Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers

Applied and Environmental Microbiology

Volumn 68, Issue 7, 2002, Pages 3634-3638

  Caruso, Paola ^a   Gorris, María Teresa ^a   Cambra, Mariano ^a   Palomo, José Luis ^a   Collar, Jesús ^a   López, María M ^a  

^a INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; ENZYMES; MONOCLONAL ANTIBODIES;

IMMUNOSORBENTS;

MICROORGANISMS;

MONOCLONAL ANTIBODY;

BACTERIUM; TUBER;

ACCURACY; ANTIBODY PRODUCTION; ARTICLE; BACTERIUM IDENTIFICATION; DOUBLE ANTIBODY SANDWICH ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; POTATO; RALSTONIA SOLANACEARUM;

ANTIBODIES, MONOCLONAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; PROTEOBACTERIA; SOLANUM TUBEROSUM;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; RALSTONIA; RALSTONIA SOLANACEARUM; SOLANUM TUBEROSUM; UNIDENTIFIED BACTERIUM;

EID: 0036301252     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.7.3634-3638.2002     Document Type: Article

References (27)

1. Differentation of Erwinia carotovora subsp. carotovora and Erwinia carotovora subsp. atroseptica isolated from potato by Western blot and subsequent indirect ELISA
2. Serological characterization of potato isolates of Erwinia carotovora subsp. atroseptica and subsp. carotovora using polyclonal and monoclonal antibodies
3. Serological and molecular approaches to identification of Pseudomonas solanacearum strains from Heliconia
4. Ralstonia solanacearum race 3
5. Evaluation of methods for detection of potato seed contamination by Ralstonia solanacearum
6. Immunosorbent assays in plant pathology
7. Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tubers extracts
8. Standardization of methods for detection of Ralstonia solanacearum in potato
9. Modifications of a semi-selective medium for the isolation of Pseudomonas solanacearum
10. Characterization of monoclonal antibodies specific for Erwinia carotovora subsp. atroseptica and comparison of serological methods for its selective detection on potato tubers
11. Survival of Pseudomonas solanacearum race 3 in plant debris and latently infected potato tubers
12. Development of specific recombinant monoclonal antibodies against the lipopolysaccharide of Ralstonia solanacearum race 3
13. Round table on bacterial wilt (brown rot) of potato
14. Bacterial wilt in the People's Republic of China
15. A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity
16. The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium
17. The role of motility and aerotaxis in the selective increase of avirulent bacteria in still broth culture of Pseudomonas solanacearun
18. Continuous cultures of fused cell secreting antibodies of predefined specificity
19. The aluminum-cap methods for testing sugarcane varieties against leaf scald disease
20. Enrichment ELISA (E-ELISA)
21. Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria
22. Strategies for improving serological and molecular detection of plant pathogenic bacteria
23. Highly sensitive detection of Ralstonia solanacearum in latently infected potato tubers by postenrichment enzyme-linked immunosorbent assay on nitrocellulose membrane
24. Production of antibodies to Ralstonia solanacearum, the causative agent of bacterial wilt
25. Differentiation of Pseudomonas solancearum, Pseudomonas syzgii, Pseudomonas pickettii, and blood disease bacterium by partial 16S sequencing: Construction of oligonucleotide primers for sensitive detection by polymerase chain reaction
26. Lipopolysaccharides as determinants of serological variability in Pseudomonas corrugata
27. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov. Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov.