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Volumn 182, Issue 1, 2008, Pages 129-140

Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy

Author keywords

[No Author keywords available]

Indexed keywords

AUTOPHAGY RELATED PROTEIN 11; AUTOPHAGY RELATED PROTEIN 8; AUTOPHAGY RELATED PROTEIN 9; PROTEIN;

EID: 47549086520     PISSN: 00219525     EISSN: 00219525     Source Type: Journal    
DOI: 10.1083/jcb.200711112     Document Type: Article
Times cited : (100)

References (49)
  • 1
    • 0022753540 scopus 로고
    • PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors
    • Ammerer, G., C.P. Hunter, J.H. Rothman, G.C. Saari, L.A. Valls, and T.H. Stevens. 1986. PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol. Cell. Biol. 6:2490-2499.
    • (1986) Mol. Cell. Biol , vol.6 , pp. 2490-2499
    • Ammerer, G.1    Hunter, C.P.2    Rothman, J.H.3    Saari, G.C.4    Valls, L.A.5    Stevens, T.H.6
  • 2
    • 0031417385 scopus 로고    scopus 로고
    • Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/ lysosome
    • Baba, M., M. Osumi, S.V. Scott, D.J. Klionsky, and Y. Ohsumi. 1997. Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/ lysosome. J. Cell Biol. 139:1687-1695.
    • (1997) J. Cell Biol , vol.139 , pp. 1687-1695
    • Baba, M.1    Osumi, M.2    Scott, S.V.3    Klionsky, D.J.4    Ohsumi, Y.5
  • 3
    • 0032101334 scopus 로고    scopus 로고
    • The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    • Babst, M., B. Wendland, E.J. Estepa, and S.D. Emr. 1998. The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J. 17:2982-2993.
    • (1998) EMBO J , vol.17 , pp. 2982-2993
    • Babst, M.1    Wendland, B.2    Estepa, E.J.3    Emr, S.D.4
  • 4
    • 0036696804 scopus 로고    scopus 로고
    • Escrt-III: An endosome-associated heterooligomeric protein complex required for mvb sorting
    • Babst, M., D.J. Katzmann, E.J. Estepa-Sabal, T. Meerloo, and S.D. Emr. 2002. Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev. Cell. 3:271-282.
    • (2002) Dev. Cell , vol.3 , pp. 271-282
    • Babst, M.1    Katzmann, D.J.2    Estepa-Sabal, E.J.3    Meerloo, T.4    Emr, S.D.5
  • 5
    • 2442645050 scopus 로고    scopus 로고
    • The Ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae
    • Budovskaya, Y.V., J.S. Stephan, F. Reggiori, D.J. Klionsky, and P.K. Herman. 2004. The Ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae. J. Biol. Chem. 279:20663-20671.
    • (2004) J. Biol. Chem , vol.279 , pp. 20663-20671
    • Budovskaya, Y.V.1    Stephan, J.S.2    Reggiori, F.3    Klionsky, D.J.4    Herman, P.K.5
  • 7
    • 39449108917 scopus 로고    scopus 로고
    • The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae
    • Cheong, H., U. Nair, J. Geng, and D.J. Klionsky. 2007. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae. Mol. Biol. Cell. 19:668-681.
    • (2007) Mol. Biol. Cell , vol.19 , pp. 668-681
    • Cheong, H.1    Nair, U.2    Geng, J.3    Klionsky, D.J.4
  • 9
    • 43949143804 scopus 로고    scopus 로고
    • The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy
    • Fujita, N., T. Itoh, M. Fukuda, T. Noda, and T. Yoshimori. 2008. The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy. Mol. Biol. Cell. 19:2092-2100.
    • (2008) Mol. Biol. Cell , vol.19 , pp. 2092-2100
    • Fujita, N.1    Itoh, T.2    Fukuda, M.3    Noda, T.4    Yoshimori, T.5
  • 11
    • 0028800171 scopus 로고
    • Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway
    • Harding, T.M., K.A. Morano, S.V. Scott, and D.J. Klionsky. 1995. Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway. J. Cell Biol. 131:591-602.
    • (1995) J. Cell Biol , vol.131 , pp. 591-602
    • Harding, T.M.1    Morano, K.A.2    Scott, S.V.3    Klionsky, D.J.4
  • 12
    • 33845692364 scopus 로고    scopus 로고
    • Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
    • He, C., H. Song, T. Yorimitsu, I. Monastyrska, W.-L. Yen, J.E. Legakis, and D.J. Klionsky. 2006. Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. J. Cell Biol. 175:925-935.
    • (2006) J. Cell Biol , vol.175 , pp. 925-935
    • He, C.1    Song, H.2    Yorimitsu, T.3    Monastyrska, I.4    Yen, W.-L.5    Legakis, J.E.6    Klionsky, D.J.7
  • 13
    • 34548265278 scopus 로고    scopus 로고
    • Autophagy and human disease
    • Huang, J., and D.J. Klionsky. 2007. Autophagy and human disease. Cell Cycle. 6:1837-1849.
    • (2007) Cell Cycle , vol.6 , pp. 1837-1849
    • Huang, J.1    Klionsky, D.J.2
  • 14
    • 0034050457 scopus 로고    scopus 로고
    • The itinerary of a vesicle component, Aut7p/Cvt5p, terminates in the yeast vacuole via the autophagy/Cvt pathways
    • Huang, W.-P., S.V. Scott, J. Kim, and D.J. Klionsky. 2000. The itinerary of a vesicle component, Aut7p/Cvt5p, terminates in the yeast vacuole via the autophagy/Cvt pathways. J. Biol. Chem. 275:5845-5851.
    • (2000) J. Biol. Chem , vol.275 , pp. 5845-5851
    • Huang, W.-P.1    Scott, S.V.2    Kim, J.3    Klionsky, D.J.4
  • 15
    • 0035827541 scopus 로고    scopus 로고
    • Vacuolar localization of oligomeric α-mannosidase requires the cytoplasm to vacuole targeting and autophagy pathway components in Saccharomyces cerevisiae
    • Hutchins, M.U., and D.J. Klionsky. 2001. Vacuolar localization of oligomeric α-mannosidase requires the cytoplasm to vacuole targeting and autophagy pathway components in Saccharomyces cerevisiae. J. Biol. Chem. 276:20491-20498.
    • (2001) J. Biol. Chem , vol.276 , pp. 20491-20498
    • Hutchins, M.U.1    Klionsky, D.J.2
  • 19
    • 0035825175 scopus 로고    scopus 로고
    • Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex
    • Kim, J., W.-P. Huang, and D.J. Klionsky. 2001a. Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J. Cell Biol. 152:51-64.
    • (2001) J. Cell Biol , vol.152 , pp. 51-64
    • Kim, J.1    Huang, W.-P.2    Klionsky, D.J.3
  • 21
    • 0037016752 scopus 로고    scopus 로고
    • Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation
    • Kim, J., W.-P. Huang, P.E. Stromhaug, and D.J. Klionsky. 2002. Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation. J. Biol. Chem. 277:763-773.
    • (2002) J. Biol. Chem , vol.277 , pp. 763-773
    • Kim, J.1    Huang, W.-P.2    Stromhaug, P.E.3    Klionsky, D.J.4
  • 23
    • 0026640551 scopus 로고
    • Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway
    • Klionsky, D.J., R. Cueva, and D.S. Yaver. 1992. Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway. J. Cell Biol. 119:287-299.
    • (1992) J. Cell Biol , vol.119 , pp. 287-299
    • Klionsky, D.J.1    Cueva, R.2    Yaver, D.S.3
  • 24
    • 0037166241 scopus 로고    scopus 로고
    • Formation of the approximately 350-kDa Apg12-Apg5·Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast
    • Kuma, A., N. Mizushima, N. Ishihara, and Y. Ohsumi. 2002. Formation of the approximately 350-kDa Apg12-Apg5·Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J. Biol. Chem. 277:18619-18625.
    • (2002) J. Biol. Chem , vol.277 , pp. 18619-18625
    • Kuma, A.1    Mizushima, N.2    Ishihara, N.3    Ohsumi, Y.4
  • 25
    • 0032785638 scopus 로고    scopus 로고
    • Copper ion inducible and repressible promoter systems in yeast
    • Labbé, S., and D.J. Thiele. 1999. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306:145-153.
    • (1999) Methods Enzymol , vol.306 , pp. 145-153
    • Labbé, S.1    Thiele, D.J.2
  • 27
    • 0031820288 scopus 로고    scopus 로고
    • Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
    • Longtine, M.S., A. McKenzie III, D.J. Demarini, N.G. Shah, A. Wach, A. Brachat, P. Philippsen, and J.R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast. 14:953-961.
    • (1998) Yeast , vol.14 , pp. 953-961
    • Longtine, M.S.1    McKenzie III, A.2    Demarini, D.J.3    Shah, N.G.4    Wach, A.5    Brachat, A.6    Philippsen, P.7    Pringle, J.R.8
  • 28
    • 3042645379 scopus 로고    scopus 로고
    • Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae
    • Luo, J., E.A. Vallen, C. Dravis, S.E. Tcheperegine, B. Drees, and E. Bi. 2004. Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J. Cell Biol. 165:843-855.
    • (2004) J. Cell Biol , vol.165 , pp. 843-855
    • Luo, J.1    Vallen, E.A.2    Dravis, C.3    Tcheperegine, S.E.4    Drees, B.5    Bi, E.6
  • 29
    • 27644493346 scopus 로고    scopus 로고
    • The pleiotropic role of autophagy: From protein metabolism to bactericide
    • Mizushima, N. 2005. The pleiotropic role of autophagy: from protein metabolism to bactericide. Cell Death Differ. 12:1535-1541.
    • (2005) Cell Death Differ , vol.12 , pp. 1535-1541
    • Mizushima, N.1
  • 30
    • 34447099450 scopus 로고    scopus 로고
    • Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion
    • Nakatogawa, H., Y. Ichimura, and Y. Ohsumi. 2007. Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion. Cell. 130:165-178.
    • (2007) Cell , vol.130 , pp. 165-178
    • Nakatogawa, H.1    Ichimura, Y.2    Ohsumi, Y.3
  • 31
    • 0037119448 scopus 로고    scopus 로고
    • Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the preautophagosomal structure is required for selective autophagy
    • Nice, D.C., T.K. Sato, P.E. Stromhaug, S.D. Emr, and D.J. Klionsky. 2002. Cooperative binding of the cytoplasm to vacuole targeting pathway proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the preautophagosomal structure is required for selective autophagy. J. Biol. Chem. 277:30198-30207.
    • (2002) J. Biol. Chem , vol.277 , pp. 30198-30207
    • Nice, D.C.1    Sato, T.K.2    Stromhaug, P.E.3    Emr, S.D.4    Klionsky, D.J.5
  • 32
    • 0029036915 scopus 로고
    • Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae
    • Noda, T., A. Matsuura, Y. Wada, and Y. Ohsumi. 1995. Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 210:126-132.
    • (1995) Biochem. Biophys. Res. Commun , vol.210 , pp. 126-132
    • Noda, T.1    Matsuura, A.2    Wada, Y.3    Ohsumi, Y.4
  • 33
    • 0346503885 scopus 로고    scopus 로고
    • The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the preautophagosomal structure
    • Reggiori, F., K.A. Tucker, P.E. Stromhaug, and D.J. Klionsky. 2004. The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the preautophagosomal structure. Dev. Cell. 6:79-90.
    • (2004) Dev. Cell , vol.6 , pp. 79-90
    • Reggiori, F.1    Tucker, K.A.2    Stromhaug, P.E.3    Klionsky, D.J.4
  • 34
    • 27644544004 scopus 로고    scopus 로고
    • Atg9 cycles between mitochondria and the pre-autophagosomal structure in yeasts
    • Reggiori, F., T. Shintani, U. Nair, and D. Klionsky. 2005. Atg9 cycles between mitochondria and the pre-autophagosomal structure in yeasts. Autophagy. 1:101-109.
    • (2005) Autophagy , vol.1 , pp. 101-109
    • Reggiori, F.1    Shintani, T.2    Nair, U.3    Klionsky, D.4
  • 35
    • 0023739386 scopus 로고
    • Protein sorting in Saccharomyces cerevisiae: Isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases
    • Robinson, J.S., D.J. Klionsky, L.M. Banta, and S.D. Emr. 1988. Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol. Cell. Biol. 8:4936-4948.
    • (1988) Mol. Cell. Biol , vol.8 , pp. 4936-4948
    • Robinson, J.S.1    Klionsky, D.J.2    Banta, L.M.3    Emr, S.D.4
  • 36
    • 0029913505 scopus 로고    scopus 로고
    • Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole
    • Scott, S.V., A. Hefner-Gravink, K.A. Morano, T. Noda, Y. Ohsumi, and D.J. Klionsky. 1996. Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole. Proc. Natl. Acad. Sci. USA. 93:12304-12308.
    • (1996) Proc. Natl. Acad. Sci. USA , vol.93 , pp. 12304-12308
    • Scott, S.V.1    Hefner-Gravink, A.2    Morano, K.A.3    Noda, T.4    Ohsumi, Y.5    Klionsky, D.J.6
  • 37
    • 0034964443 scopus 로고    scopus 로고
    • Cvt19 is a receptor for the cytoplasm-to-vacuole targeting pathway
    • Scott, S.V., J. Guan, M.U. Hutchins, J. Kim, and D.J. Klionsky. 2001. Cvt19 is a receptor for the cytoplasm-to-vacuole targeting pathway. Mol. Cell. 7:1131-1141.
    • (2001) Mol. Cell , vol.7 , pp. 1131-1141
    • Scott, S.V.1    Guan, J.2    Hutchins, M.U.3    Kim, J.4    Klionsky, D.J.5
  • 38
    • 3042722112 scopus 로고    scopus 로고
    • Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
    • Sheff, M.A., and K.S. Thorn. 2004. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 21:661-670.
