Gating the pore of P2X receptor channels

Nature Neuroscience

Volumn 11, Issue 8, 2008, Pages 883-887

  Li, Mufeng ^a   Chang, Tsg Hui ^a   Silberberg, Shai D ^a   Swartz, Kenton J ^a  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; COMPLEMENTARY DNA; CYSTEINE; GLUTAMATE RECEPTOR; NICOTINIC RECEPTOR; PURINERGIC P2X RECEPTOR; TETRAMER; THIOL DERIVATIVE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL STRAIN HEK293; CHANNEL GATING; DNA SEQUENCE; ELECTROPHYSIOLOGY; GENETIC TRANSFECTION; INFLAMMATION; ION CONDUCTANCE; MATHEMATICAL ANALYSIS; MEMBRANE POTENTIAL; MEMBRANE STRUCTURE; NERVE CONDUCTION; NONHUMAN; PAIN; PRIORITY JOURNAL; RAT; SIGNAL TRANSDUCTION; SYNAPTIC TRANSMISSION;

AMINO ACID SUBSTITUTION; ANIMALS; CADMIUM COMPOUNDS; CELL LINE; CELL MEMBRANE; CYSTEINE; HUMANS; ION CHANNEL GATING; KIDNEY; MESYLATES; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; PATCH-CLAMP TECHNIQUES; PROTEIN STRUCTURE, TERTIARY; RATS; RECEPTORS, PURINERGIC P2; SILVER NITRATE; TRANSFECTION;

EID: 48149111510     PISSN: 10976256     EISSN: None     Source Type: Journal    
DOI: 10.1038/nn.2151     Document Type: Article

References (36)

1. Khakh, B.S. & North, R.A. P2X receptors as cell-surface ATP sensors in health and disease. Nature 442, 527-532 (2006).
2. North, R.A. Molecular physiology of P2X receptors. Physiol. Rev. 82, 1013-1067 (2002).
3. 3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels. EMBO J. 17, 3016-3028 (1998).
4. 2 receptor: estimates based on methanethiosulfonate block at T336C. Mol. Pharmacol. 56, 973-981 (1999).
5. Jiang, L.H. et al. Subunit arrangement in P2X receptors. J. Neurosci. 23, 8903-8910 (2003).
6. 1+2 receptor subtypes. J. Mol. Biol. 342, 333-343 (2004).
7. Barrera, N.P., Ormond, S.J., Henderson, R.M., Murrell-Lagnado, R.D. & Edwardson, J.M. Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers, but that P2X6 receptor subunits do not oligomerize. J. Biol. Chem. 280, 10759-10765 (2005).
8. Evans, R.J. Orthosteric and allosteric binding sites of P2X receptors. Eur. Biophys. J. published online, doi: 10.1007/s00249-008-0275-2 (5 February 2008).
9. 4 receptor channels. J. Gen. Physiol. 125, 347-359 (2005).
10. 2 receptor. J. Neurosci. 24, 7378-7386 (2004).
11. Holmgren, M., Liu, Y., Xu, Y. & Yellen, G. On the use of thiol-modifying agents to determine channel topology. Neuropharmacology 35, 797-804 (1996).
12. + channel. Neuron 19, 175-184 (1997).
13. + channel. Neuron 32, 649-656 (2001).
14. Haines, W.R., Voigt, M.M., Migita, K., Torres, G.E. & Egan, T.M. On the contribution of the first transmembrane domain to whole-cell current through an ATP-gated ionotropic P2X receptor. J. Neurosci. 21, 5885-5892 (2001).
15. 2 receptors identified by the substituted cysteine accessibility method. J. Neurosci. 18, 2350-2359 (1998).
16. Rassendren, F., Buell, G., Newbolt, A., North, R.A. & Surprenant, A. Identification of amino acid residues contributing to the pore of a P2X receptor. EMBO J. 16, 3446-3454 (1997).
17. 2 receptor. J. Biol. Chem. 276, 14902-14908 (2001).
18. 1 receptor form disulfide bonds and are involved in receptor trafficking to the cell surface. Mol. Pharmacol. 61, 303-311 (2002).
19. 2 purinoceptor. J. Neurosci. 22, 3873-3880 (2002).
20. Silberberg, S.D., Li, M. & Swartz, K.J. Ivermectin interaction with transmembrane helices reveals widespread rearrangements during opening of P2X receptor channels. Neuron 54, 263-274 (2007).
21. Stauffer, D.A. & Karlin, A. Electrostatic potential of the acetylcholine binding sites in the nicotinic receptor probed by reactions of binding-site cysteines with charged methanethiosulfonates. Biochemistry 33, 6840-6849 (1994).
22. Armstrong, C.M. Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axons. J. Gen. Physiol. 58, 413-437 (1971).
23. Armstrong, C.M. Ionic pores, gates and gating currents. Q. Rev. Biophys. 7, 179-210 (1974).
24. Choi, K.L., Mossman, C., Aube, J. & Yellen, G. The internal quaternary ammonium receptor site of Shaker potassium channels. Neuron 10, 533-541 (1993).
25. Cao, L., Young, M.T., Broomhead, H.E., Fountain, S.J. & North, R.A. Thr339-to-serine substitution in rat P2X2 receptor second transmembrane domain causes constitutive opening and indicates a gating role for Lys308. J. Neurosci. 27, 12916-12923 (2007).
26. Flynn, G.E. & Zagotta, W.N. Conformational changes in S6 coupled to the opening of cyclic nucleotide-gated channels. Neuron 30, 689-698 (2001).
27. Contreras, J.E., Srikumar, D. & Holmgren, M. Gating at the selectivity filter in cyclic nucleotide-gated channels. Proc. Natl. Acad. Sci. USA 105, 3310-3314 (2008).
28. Lu, Q. & Miller, C. Silver as a probe of pore-forming residues in a potassium channel. Science 268, 304-307 (1995).
29. Jasti, J., Furukawa, H., Gonzales, E.B. & Gouaux, E. Structure of acid-sensing ion channel 1 at 1.9-Å resolution and low pH. Nature 449, 316-323 (2007).
30. + conduction and selectivity. Science 280, 69-77 (1998).
31. 2 receptor. J. Biol. Chem. 276, 30934-30941 (2001).
32. Egan, T.M. & Khakh, B.S. Contribution of calcium ions to P2X channel responses. J. Neurosci. 24, 3413-3420 (2004).
33. Yellen, G. The voltage-gated potassium channels and their relatives. Nature 419, 35-42 (2002).
34. + channel-Fab complex at 2.0-Å resolution. Nature 414, 43-48 (2001).
35. Brake, A.J., Wagenbach, M.J. & Julius, D. New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor. Nature 371, 519-523 (1994).
36. Hamill, O.P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391, 85-100 (1981).