centro Nacional de Investigaciones Oncológicas   (Spain)

^b INSTITUT DE GÉNÉTIQUE MOLÉCULAIRE DE MONTPELLIER   (France)

Author keywords

DNA combing; DNA replication; Origin licensing

Indexed keywords

CAFFEINE; DNA; MINICHROMOSOME MAINTENANCE PROTEIN 2; MINICHROMOSOME MAINTENANCE PROTEIN 7; RNA; TRANSCRIPTION FACTOR;

ARTICLE; CELL STRESS; CELL VIABILITY; CHROMOSOME ABERRATION; CONTROLLED STUDY; DNA DAMAGE; DNA REPLICATION; HUMAN; HUMAN CELL; KARYOTYPE; MITOSIS; PRIORITY JOURNAL; RNA INTERFERENCE; CELL CYCLE S PHASE; CELL PROLIFERATION; CHROMOSOMAL INSTABILITY; DNA REPLICATION ORIGIN; HELA CELL; METABOLISM;

CELL PROLIFERATION; CHROMOSOMAL INSTABILITY; DNA DAMAGE; DNA REPLICATION; HELA CELLS; HUMANS; REPLICATION ORIGIN; S PHASE; TRANSCRIPTION FACTORS;

EID: 48249084972     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0803978105     Document Type: Article

References (33)

1. Mendez J, Stillman B (2003) Perpetuating the double helix: Molecular machines at eukaryotic DNA replication origins. BioEssays 25:1158-1167.
2. Sclafani RA, Holzen TM (2007) Cell cycle regulation of DNA replication. Annu Rev Genet 41:237-280.
3. Aparicio T, Ibarra A, Mendez J (2006) Cdc45-MCM-GINS, a new power player for DNA replication. Cell Div 1:18.
4. Moyer SE, Lewis PW, Botchan MR (2006) Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proc Natl Acad Sci USA 103:10236-10241.
5. Lengronne A, Schwob E (2002) The yeast CDK inhibitor Sic1 prevents genomic instability by promoting replication origin licensing in late G(1). Mol Cell 9:1067-1078.
6. Tanaka S, Diffley JF (2002) Deregulated G1-cyclin expression induces genomic instability by preventing efficient pre-RC formation. Genes Dev 16:2639-2649.
7. Ekholm-Reed S, et al. (2004) Deregulation of cyclin E in human cells interferes with prereplication complex assembly. J Cell Biol 165:789-800.
8. Spruck CH, Won KA, Reed SI (1999) Deregulated cyclin E induces chromosome instability. Nature 401:297-300.
9. Malumbres M, Barbacid M (2001) To cycle or not to cycle: A critical decision in cancer. Nat Rev Cancer 1:222-231.
10. Di Micco R, et al. (2006) Oncogene-induced senescence is a DNA damage response triggered by DNA hyper-replication. Nature 444:638-642.
11. Crevel G, et al. (2007) Differential requirements for MCM proteins in DNA replication in Drosophila S2 cells. PLoS ONE 2:e833.
12. Lei M, Kawasaki Y, Tye BK (1996) Physical interactions among MCM proteins and effects of MCM dosage on DNA replication in Saccharomyces cerevisiae. Mol Cell Biol 16:5081-5090.
13. Oehlmann M, Score AJ, Blow JJ (2004) The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation. J Cell Biol 165:181-190.
14. Hyrien O, Marheineke K, Goldar A (2003) Paradoxes of eukaryotic DNA replication: MCM proteins and the random completion problem. BioEssays 25:116-125.
15. Takahashi TS, Wigley DB, Walter JC (2005) Pumps, paradoxes and ploughshares: Mechanism of the MCM2-7 DNA helicase. Trends Biochem Sci 30:437-444.
16. Woodward AM, et al. (2006) Excess MCM2-7 license dormant origins of replication that can be used under conditions of replicative stress. J Cell Biol 173:673-683.
17. Feng D, Tu Z, Wu W, Liang C (2003) Inhibiting the expression of DNA replication-initiation proteins induces apoptosis in human cancer cells. Cancer Res 63:7356-7364.
18. Zou L, Liu D, Elledge SJ (2003) Replication protein A-mediated recruitment and activation of Rad17 complexes. Proc Natl Acad Sci USA 100:13827-13832.
19. Maya-Mendoza A, et al. (2007) Chk1 regulates the density of active replication origins during the vertebrate S phase. EMBO J 26:2719-2731.
20. Syljuasen RG, et al. (2005) Inhibition of human Chk1 causes increased initiation of DNA replication, phosphorylation of ATR targets, and DNA breakage. Mol Cell Biol 25:3553-3562.
21. Laskey RA, Madine MA (2003) A rotary pumping model for helicase function of MCM proteins at a distance from replication forks. EMBO Rep 4:26-30.
22. Snyder M, He W, Zhang JJ (2005) The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation. Proc Natl Acad Sci USA 102:14539-14544.
23. Cortez D, Glick G, Elledge SJ (2004) Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc Natl Acad Sci USA 101:10078-10083.
24. Tsao CC, Geisen C, Abraham RT (2004) Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling. EMBO J 23:4660-4669.
25. Marheineke K, Hyrien O (2004) Control of replication origin density and firing time in Xenopus egg extracts: role of a caffeine-sensitive, ATR-dependent checkpoint. J Biol Chem 279:28071-28081.
26. Shechter D, Costanzo V, Gautier J (2004) ATR and ATM regulate the timing of DNA replication origin firing. Nat Cell Biol 6:648-655.
27. Meister P, et al. (2007) Replication foci dynamics: replication patterns are modulated by S-phase checkpoint kinases in fission yeast. EMBO J 26:1315-1326.
28. Feijoo C, et al. (2001) Activation of mammalian Chk1 during DNA replication arrest: A role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing. J Cell Biol 154:913-923.
29. Ge XG, Jackson DA, Blow JJ (2008) Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress. Genes Dev 21:3331-3341.
30. Shima N, et al. (2007) A viable allele of Mcm4 causes chromosome instability and mammary adenocarcinomas in mice. Nat Genet 39:93-98.
31. Pruitt SC, Bailey KJ, Freeland A (2007) Reduced MCM2 expression results in severe stem/progenitor cell deficiency and cancer. Stem Cells 25:3121-3132.
32. Mendez J, Stillman B (2000) Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: Assembly of prereplication complexes in late mitosis. Mol Cell Biol 20:8602-8612.
33. Michalet X, et al. (1997) Dynamic molecular combing: Stretching the whole human genome for high-resolution studies. Science 277:1518-1523.