Production and characterization of monoclonal antibodies specific for erwinia amylovora and their use in different serological techniques

Acta Horticulturae

Volumn 411, Issue 1, 1996, Pages 47-51

  Gorris, M T ^a   Camarasa, E ^a   Lopez M M ^a   Cambra, M ^a   Paulin, J P ^b   Chartier, R ^b  

^a INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^b INRA   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ERWINIA AMYLOVORA; MALUS X DOMESTICA; PAPAYA MOSAIC VIRUS;

EID: 4744347476     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.1996.411.11     Document Type: Conference Paper

References (13)

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2. Alarcon, B., Lopez, M.M., Cambra M. and Ortiz, J. 1987. Comparative study of Agrobacterium biotypes 1, 2 and 3 by electrophoresis and serological methods. Journal of Applied Bacteriology 62, 295-308.
3. Bennett, R.A. and Billing, E. 1980. Origin of the polysaccharide component of ooze from plants infected with Erwinia amylovora. Journal of General Microbiology 116: 341-349.
4. Cambra, M., Cambra, M.A., Gorris, M.T. and Lopez, M.M. 1992. Localization of phytopathogenic bacterial antigens in plant tissue sections by immunoprinting ELISA using biotinilated monoclonal antibodies. In: Plant Pathogenic Bacteria, Les Colloques INRA; Paris. 66: 331-335.
5. Cambra, M., Llacer G., Perez de San Roman, C, Moreno, P. and Durba, V. 1983. Diagnostico rapido de virus en frutales de hueso mediante la tecnica ELISA-DAS. Bol. Serv. Plagas 9: 45-59.
6. De Boer, S.H. 1990. Immunofluorescence. Bacteria, In: Serological methods for detection and identification of viral and bacterial plant pathogens. APS Press, St. Paul, Minn.: 295-298.
7. Gorris, M.T., Alarcon, B., Lopez, M.M. and Cambra, M. 1994. Characterization of monoclonal antibodies specific for Erwinia carotovora subsp. atroseptica and comparison of serological methods for its sensitive detection on potato tubers. Applied and Environmental Microbiology 60: 2076-2085.
8. Gugerli, P. and Gouk S.C. 1992. Identification of Erwinia amylovora with monoclonal antibodies. In: Plant Pathogenic Bacteria, Les Colloques INRA, Paris. 66: 325-330.
9. Hutschemackers, J., Verhoyen, M. and Bazin H. 1987. Production of rat monoclonal antibodies specific to Erwinia amylovora. Acta Horticulturae 217: 71-76.
10. Kbhler, G. and Milstein, C. 1975. Continuous cultures of fused cell secreting antibodies of predefined specificity. Nature, UK 256: 495-497.
11. Lin, N.S., Su, Y.H. and Hsu, H.T. 1990. Immunological detection of plant viruses and mycoplasmalike organisms by direct tissue blotting on nitrocellulose membranes. Phytopathology 80: 824-828.
12. McLaughlin, R.J., Chen T.A. and Wells, J.M. 1989. Monoclonal antibodies against Erwinia amylovora: characterization and evaluation of a mixture for detection by enzyme-linked immunosorbent assay. Phytopathology 79: 610-613.
13. Siverio, F., Cambra, M., Gorris, M.T., Corzo, J. and Lopez, M.M. 1993. Lipopolysaccharides as determinants of serological variability in Pseudomonas corrugata. Applied and Environmental Microbiology, 59: 1805-1812.