    • (2004) Yeast , vol.21 , pp. 661-670
    • Sheff, M.A.1    Thorn, K.S.2
  • 39
    • 8344242220 scopus 로고    scopus 로고
    • Autophagy in health and disease: A double-edged sword
    • Shintani, T., and D.J. Klionsky. 2004a. Autophagy in health and disease: a double-edged sword. Science. 306:990-995.
    • (2004) Science , vol.306 , pp. 990-995
    • Shintani, T.1    Klionsky, D.J.2
  • 40
    • 3142677196 scopus 로고    scopus 로고
    • Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway
    • Shintani, T., and D.J. Klionsky. 2004b. Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway. J. Biol. Chem. 279:29889-29894.
    • (2004) J. Biol. Chem , vol.279 , pp. 29889-29894
    • Shintani, T.1    Klionsky, D.J.2
  • 41
    • 0036901104 scopus 로고    scopus 로고
    • Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway
    • Shintani, T., W.-P. Huang, P.E. Stromhaug, and D.J. Klionsky. 2002. Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway. Dev. Cell. 3:825-837.
    • (2002) Dev. Cell , vol.3 , pp. 825-837
    • Shintani, T.1    Huang, W.-P.2    Stromhaug, P.E.3    Klionsky, D.J.4
  • 42
    • 0035503594 scopus 로고    scopus 로고
    • The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation
    • Suzuki, K., T. Kirisako, Y. Kamada, N. Mizushima, T. Noda, and Y. Ohsumi. 2001. The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J. 20:5971-5981.
    • (2001) EMBO J , vol.20 , pp. 5971-5981
    • Suzuki, K.1    Kirisako, T.2    Kamada, Y.3    Mizushima, N.4    Noda, T.5    Ohsumi, Y.6
  • 43
    • 33846514235 scopus 로고    scopus 로고
    • Hierarchy of Atg proteins in pre-autophagosomal structure organization
    • Suzuki, K., Y. Kubota, T. Sekito, and Y. Ohsumi. 2007. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes Cells. 12:209-218.
    • (2007) Genes Cells , vol.12 , pp. 209-218
    • Suzuki, K.1    Kubota, Y.2    Sekito, T.3    Ohsumi, Y.4
  • 44
    • 0026668042 scopus 로고
    • Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction
    • Takeshige, K., M. Baba, S. Tsuboi, T. Noda, and Y. Ohsumi. 1992. Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction. J. Cell Biol. 119:301-311.
    • (1992) J. Cell Biol , vol.119 , pp. 301-311
    • Takeshige, K.1    Baba, M.2    Tsuboi, S.3    Noda, T.4    Ohsumi, Y.5
  • 45
    • 26844562643 scopus 로고    scopus 로고
    • Counting cytokinesis proteins globally and locally in fission yeast
    • Wu, J.Q., and T.D. Pollard. 2005. Counting cytokinesis proteins globally and locally in fission yeast. Science. 310:310-314.
    • (2005) Science , vol.310 , pp. 310-314
    • Wu, J.Q.1    Pollard, T.D.2
  • 46
    • 47549092694 scopus 로고    scopus 로고
    • Atg8 controls phagophore expansion during autophagosome formation
    • DOI:10.1091/mbc.E07-12-1292
    • Xie, Z., U. Nair, and D.J. Klionsky. 2008. Atg8 controls phagophore expansion during autophagosome formation. Mol. Biol. Cell. DOI:10.1091/mbc.E07-12-1292.
    • (2008) Mol. Biol. Cell
    • Xie, Z.1    Nair, U.2    Klionsky, D.J.3
  • 47
    • 33846807374 scopus 로고    scopus 로고
    • Atg27 is required for autophagy-dependent cycling of Atg9
    • Yen, W.-L., J.E. Legakis, U. Nair, and D.J. Klionsky. 2006. Atg27 is required for autophagy-dependent cycling of Atg9. Mol. Biol. Cell. 18:581-593.
    • (2006) Mol. Biol. Cell , vol.18 , pp. 581-593
    • Yen, W.-L.1    Legakis, J.E.2    Nair, U.3    Klionsky, D.J.4
  • 48
    • 16344365254 scopus 로고    scopus 로고
    • Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway
    • Yorimitsu, T., and D. Klionsky. 2005a. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol. Biol. Cell. 16:1593-1605.
    • (2005) Mol. Biol. Cell , vol.16 , pp. 1593-1605
    • Yorimitsu, T.1    Klionsky, D.2
  • 49
    • 27644484061 scopus 로고    scopus 로고
    • Autophagy: Molecular machinery for self-eating
    • Yorimitsu, T., and D. Klionsky. 2005b. Autophagy: molecular machinery for self-eating. Cell Death Differ. 12:1542-1552.
    • (2005) Cell Death Differ , vol.12 , pp. 1542-1552
    • Yorimitsu, T.1    Klionsky, D.2


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