Integrated Approaches for Detection of Plant Pathogenic Bacteria and Diagnosis of Bacterial Diseases

Annual Review of Phytopathology

Volumn 42, Issue , 2004, Pages 339-366

  Alvarez, Anne M ^a  

^a UNIVERSITY OF HAWAII   (United States)

Author keywords

DNA based detection methods; Genomic probes; Immunodiagnosis; Monoclonal antibodies; Polyphasic analysis

Indexed keywords

ANIMAL; BACTERIUM; GENETIC PROCEDURES; GENETICS; IMMUNOLOGICAL PROCEDURES; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PLANT DISEASE; REVIEW;

ANIMALS; BACTERIA; BACTERIOLOGICAL TECHNIQUES; GENETIC TECHNIQUES; IMMUNOLOGIC TECHNIQUES; PLANT DISEASES;

ANIMALIA; BACTERIA (MICROORGANISMS);

EID: 4744365810     PISSN: 00664286     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.phyto.42.040803.140329     Document Type: Review

References (145)

1. Alvarez AM. 2001. Differentiation of bacterial populations in seed extracts by flow cytometry. In Plant Pathogenic Bacteria, ed. SH De Boer, pp. 393-96. Dordrecht: Kluwer
2. Alvarez AM, Adams PD. 1999. Flow cytometry: a promising tool for seed health testing. See Ref. 90a, pp. 110-14
3. Alvarez AM, Benedict AA, Gnanamanickam SS. 1993. Identification of seed-borne bacterial pathogens of rice with monoclonal antibodies. Presented at Proc. Symp. Seed Health Test. 1st, Ottawa, Ont., Can.
4. Alvarez AM, Benedict AA, Mizumoto CY. 1985. Identification of xanthomonads and groupings of Xanthomonas campestris pv. campestris strains with monoclonal antibodies. Phytopathology 75:722-28
5. Alvarez AM, Benedict AA, Mizumoto CY, Hunter JE, Gabriel DW. 1994. Serological, pathological, and genetic diversity among strains of Xanthomonas campestris infecting crucifers. Phytopathology 84:1449-57
6. Alvarez AM, Benedict AA, Mizumoto CY, Pollard LW, Civerolo EL. 1991. Analysis of Xanthomonas campestris pv. citri and Xanthomonas campestris pv. citrumelo with monoclonal antibodies. Phytopathology 81:857-65
7. Alvarez AM, Berestecky J, Stiles JI, Ferreira SJ, Benedict AA. 1992. Serological and molecular approaches to identification of Pseudomonas solanacearum strains from Heliconia. Presented at Bact. Wilt. Int. Conf., Kaohsiung, Taiwan
8. Alvarez AM, Derie M, Benedict AA, Gabrielson R. 1993. Characteristics of a monoclonal antibody to Clavibacter michiganensis subsp. michiganensis. Phytopathology 83:1405
9. Alvarez AM, Kaneshiro WS. 1999. Detection and identification of Clavibacter michiganensis subsp. michiganensis in tomato seed. See Ref. 90a, pp. 93-97
10. Alvarez AM, Rehman FU, Leach JE. 1997. Comparison of serological and molecular methods for detection of Xanthomonas oryzae pv. oryzae in rice seed. Presented at Seed Health Test.: Progr. Towards the 21st Century, Cambridge, UK
11. Alvarez AM, Schenck S, Benedict AA. 1996. Differentiation of Xanthomonas albilineans strains with monoclonal antibody reaction patterns and DNA fingerprints. Plant Pathol. 45:358-66
12. Arias RS, Mochizuki GT, Fukui R, Alvarez AM. 1996. Most probable number method to enumerate a bioluminescent Xanthomonas campestris pv. campestris in soil. Soil Biol. Biochem. 28:1725-28
13. Avrova AO, Hyman LJ, Toth RL, Toth IK. 2002. Application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria Erwinia carotovora and Erwinia chrysanthemi. Appl. Environ. Microbiol. 68:1499-508
14. Bach HJ, Jessen I, Schloter M, Munch JC. 2003. A TaqMan-PCR Protocol for quantification and differentiation of the phytopathogenic Clavibacter michiganensis subspecies. J. Microbiol. Methods 52:85-91
15. Benedict AA, Alvarez AM, Berestecky J, Imanaka J, Mizumoto CY, et al. 1989. Monoclonal antibodies specific for Xanthomonas campestris pv. oryzae and pv. oryzicola. Phytopathology 79:322-28
16. Benedict AA, Alvarez AM, Pollard LW. 1990. Pathovar-specific antigens of Xanthomonas campestris pv. begoniae and X. campestris pv. pelargonii detected with monoclonal antibodies. Appl. Environ. Microbiol. 56:572-74
17. Bentsink L, Leone GOM, Van Beckhoven JRCM, van Schijndel HB, van Gemen B, van der Wolf JM. 2002. Amplification of RNA by NASBA allows direct detection of viable cells of Ralstonia solanacearum in potato. J. Appl. Microbiol. 93:647-55
18. Bertolini E, Olmos A, López MM, Cambra M. 2003. Multiplex nested RT-PCR in a single closed tube for sensitive and simultaneous detection of tour RNA viruses and Pseudomonas savastanoi pv. savastanoi in olive trees. Phytopathology 93:286-92
19. Bertolini E, Penyalver R, García A, Olmos A, et al. 2003. Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube. J. Microbiol. Methods 52:261-66
20. Bishop AL, Burr TJ, Millak VL, Katz BH. 1989. A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 and its utilization for indexing grapevine propagation material. Phytopathology 79:995-98
21. Bouzar H, Jones JB, Stall RE, Hodge NC, Minsavage GV, et al. 1994. Physiological, chemical, serological, and pathogenic analyses of a worldwide collection of Xanthomonas campestris pv. vesicatoria strains. Phytopathology 84:663-71
22. Bouzar H, Moore LW, Schaup HW. 1988. Lipopolysaccharide from Agrobacterium tumefaciens biovar 6 induces the production of strain-specific antibodies. Phytopathology 78:1237-41
23. Bradbury JF. 1986. Guide to Plant Pathogenic Bacteria. Farnham Royal, UK: CAB Int. Mycol. Inst. 332 pp.
24. Bragard C, Mehta YR, Maraile H. 1993. Serodiagnostic assays versus the routine techniques to detect Xanthomonas campestris pv. undulosa in wheat seeds. Fitopathol. Bras. 18:42-50
25. Bultreys A, Gheysen II. 1999. Biological and molecular detection of toxic lipodepsipeptide-producing Pseudomonas syringae strains and PCR identification in plants. Appl. Environ. Microbiol. 65:1904-9
26. Caruso P, Bertolini E, Cambra M, López MM. 2003. A new and sensitive Cooperational polymerase chain reaction (Co-PCR) for rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55:257-72
27. Caruso P, Gorris MT, Cambra M, Palomo JL, Collar J, Lopez MM. 2002. Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers. Appl. Environ. Microbiol. 68:3634-38
28. Cervantes-Martinez J, Flores-Hernandez R, Rodriguez-Garay B, Santacruz-Ruvalcaba F. 2002. Detection of bacterial infection of agave plants by laser-induced fluorescence. Appl. Opt. 41:2541-45
29. Chitarra LG, Langerak CJ, Bergervoet JH, van den Bulk RW. 2002. Detection of the plant pathogenic bacterium Xanthomonas campestris pv. campestris in seed extracts of Brassica sp. Applying fluorescent antibodies and flow cytometry. Cytometry 47:118-26
30. Cubero J, Graham JH. 2002. Genetic relationship among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers for their identification by PCR. Appl. Environ. Microbiol. 68:1257-64
31. Cubero J, Martínez MC, Llop P, López, MM. 1999. A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumors. J. Appl. Microbiol. 86:591-602
32. Danks C, Barker I. 2000. On-site detection of plant pathogens using lateral flow devices. Bull. OEPP/EPPO 30:421-26
33. De Boer SH. 1987. Monoclonal antibodies to the lipopolysaccharide of Erwinia carotovora subsp. atroseptica serogroup I. Phytopathology 77:828-32
34. De Boer SH, Wieczorek A. 1984. Production of monoclonal antibodies to Corynebacterium sepedonicum. Phytopathology 74:1431-34
35. Dow M, Neuman MA, von Roepenak E. 2000. The induction and inoculation of plant defense responses by bacterial lipopolysaccharides. Annu. Rev. Phytopathol. 38:241-61
36. Drevinek P, Hrbackova H, Cinek O, Bartosova J, Nyc O, et al. 2002. Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA. J. Clin. Microbiol. 40:3485-88
37. Duveiller E, Bragard C. 1992. Comparison of immunofluorescence and two assays for detection of Xanthomonas campestris pv. undulosa in seeds of small grains. Plant Dis. 76:999-1003
38. Elphinstone JG. 2003. Molecular methods for diagnosis of bacterial plant diseases at the Central Science Laboratory, York, U.K. Presented at Mol. Diagn. Plant Pathol., Lamezia Terme, Italy
39. Elphinstone JG, Hennessy J, Wilson JK, Stead D. 1996. Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tuber extracts. Bull. OEPP/EPPO 26:663-78
40. Franken AAJM. 1990. Production, characterization and application of monoclonal antibodies for detecting Xanthomonas campestris pv. campestris in cabbage seeds. Presented at Proc. Symp. Perspect. Monoclon. Antib. Agric., PUDOC, Wageningen, Neth.
41. Goncalves ER, Rosato YB. 2000. Genotypic characterization of xanthomonad strains isolated from passion fruit plants (Passiflora spp.) and their relatedness to different Xanthomonas species. Int. J. Syst. Evol. Microbiol. 50(Part 2):811-21
42. Goncalves ER, Rosato YB. 2002. Phylogenetic analysis of Xanthomonas species based upon 16S-23S rDNA intergenic spacer sequences. Int. J. Syst. Evol. Microbiol. 52:355-61
43. Gorris MT, Alarcon B, Lopez MM, Cambra M. 1994. Characterization of monoclonal antibodies specific for Erwinia carotovora subsp. atroseptica and comparison of serological methods for its sensitive detection on potato tubers. Appl. Environ. Microbiol. pp. 2076-85
44. Gorris MT, Camarasa E, López MM, Paulin JP, Chartier R, Cambra M. 1996. Production and characterization of monoclonal antibodies specific for Erwinia amylovora and their use in different serological techniques. Acta Hortic. 411:47-51
45. Gorris MT, Cambra M, López MM. 1989. Production of monoclonal antibodies specific to Xylophilus ampelinus. Proc. 7th.Int. Conf. Plant Pathol. Bacteriol. Budapest, ed. Z Klement, pp. 913-21
46. Griep RA, Van Twisk C, Van Beckhoven JRCM, Van der Wolf JM, Schots A. 1998. Development of specific recombinant monoclonal antibodies against the lipopolysaccharides of Ralstonia solanacearum race 3. Phytopathology 88:795-803
47. Grogan R. 1981. The science and art of plant-disease diagnosis. Annu. Rev. Phytopathol. 19:333-51
48. Gunasekera TS, Dorsch MR, Slade MB, Veal DA. 2003. Specific detection of Pseudomonas spp. in milk by fluorescence in situ hybridization using ribosomal RNA directed probes. J. Appl. Microbiol. 94:936-45
49. Guven K, Mutlu MB. 2000. Development of immunomagnetic separation technique for isolation of Pseudomonas syringae pv. phaseolicola. Fol. Microbiol. 45:321-24
50. Halk EL, de Boer SH. 1985. Monoclonal antibodies in plant disease research. Annu. Rev. Phytopathol. 23:321-50
51. Handelsman J, Raffel SJ, Sequeira L. 1990. Monoclonal antibodies against Agrobacterium strain C58. Curr. Microbiol. 21:181-85
52. Harper WJ. 2001. The strengths and weaknesses of the electronic nose. Adv. Exp. Med. Biol. 488:59-71
53. Hartung JS, Pruvost OP, Villemot I, Alvarez AM. 1996. Rapid and sensitive colorimetric detection of Xanthomonas axonopodis pv. citri by immunocapture and nested-polymerase chain reaction assay. Phytopathology 86:95-101
54. Hu FP, Young JM, Triggs CM, Park DC, Saul DJ. 2001. Relationships within the Proteobacteria of plant pathogenic Acidovorax species and subspecies, Burkholderia species, and Herbaspirillum rubrisubalbicans by sequence analysis of 16S rDNA, numerical analysis and determinative tests. Antonie Van Leeuwenhoek J. Microbiol. Serol. 80:201-14
55. Hyman LJ, Birch PR, Dellagi A, Avrova AO, Toth IK. 2000. A competitive PCR-based method for the detection and quantification of Erwinia carotovora subsp. atroseptica on potato tubers. Lett. Appl. Microbiol. 30:330-35
56. Hyman LJ, Wallace A, Lopez MM, Cambra M, Gorris MT, Perombelon MCM. 1995. Characterization of monoclonal antibodies against Erwinia carotovora subsp. atroseptica Serogroup I: specificity and epitope analysis. J. Appl. Bacteriol. 78:437-44
57. Jaufeerally-Fakim Y, Autrey JC, Daniels MJ, Dookun A. 2000. Genetic polymorphism in Xanthomonas albilineans strains originating from 11 geographical locations, revealed by two DNA probes. Lett. Appl. Microbiol. 30:287-93
58. Jeng RS, Svircev AM, Myers AL, Beliaeva L, Hunter DM, Hubbes M. 2001. The use of 16S and 16S-23S rDNA to easily detect and differentiate common Gram-negative orchard epiphytes. J. Microbiol. Methods 44:69-77
59. Jimenez L, Smalls S. 2000. Molecular detection of Burkholderia cepacia in toiletry, cosmetic, and pharmaceutical raw materials and finished products. J. AOAC Int. 83:963-66
60. Jock S, Jacob T, Kim WS, Hildebrand M, Vosberg HP, Geider K. 2003. Instability of short-sequence DNA repeats of pear pathogenic Erwinia strains from Japan and Erwinia amylovora fruit tree and raspberry strains. Mol. Genet. Genomics 268:739-49
61. Jones JB, Somodi GC, Scott JW. 1997. Increased ELISA sensitivity using a modified extraction buffer for detection of Xanthomonas campestris pv. vesicatoria in leaf tissue. J. Appl. Microbiol. 83:397-401
62. Kaneshiro WS. 2003. Detection and characterization of virulent, hypovirulent, and nonvirulent Clavibacter michiganensis subsp. michiganensis. MSc. thesis. Univ. Hawaii Manoa, Honolulu. 88 pp.
63. Knirel YA, Ovod W, Zdorovenko GM, Gvozdyak RI, Krohn KJ. 1998. Structure of the O polysaccharide and immunochemical relationships between the lipopolysaccharides of Pseudomonas syringae pathovar tomato and pathovar maculicola. Eur. J. Biochem. 258:657-61
64. Koh YJ, Nou IS. 2002. DNA markers for identification of Pseudomonas syringae pv. actinidiae. Mol. Cells 13:309-14
65. Kong LR, Tzeng DD, Yang CH. 2001. Generation of PCR-based DNA fragments for specific detection of Streptomyces saraceticus N45. Proc. Natl. Sci. Counc. Repub. China B 25:119-27
66. Lamka GL, Hill JH, McGee DC, Braun EJ. 1991. Development of an immunosorbent assay for seedborne Erwinia stewartii in corn seeds. Phytopathology 81:839-46
67. Lee YA, Fan SC, Chiu LY, Hsia KC. 2001. Isolation of an insertion sequence from Ralstonia solanacearum race 1 and its potential use for strain characterization and detection. Appl. Environ. Microbiol. 67:3943-50
68. Leveau JHJ, Lindow SE. 2002. Bioreporters in microbial ecology. Curr. Opin. Microbiol. 5:259-65
69. Lin CP, Chen TA, Wells JM, Van der Zwet T. 1986. Monoclonal antibodies specific to Erwinia amylovora. Phytopathology 76:564 (Abstr.)
70. Lipp RL, Alvarez AM, Benedict AA, Berestecky J. 1992. Use of monoclonal antibodies and pathogenicity tests to characterize strains of Xanthomonas campestris pv. dieffenbachiae from aroids. Phytopathology 82:677-82
71. Llop P, Bonaterra A, Penalver J, Lopez MM. 2000. Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material. Appl. Environ. Microbiol. 66:2071-78
72. Lópes SA, Damann KE, Grelen LB. 2001. Xanthomonas albilineans diversity and identification based on rep-PCR fingerprints. Curr. Microbiol. 42:155-59
73. López MM, Bertolini E, Olmos A, Caruso P, Gorris MT, et al. 2003. Innovative tools for the detection of plant pathogenic viruses and bacteria. Int. Microbiol. 6:233-43
74. Louws FJ, Rademaker JLW, De Bruijn FJ. 1999. The three D's of PCR-based genomic analysis of phytobacteria: diversity, detection, and disease diagnosis. Annu. Rev. Phytopathol. 37:81-125
75. Macario AJL, Macario ECD. 1985. Monoclonal Antibodies Against Bacterial Introduction: Monoclonal Antibodies against Bacteria for Medicine, Dentistry, Veterinary Sciences, Biotechnology, and Industry - An Overview, pp. xvii-xxxiii. New York: Academic
76. Magee WE, Beck CF, Ristow SS. 1986. Monoclonal antibodies specific for Corynebacterium sepedonicum, the causative agent of potato ring rot. Hybridoma 5:231-35
77. Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, et al. 2000. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J. Clin. Microbiol. 38:3165-73
78. Manulis S, Chalupowicz L, Dror O, Kleitman F. 2002. Molecular diagnostic procedures for production of pathogen-free propagation material. Pest Manag. Sci. 58:1126-31
79. Manulis S, Valinsky L, Lichter A, Gabriel DW. 1994. Sensitive and specific detection of Xanthomonas campestris pv. pelargonii with DNA primers and probes identified by random amplified polymorphic DNA analysis. Appl. Environ. Microbiol. 60:4094-99
80. Martín RR, James D, Levésque AC. 2000. Impacts of molecular diagnostic technologies on plant disease management. Annu. Rev. Phytopathol. 38:207-39
81. Mc Garvey JA, Bell CJ, Denny TP, Schell MA. 1998. Analysis of extracellular polysaccharide I in culture and in plants using immunological methods: new insights and implications for phylogeny, diversity, and molecular diagnostics of Ralstonia solanacearum. In Bacterial Wilt Disease: Molecular and Ecological Aspects, ed. P Prior, C Allen, JG Elphinstone, pp. 157-63. Berlin: Springer-Verlag
82. McDowell A, Mahenthiralingam E, Moore JE, Dunbar KE, Webb AK, et al. 2001. PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. J. Clin. Microbiol 39:4247-55
83. Miller SC, LiPuma JJ, Parke JL. 2002. Culture-based and non-growth-dependent detection of the Burkholderia cepacia complex in soil environments. Appl. Environ. Microbiol. 68:3750-58
84. Mochizuki GT, Alvarez AM. 1996. A bioluminescent Xanthomonas campestris pv. campestris to monitor black rot infections in cabbage seedlings treated with fosetyl-Al. Plant Dis. 80:758-62
85. Momol MT, Momol EA, Lamboy WF, Norelli JL, Beer SV, Aldwinckle HS. 1997. Characterization of Erwinia amylavora strains using random amplified polymorphic DNA fragments (RAPDs). J. Appl. Microbiol. 82:389-98
86. Moore JE, Xu J, Millar BC, Crowe M, Elborn JS. 2002. Improved molecular detection of Burkholderia cepacia genomovar III and Burkholderia multivorans directly from sputum of patients with cystic fibrosis. J. Microbiol. Methods 49:183-91
87. Norman DJ, Alvarez AM. 1994. Rapid detection of Xanthomonas campestris pv. dieffenbachiae in anthurium plants with a miniplate enrichment/ELIS A system. Plant Dis. 78:954-58
88. Ovod V, Ashorn P, Yakovleva L, Krohn K. 1995. Classification of Pseudomonas syringae with monoclonal antibodies against the core and O-side chains of the lipopolysaccharide. Phytopathology 85:226-32
89. Ovod V, Rudolph K, Krohn K. 1997. Serological classification of Pseudomonas syringae pathovars based on monoclonal antibodies towards the lipopolysaccharide O-chains. In Pseudomonas syringae Pathovars and Related Pathogens, ed. K Rudolph, TJ Burr, JW Mansfield, D Stead, A Vivian, J von Kietzell, pp. 526-31. Dordrecht: Kluwer
90. 2 with monoclonal antibodies. J. Bacteriol. 178:6459-65
91. 1 strains. J. Bacteriol. 181:6937-47
92. Pastrik KH. 2000. Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by multiplex PCR with coamplification of host DNA. Eur. J. Plant Pathol. 106:155-65
93. Pastrik KH, Elphinstone JG, Pukall R. 2002. Sequence analysis and detection of Ralstonia solanacearum by multiplex PCR amplification of 16S-23S ribosomal intergenic spacer region with internal positive control. Eur. J. Plant Pathol. 108:831-42
94. Permar TA, Gottwald TR. 1989. Specific recognition of a Xanthomonas campestris Florida nursery strain by a monoclonal antibody probe in a microfiltration enzyme immunoassay. Phytopathology 79:780-83
95. Porteous LA, Widmer F, Seidler RJ. 2002. Multiple enzyme restriction fragment length polymorphism analysis for high resolution distinction of Pseudomonas (sensu stricto) 16S rRNA genes. J. Microbiol. Methods 51:337-48
96. Poussier S, Cheron JJ, Couteau A, Luisetti J. 2002. Evaluation of procedures for reliable PCR detection of Ralstonia solanacearum in common natural substrates. J. Microbiol. Methods 51:349-59
97. Poussier S, Trigalet-Demery D, Vandewalle P, Goffinet B, Luisetti J, Trigalet A. 2000. Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision. Microbiology 146 (Part 7):1679-92
98. Poussier S, Vandewalle P, Luisetti J. 1999. Genetic diversity of African and worldwide strains of Ralstonia solanacearum as determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region. Appl. Environ. Microbiol. 65:2184-94
99. Priou S, Gutarra L, Aley P. 1999. Highly sensitive detection of Ralstonia solanacearum in latently infected potato tubers by post-enrichment ELISA on nitrocellulose membrane. EPPO/OEPP Bull. 29:117-25
100. Robinson-Smith A, Jones P, Elphinstone JG, Forde SMD. 1995. Production of antibodies to Pseudomonas solanacearum, the causative agent of bacterial wilt. Food Agric. Immunol. 7:67-79
101. Sakthivel N, Mortensen CN, Mathur SB. 2001. Detection of Xanthomonas oryzae pv. oryzae in artificially inoculated and naturally infected rice seeds and plants by molecular techniques. Appl. Microbiol. Biotechnol. 56:435-41
102. Sanders GM, Verschoor JA, van Wyngaard S, Korsten L, Kotze JM. 1994. Production of monoclonal antibodies against Xanthomonas campestris pv. mangiferaeindicae and their use to investigate differences in virulence. J. Appl. Bacteriol. 77:509-18
103. Schaad NW, Berthier-Schaad Y, Sechler A, Knorr D. 1999. Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system. Plant Dis. 83: 1095-100
104. Schaad NW, Frederick RD, Shaw J, Schneider WL, Hickson R, et al. 2003. Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. Annu. Rev. Phytopathol. 41:305-24
105. Schaad NW, Jones JB, Chun W. 2002. Laboratory Guide for Identification of Plant Pathogenic Bacteria. St. Paul, MN: APS Press
106. Schaad NW, SC, Tamaki S, Hatziloukas E, Panopoulas N. 1995. A combined biological amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts. Phytopathology 85:243-48
107. Sheppard JW, ed. 1999. Proc. Third Int. Seed Health Symp. Ames, Iowa: Int. Seed Test. Assoc.
108. Shigaki T, Nelson SC, Alvarez AM. 2000. Symptomless spread of blight-inducing strains of Xanthomonas campestris pv. campestris on cabbage seedlings in misted seedbeds. Eur. J. Plant Pathol. 106:339-46
109. Singh U, Trevors CM, De Boer SH, Janse JD. 1999. Fimbrial-specific monoclonal antibody-based ELISA for European potato strains of Erwinia chrysanthemi and comparison to PCR. Plant Dis. 84:443-8
110. Stead DE, Elphinstone JG, Simpkins S, Weller SA. 2002. Detection of plant pathogenic bacteria by real-time PCR. Phytopathology 92:S110
111. Stowell LJ, Gelernter WD. 2001. Diagnosis of turfgrass diseases. Annu Rev. Phytopathol. 39:135-55
112. Thaler ER, Kennedy DW, Hanson CW. 2001. Medical applications of electronic nose technology: review of current status. Am. J. Rhinol. 15:291-5
113. Toth IK, Avrova AO, Hyman LJ. 2001. Rapid identification and differentiation of the soft rot erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses. Appl. Environ. Microbiol. 67:4070-76
114. Toth IK, Bertheau Y, Hyman LJ, Laplaze L, Lopez MM, et al. 1999. Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotavora subsp. atroseptica. J. Appl. Microbiol. 87:770-81
115. Trebaol G, Gardan L, Manceau C, Tanguy JL, Tirilly Y, Boury S. 2000. Genomic and phenotypic characterization of Xanthomonas cynarae sp. nov., a new species that causes bacterial bract spot of artichoke (Cynara scolymus L.). Int. J. Syst. Evol. Microbiol. 50(Part 4):1471-78
116. Tsai CC, Lin CP, Chen CT. 1990. Characterization and monoclonal antibody production of Xanthomonas albilineans (Ashby) Dowson, the causal agent of sugarcane leaf scald disease. Plant Protect. Bull. 32:125-35
117. Tungpradabkul S, Wajanarogana S, Tunpiboonsak S, Panyim S. 1999. PCR-RFLP analysis of the flagellin sequences for identification of Burkholderia pseudomallei and Burkholderia cepacia from clinical isolates. Mol. Cell Probes 13:99-105
118. Van Beckhoven JRCM, Stead DE, Van der Wolf JM. 2002. Detection of Clavibacter michiganensis subsp. sepedonicus by AmpliDet RNA, a new technology based on real time monitoring of NASBA amplicons with a molecular beacon. J. Appl. Microbiol. 93:840-49
119. Van der Wolf JM, Hyman LJ, Jones DA, Grevesse C, van Beckhoven JR, et al. 1996. Immunomagnetic separation of Erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on a crystal violet pectate medium or by PCR. J. Appl. Bacteriol. 80:487-95
120. van der Wolf JM, Hyman LJ, Jones DAC, Grevesse C, van Beckhoven JRCM, et al. 1996. Immunomagnetic separation of Erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on a crystal violet pectate medium or by PCR. J. Appl. Bacteriol. 80:487-95
121. Van der Wolf JM, Schoen CD. 2004. Bacterial Pathogens: Detection and Identification Methods, pp. 1-5. New York: Marcel Dekker
122. Van der Wolf JM, Sledz V, Van Elsas JD, Van Overbeek L, Van Bergervoet JHW. 2004. Flow cytometry to detect Ralstonia solanacearum and to assess viability. In Bacterial Wilt: The Disease and the Ralstonia solanacearum Species Complex, ed. C Allen, P Prior, p. 6. St. Paul, MN: APS Press
123. Van der Wolf JM, Van Beckhoven JRCM, De Haan EG, Van den Bovenkamp GW. 2004. Specific detection of Ralstonia solanacearum 16S rRNA se quences by AmpliDet RNA. Eur. J. Plant Pathol. 110:25-33
124. Van Doom J, Hollinger TC, Oudega B. 2001. Analysis of the type IV fimbrial-subunit gene fimA of Xanthomonas hyacinthi: application in PCR-mediated detection of yellow disease in Hyacinths. Appl. Environ. Microbiol. 67:598-607
125. Van Overbeek LS, Cassidy M, Kozdroj J, Trevors JT, Van Elsas JD. 2002. A polyphasic approach for studying the interaction between Ralstonia solanacearum and potential control agents in the tomato phytosphere. J. Microbiol. Methods 48:69-86
126. Van Sluys MA, Monteiro-Vitorello CB, Camargo LEA, Menck CFM, da Silva ACR, et al. 2001. Comparative genomic analysis of plant-associated bacteria. Annu. Rev. Phytopathol. 40:169-89
127. Van Vuurde JWL, Van der Wolf JM. 1995. Immunofluorescence colony-staining. In Molecular Microbiology Ecology Manual, ed. ADI Akkermans, JD Van Elsas, FJ De Bruijn, pp. 1-19. Dordrecht: Kluwer
128. Vandamme P, Pot B, Gillis M, De Vos P, Kersters K, Swings J. 1996. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60:407-38
129. Vauterin L, Hoste B, Yang P, Alvarez AM, Kersters K, Swings J. 1993. Xanthomonas, pp. 157-92. London/New York: Chapman & Hall
130. Veena MS, van Vuurde JW. 2002. Indirect immunofluorescence colony staining method for detecting bacterial pathogens of tomato. J. Microbiol. Methods 49:11-17
131. Vernon-Shirley M, Burns R. 1992. The development and use of monoclonal antibodies for detection of Erwinia. J. Appl. Bacteriol. 72:97-102
132. Vine BG, Kaneshiro WS, Alvarez AM. 2003. T/DGGE differentiation of virulent Clavibacter michiganensis subsp. michiganensis (Cmm) from nonvirulent Clavibacter-like saprophytes. Phytopathology 94: Abstr.
133. Volk WA. 1966. Cell wall lipopolysaccharides from Xanthomonas species. J. Bacteriol. 91:39-42
134. Walcott RR, Gitaitis RD. 2000. Detection of Acidovorax avenae subsp. citrulli in watermelon seed using immunomagnetic separation and the polymerase chain reaction. Plant Dis. 84:470-74
135. Waleron M, Waleron K, Podhajska AJ, Lojkowska E. 2002. Genotyping of bacteria belonging to the former Erwinia genus by PCR-RFLP analysis of a recA gene fragment. Microbiology 148:583-95
136. Wallace HR. 1978. The diagnosis of plant diseases of complex etiology. Annu. Rev. Phytopathol. 16:379-402
137. Weller SA, Elphinstone JG, Smith NC, Boonham N, Stead DE. 2000. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66:2853-58
138. Weller SA, Elphinstone JG, Smith N, Stead DE, Boonham N. 1999. Detection of Ralstonia solanacearum strains using an automated and quantitative fluorogenic 5′ nuclease TaqMan assay. Appl. Environ. Microbiol. 66:2853-58
139. Weng SF, Chen CY, Lee YS, Lin JW, Tseng YH. 1999. Identification of a novel beta-lactamase produced by Xanthomonas campestris, a phytopathogenic bacterium. Antimicrob. Agents Chemother. 43:1792-97
140. Whitby PW, Carter KB, Burns JL, Royall JA, LiPuma JJ, Stull TL. 2000. Identification and detection of Stenotrophomonas maltophilia by rRNA-directed PCR. J. Clin. Microbiol. 38:4305-9
141. Whitby PW, Carter KB, Hatter KL, LiPuma JJ, Stull TL. 2000. Identification of members of the Burkholderia cepacia complex by species-specific PCR. J. Clin. Microbiol. 38:2962-65
142. Whitby PW, Dick HL, Campbell PW 3rd, Tullis DE, Matlow A, Stull TL. 1998. Comparison of culture and PCR for detection of Burkholderia cepacia in sputum samples of patients with cystic fibrosis. J. Clin. Microbiol. 36:1642-45
143. Wilson AD, Lester DG. 1997. Use of an electronic-nose device for profiling headspace volatile metabolites to rapidly identify phytopathogenic microbes. Phytopathology 87:S116
144. Wong WC. 1990. Production of monoclonal antibodies to Pseudomonas syringae pv. phaseolicola and Xanthomonas campestris pv. phaseoli. Lett. Appl. Microbiol. 10:241-44
145. Zdorovenko EL, Zatonskii GV, Kocharova NA, Shashkov AS, et al. 2003. Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri. Eur. J. Biochem. 270:20-27