The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation

EMBO Journal

Volumn 20, Issue 21, 2001, Pages 5971-5981

  Suzuki, Kuninori ^a,b   Kirisako, Takayoshi ^a,b   Kamada, Yoshiaki ^a,b   Mizushima, Noboru ^a,c   Noda, Takeshi ^a,b   Ohsumi, Yoshinori ^a,b  

^a NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

^b GRADUATE UNIVERSITY FOR ADVANCED STUDIES   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

APG; Autophagosome; Autophagy; GFP; Membrane dynamics

Indexed keywords

MEMBRANE PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN APG; PROTEIN KINASE; UNCLASSIFIED DRUG;

ARTICLE; AUTOPHAGY; CELL VACUOLE; COMPLEX FORMATION; EUKARYOTIC CELL; GENE FUNCTION; NONHUMAN; PHAGOSOME; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; YEAST;

AUTOPHAGY; CARRIER PROTEINS; FUNGAL PROTEINS; GREEN FLUORESCENT PROTEINS; HEAT-SHOCK PROTEINS; LUMINESCENT PROTEINS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MUTAGENESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE; TRANSCRIPTION FACTORS; VACUOLES;

EUKARYOTA;

EID: 0035503594     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.21.5971     Document Type: Article

References (45)

1. Cytoplasm to vacuole trafficking of aminopeptidase I requires a t-SNARE-Seclp complex composed of Tlg2p and Vps45p
2. Dissection of autophagosome biogenesis into distinct nucleation and expansion steps
3. Ultrastructural analysis of the autophagic process in yeast: Detection of autophagosomes and their characterization
4. Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p
5. Mutagenic PCR
6. A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole
7. Ultrastructural and immunocytochemical characterization of autophagic vacuoles in isolated hepatocytes: Effects of vinblastine and asparagine on vacuole distributions
8. Ultrastructural characterization of the delimiting membranes of isolated autophagosomes and amphisomes by freeze-fracture electron microscopy
9. Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae
10. Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways
11. Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway
12. Rapid protein extraction from Saccharomyces cerevisiae
13. The itinerary of a vesicle component, Aut7p/Cvt5p, terminates in the yeast vacuole via the autophagy/Cvt pathways
14. A ubiquitin-like system mediates protein lipidation
15. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
16. Tor-mediated induction of autophagy via an Apg1 protein kinase complex
17. Structural and functional analyses of APG5, a gene involved in autophagy in yeast
18. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae
19. Two distinct Vps34 PtdIns 3-kinase complexes function in autophagy and CPY sorting in Saccharomyces cerevisiae
20. Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network
21. Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy and peroxisome degradation pathways
22. Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p and the autophagy conjugation complex
23. Formation process of autophagosome is traced with Apg8/Aut7p in yeast
24. The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
25. Vacuolar import of proteins and organelles from the cytoplasm
26. Induction of autophagy and inhibition of tumorigenesis by beclin 1
27. Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae
28. A protein conjugation system essential for autophagy
29. Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway
30. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
31. Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies
32. Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast
33. Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae
34. Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways
35. Distinct classes of phosphatidylinositol 3′-kinases are involved in signaling pathways that control macroautophagy in HT-29 cells
36. Morphological classification of the yeast vacuolar protein sorting mutants: Evidence for a prevacuolar compartment in class E vps mutants
37. Aminopeptidase I is targeted to the vacuole by a nonclassical vesicular mechanism
38. Regulation of autophagic protein degradation in isolated liver cells
39. Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast
40. Apg2p functions in autophagosome formation on the perivacuolar structure
41. Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy
42. Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae
43. A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast
44. Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein